KU80_YARLI
ID KU80_YARLI Reviewed; 726 AA.
AC Q6C7B9;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=ATP-dependent DNA helicase II subunit 2;
DE EC=3.6.4.12;
DE AltName: Full=ATP-dependent DNA helicase II subunit Ku80;
GN Name=KU80; OrderedLocusNames=YALI0E02068g;
OS Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Dipodascaceae; Yarrowia.
OX NCBI_TaxID=284591;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CLIB 122 / E 150;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: Single-stranded DNA-dependent ATP-dependent helicase.
CC Involved in non-homologous end joining (NHEJ) DNA double strand break
CC repair. DNA-binding is sequence-independent but has a high affinity to
CC nicks in double-stranded DNA and to the ends of duplex DNA. Binds to
CC naturally occurring chromosomal ends, and therefore provides
CC chromosomal end protection. Required also for telomere recombination to
CC repair telomeric ends in the absence of telomerase. KU70, of the
CC KU70/KU80 heterodimer, binds to the stem loop of TLC1, the RNA
CC component of telomerase. Involved in telomere maintenance. Interacts
CC with telomeric repeats and subtelomeric sequences thereby controlling
CC telomere length and protecting against subtelomeric rearrangement.
CC Maintains telomeric chromatin, which is involved in silencing the
CC expression of genes located at the telomere. Required for mating-type
CC switching (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SUBUNIT: Heterodimer of Ku70 and Ku80. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ku80 family. {ECO:0000305}.
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DR EMBL; CR382131; CAG79022.1; -; Genomic_DNA.
DR RefSeq; XP_503443.1; XM_503443.1.
DR AlphaFoldDB; Q6C7B9; -.
DR SMR; Q6C7B9; -.
DR STRING; 4952.CAG79022; -.
DR EnsemblFungi; CAG79022; CAG79022; YALI0_E02068g.
DR GeneID; 2912079; -.
DR KEGG; yli:YALI0E02068g; -.
DR VEuPathDB; FungiDB:YALI0_E02068g; -.
DR HOGENOM; CLU_010975_1_1_1; -.
DR InParanoid; Q6C7B9; -.
DR OMA; WAMQYVW; -.
DR Proteomes; UP000001300; Chromosome E.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0043564; C:Ku70:Ku80 complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0042162; F:telomeric DNA binding; IBA:GO_Central.
DR GO; GO:0071480; P:cellular response to gamma radiation; IBA:GO_Central.
DR GO; GO:0071481; P:cellular response to X-ray; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IBA:GO_Central.
DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central.
DR CDD; cd00873; KU80; 1.
DR Gene3D; 2.40.290.10; -; 1.
DR Gene3D; 3.40.50.410; -; 1.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR024193; Ku80.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR InterPro; IPR036465; vWFA_dom_sf.
DR Pfam; PF02735; Ku; 1.
DR PIRSF; PIRSF016570; Ku80; 1.
DR SMART; SM00559; Ku78; 1.
DR SUPFAM; SSF100939; SSF100939; 1.
DR SUPFAM; SSF53300; SSF53300; 1.
PE 3: Inferred from homology;
KW ATP-binding; Chromosome; DNA damage; DNA recombination; DNA repair;
KW DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; Telomere.
FT CHAIN 1..726
FT /note="ATP-dependent DNA helicase II subunit 2"
FT /id="PRO_0000278356"
FT DOMAIN 248..475
FT /note="Ku"
FT REGION 591..620
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 726 AA; 81612 MW; EDF19A198B33B969 CRC64;
MASKEATVYV VDLHESMGRE MARGHDSKEV TDLNWGLQYI YSEISNKILS GRKTDVVGLV
GVHTDTTQNM FEEESGFEHI DIITPIQQFN LDTLLEAKKQ LVPNSDNKGD LISGIVVAVQ
MIKLYTKALK YIRNIVVLTN GQGNMNLGDS GGIIKQLNEN RIILKVMGVD FDDEEVDYFE
EDKPEHKREN ELKLKEFVDR CEDSVFATYK EARDSLDIPK VKAVNPVRAF QGNLVLSDPE
QQPPQRVMSI GVEVFPCTRR ATAMTASSYA MSKIEPATIS TPSQNNLQAV KWDRQYYVND
ESGIGGKKEL DRDTLENGYR YGSEIVYITK EEEEAIMFPT SASLQVIGFV NKKSVPPYML
MGHTDYIIGQ RGNNRDAVAI SAFARALFET DNFGLARYVN KDGKDPQIVV LMPYIRAELE
GLVFCQLPFA EDERKFILPS LTSLETRSGN KTVTTHSRLL PTKEMLDAMD DYVDAMDLSK
LKGEDDEPWL TMEECFNPSI HHIRNVVKEC AVSQDYGKIP EPLPILTRFS QPAEELTEEA
KPQLELLKHL FDIKEQFREA KKRKTEVTVG QTGLDLDALL EGELEVKLES SQTLSSNVKS
EPGRQTASQS SQNSQQLSLD PENICPDFIR TLNKIDTKAT SDEEFRSGAT NLYKQTITLL
EEKLESSKGN DAYNEIIGVL QTMREQADEM EIPDVFTAAK GQFVSKLERG DLGGQKNVLV
AQINSI
