KUA1_ARATH
ID KUA1_ARATH Reviewed; 365 AA.
AC Q9LVS0;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 162.
DE RecName: Full=Transcription factor KUA1 {ECO:0000303|PubMed:24806884};
DE AltName: Full=Myb-related protein H {ECO:0000303|PubMed:23888064};
DE Short=AtMYBH {ECO:0000303|PubMed:23888064};
DE Short=AtMYBS3 {ECO:0000303|PubMed:25920996};
DE Short=MYBS3-homolog protein {ECO:0000303|PubMed:25920996};
DE AltName: Full=Protein KUODA1 {ECO:0000303|PubMed:24806884};
GN Name=KUA1 {ECO:0000303|PubMed:24806884};
GN Synonyms=MYBH {ECO:0000303|PubMed:23888064};
GN OrderedLocusNames=At5g47390 {ECO:0000312|Araport:AT5G47390};
GN ORFNames=MQL5.25 {ECO:0000312|EMBL:BAA97173.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: A genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP INDUCTION BY CDCL2, AND GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [8]
RP TRANSCRIPTION REPRESSION MOTIF.
RX PubMed=19324928; DOI=10.1093/pcp/pcp048;
RA Ikeda M., Ohme-Takagi M.;
RT "A novel group of transcriptional repressors in Arabidopsis.";
RL Plant Cell Physiol. 50:970-975(2009).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY DARK, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=23888064; DOI=10.1093/jxb/ert223;
RA Kwon Y., Kim J.H., Nguyen H.N., Jikumaru Y., Kamiya Y., Hong S.-W., Lee H.;
RT "A novel Arabidopsis MYB-like transcription factor, MYBH, regulates
RT hypocotyl elongation by enhancing auxin accumulation.";
RL J. Exp. Bot. 64:3911-3922(2013).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, INDUCTION DURING LEAF
RP EXPANSION, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=24806884; DOI=10.1038/ncomms4767;
RA Lu D., Wang T., Persson S., Mueller-Roeber B., Schippers J.H.M.;
RT "Transcriptional control of ROS homeostasis by KUODA1 regulates cell
RT expansion during leaf development.";
RL Nat. Commun. 5:3767-3767(2014).
RN [11]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY DARK AND DURING SENESCENCE,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=25920996; DOI=10.1007/s11103-015-0321-2;
RA Huang C.-K., Lo P.-C., Huang L.-F., Wu S.-J., Yeh C.-H., Lu C.-A.;
RT "A single-repeat MYB transcription repressor, MYBH, participates in
RT regulation of leaf senescence in Arabidopsis.";
RL Plant Mol. Biol. 88:269-286(2015).
CC -!- FUNCTION: Transcriptional repressor (PubMed:23888064, PubMed:24806884).
CC Direct regulator of the transcription of peroxidase (Prxs) and reactive
CC oxygen species (ROS)-related genes via the recognition of 5'-ATCACA-3'
CC motif (PubMed:24806884). Binds to 5'-TATCCA-3' motif (TA box) and
CC represses the activity of corresponding promoters (e.g. sugar response
CC genes) (PubMed:25920996). Regulates hypocotyl elongation in response to
CC darkness by enhancing auxin accumulation in a phytochrome-interacting
CC factor (PIF) proteins-dependent manner. Promotes lateral roots
CC formation (PubMed:23888064). Promotes cell expansion during leaves
CC development via the modulation of cell wall-located Prxs
CC (PubMed:24806884). Plays a critical role in developmentally regulated
CC and dark-induced onset of leaf senescence by repressing the
CC transcription of several genes involved in chloroplast function and
CC responses to light and auxin. Promotes responses to auxin, abscisic
CC acid (ABA), and ethylene (PubMed:25920996).
CC {ECO:0000269|PubMed:23888064, ECO:0000269|PubMed:24806884,
CC ECO:0000269|PubMed:25920996}.
CC -!- INTERACTION:
CC Q9LVS0; O64647: TCP9; NbExp=3; IntAct=EBI-15200088, EBI-9838721;
CC Q9LVS0; Q9XGN1: TTG1; NbExp=3; IntAct=EBI-15200088, EBI-395803;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:23888064, ECO:0000269|PubMed:24806884}.
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously, except in hypocotyls, root
CC tips and lateral root primordia. {ECO:0000269|PubMed:25920996}.
CC -!- DEVELOPMENTAL STAGE: Accumulates during leaf expansion. First observed
CC at the tip of the leaves 12 days after sowing (DAS). At 14 DAS,
CC expressed throughout the leaf blade to fade out thereafter in a
CC basipetal manner. In mature leaves, detected in vascular tissue,
CC especially in companion cells (PubMed:24806884). Accumulates to higher
CC levels in old rosette leaves than in young rosette and cauline leaves
CC (PubMed:25920996). {ECO:0000269|PubMed:24806884,
CC ECO:0000269|PubMed:25920996}.
CC -!- INDUCTION: Slightly induced by CdCl(2) (PubMed:16463103). Accumulates
CC in the dark (PubMed:23888064, PubMed:25920996). Diurnal expression
CC pattern with maximal levels in the morning (at protein level).
CC Specifically induced during leaf expansion (PubMed:24806884). Expressed
CC in old and dark-treated leaves (PubMed:25920996).
CC {ECO:0000269|PubMed:16463103, ECO:0000269|PubMed:23888064,
CC ECO:0000269|PubMed:24806884, ECO:0000269|PubMed:25920996}.
CC -!- DOMAIN: Contains a R/KLFGV repression motif, which may be involved in
CC the activity of transcriptional repression.
CC {ECO:0000303|PubMed:19324928}.
CC -!- DISRUPTION PHENOTYPE: Defects in root growth. No visible hypocotyl
CC phenotypes. Increased sensitivity to the gibberellic acid (GA)
CC biosynthesis inhibitor paclobutrazol (PAC) and auxin (IAA) that inhibit
CC hypocotyl elongation (PubMed:23888064). Reduced leaf size due to
CC impaired cell expansion associated with an enhanced expression of
CC peroxidase (Prxs) genes. This phenotype is reversed by SHAM treatment,
CC a peroxidase inhibitor. Increased accumulation of H(2)O(2)
CC (PubMed:24806884). Delayed senescence. Enhanced auxin-responsive gene
CC expression (PubMed:25920996). {ECO:0000269|PubMed:23888064,
CC ECO:0000269|PubMed:24806884, ECO:0000269|PubMed:25920996}.
CC -!- MISCELLANEOUS: 'Kuoda' means 'enlarge' or 'expand' in Chinese.
CC {ECO:0000305|PubMed:24806884}.
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DR EMBL; AY519516; AAS09986.1; -; mRNA.
DR EMBL; AB018117; BAA97173.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95505.1; -; Genomic_DNA.
DR EMBL; AY072077; AAL59900.1; -; mRNA.
DR EMBL; AY096661; AAM20295.1; -; mRNA.
DR EMBL; AK226931; BAE99002.1; -; mRNA.
DR EMBL; AY084295; AAM60886.1; -; mRNA.
DR RefSeq; NP_199550.1; NM_124110.4.
DR AlphaFoldDB; Q9LVS0; -.
DR IntAct; Q9LVS0; 5.
DR STRING; 3702.AT5G47390.1; -.
DR PaxDb; Q9LVS0; -.
DR PRIDE; Q9LVS0; -.
DR ProteomicsDB; 237037; -.
DR EnsemblPlants; AT5G47390.1; AT5G47390.1; AT5G47390.
DR GeneID; 834786; -.
DR Gramene; AT5G47390.1; AT5G47390.1; AT5G47390.
DR KEGG; ath:AT5G47390; -.
DR Araport; AT5G47390; -.
DR TAIR; locus:2171519; AT5G47390.
DR eggNOG; ENOG502QUPG; Eukaryota.
DR HOGENOM; CLU_038424_0_0_1; -.
DR InParanoid; Q9LVS0; -.
DR OMA; WPGYSTE; -.
DR OrthoDB; 1071185at2759; -.
DR PhylomeDB; Q9LVS0; -.
DR PRO; PR:Q9LVS0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVS0; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0010252; P:auxin homeostasis; IMP:UniProtKB.
DR GO; GO:0048527; P:lateral root development; IMP:UniProtKB.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0010150; P:leaf senescence; IMP:UniProtKB.
DR GO; GO:2000469; P:negative regulation of peroxidase activity; IMP:TAIR.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0030307; P:positive regulation of cell growth; IMP:TAIR.
DR GO; GO:0090697; P:post-embryonic plant organ morphogenesis; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IMP:UniProtKB.
DR GO; GO:0009646; P:response to absence of light; IEP:UniProtKB.
DR GO; GO:0009733; P:response to auxin; IMP:UniProtKB.
DR GO; GO:0009723; P:response to ethylene; IMP:UniProtKB.
DR GO; GO:0009739; P:response to gibberellin; IBA:GO_Central.
DR CDD; cd00167; SANT; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR006447; Myb_dom_plants.
DR InterPro; IPR001005; SANT/Myb.
DR InterPro; IPR017884; SANT_dom.
DR InterPro; IPR001878; Znf_CCHC.
DR Pfam; PF00249; Myb_DNA-binding; 1.
DR SMART; SM00717; SANT; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR TIGRFAMs; TIGR01557; myb_SHAQKYF; 1.
DR PROSITE; PS51294; HTH_MYB; 1.
DR PROSITE; PS50158; ZF_CCHC; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..365
FT /note="Transcription factor KUA1"
FT /id="PRO_0000439178"
FT DOMAIN 90..146
FT /note="HTH myb-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT ZN_FING 3..20
FT /note="CCHC-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT DNA_BIND 118..142
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 41..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 214..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 321..365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 24..28
FT /note="R/KLFGV (transcriptional repression)"
FT /evidence="ECO:0000303|PubMed:19324928"
FT COMPBIAS 41..64
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..359
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 365 AA; 39671 MW; 6BD7771C0A8EDE78 CRC64;
MTRRCSHCNH NGHNSRTCPN RGVKLFGVRL TEGSIRKSAS MGNLSHYTGS GSGGHGTGSN
TPGSPGDVPD HVAGDGYASE DFVAGSSSSR ERKKGTPWTE EEHRMFLLGL QKLGKGDWRG
ISRNYVTTRT PTQVASHAQK YFIRQSNVSR RKRRSSLFDM VPDEVGDIPM DLQEPEEDNI
PVETEMQGAD SIHQTLAPSS LHAPSILEIE ECESMDSTNS TTGEPTATAA AASSSSRLEE
TTQLQSQLQP QPQLPGSFPI LYPTYFSPYY PFPFPIWPAG YVPEPPKKEE THEILRPTAV
HSKAPINVDE LLGMSKLSLA ESNKHGESDQ SLSLKLGGGS SSRQSAFHPN PSSDSSDIKS
VIHAL
