ID   KUN3_TABYA              Reviewed;          76 AA.
AC   C8YJ95;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-NOV-2009, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Tabkunin 2 {ECO:0000303|PubMed:19531497};
DE   AltName: Full=Serine protease inhibitor 3 {ECO:0000312|EMBL:ACS72290.1};
DE   Flags: Precursor;
OS   Tabanus yao (Horsefly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Tabanomorpha; Tabanoidea;
OC   Tabanidae; Tabanus.
OX   NCBI_TaxID=485572;
RN   [1] {ECO:0000312|EMBL:ACS72290.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-51, SUBCELLULAR
RP   LOCATION, MASS SPECTROMETRY, AND FUNCTION.
RC   TISSUE=Salivary gland;
RX   PubMed=19531497; DOI=10.1074/mcp.m900186-mcp200;
RA   Ma D., Wang Y., Yang H., Wu J., An S., Gao L., Xu X., Lai R.;
RT   "Anti-thrombosis repertoire of blood-feeding horsefly salivary glands.";
RL   Mol. Cell. Proteomics 8:2071-2079(2009).
CC   -!- FUNCTION: Potent anticoagulant protein that inhibits the hydrolytic
CC       activities of all serine proteases tested (trypsin, thrombin, elastase,
CC       and chymotrypsin), with the highest efficacy on thrombin.
CC       {ECO:0000269|PubMed:19531497}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19531497}.
CC   -!- TISSUE SPECIFICITY: Expressed in salivary glands.
CC       {ECO:0000305|PubMed:19531497}.
CC   -!- MASS SPECTROMETRY: Mass=6015.1; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:19531497};
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DR   EMBL; FJ469602; ACS72290.1; -; mRNA.
DR   MEROPS; I02.026; -.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Blood coagulation cascade inhibiting toxin; Direct protein sequencing;
KW   Disulfide bond; Hemostasis impairing toxin; Protease inhibitor; Secreted;
KW   Serine protease inhibitor; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000305|PubMed:19531497"
FT   CHAIN           21..76
FT                   /note="Tabkunin 2"
FT                   /evidence="ECO:0000305|PubMed:19531497"
FT                   /id="PRO_5002994254"
FT   DOMAIN          25..75
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        25..75
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        34..58
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        50..71
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   76 AA;  8202 MW;  B76DC45A681DE653 CRC64;
     MKVLSLIFVI FSVLVLFASA KDPVCDQPKA VGRCFAAFPK FYFNSSSGQC EAFIYGGCGG
     NENNFSTLEE CNAKCA