ID   KUN4_TABYA              Reviewed;          76 AA.
AC   C8YJ96;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-NOV-2009, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Tabkunin 4 {ECO:0000303|PubMed:19531497};
DE   AltName: Full=Serine protease inhibitor 4 {ECO:0000312|EMBL:ACS72291.1};
DE   Flags: Precursor;
OS   Tabanus yao (Horsefly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Tabanomorpha; Tabanoidea;
OC   Tabanidae; Tabanus.
OX   NCBI_TaxID=485572;
RN   [1] {ECO:0000312|EMBL:ACS72291.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Salivary gland;
RX   PubMed=19531497; DOI=10.1074/mcp.m900186-mcp200;
RA   Ma D., Wang Y., Yang H., Wu J., An S., Gao L., Xu X., Lai R.;
RT   "Anti-thrombosis repertoire of blood-feeding horsefly salivary glands.";
RL   Mol. Cell. Proteomics 8:2071-2079(2009).
CC   -!- FUNCTION: Potent anticoagulant protein that inhibits the hydrolytic
CC       activities of all serine proteases tested (trypsin, thrombin, elastase,
CC       and chymotrypsin), with the highest efficacy on thrombin.
CC       {ECO:0000250|UniProtKB:C1IBY4}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:19531497}.
CC   -!- TISSUE SPECIFICITY: Expressed in salivary glands.
CC       {ECO:0000305|PubMed:19531497}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FJ469603; ACS72291.1; -; mRNA.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   3: Inferred from homology;
KW   Blood coagulation cascade inhibiting toxin; Disulfide bond;
KW   Hemostasis impairing toxin; Protease inhibitor; Secreted;
KW   Serine protease inhibitor; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..76
FT                   /note="Tabkunin 4"
FT                   /id="PRO_5002994495"
FT   DOMAIN          25..75
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        25..75
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        34..58
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
SQ   SEQUENCE   76 AA;  8231 MW;  186DDA332A7D8B88 CRC64;
     MKVLSLIFVI FSVLVLFASA KDPVCDQPKA VGRCFAAFPK FYFNSSSAQG EAFFYGGCGG
     NENNFNTLEE CNAKCA