KU_ACTMD
ID KU_ACTMD Reviewed; 286 AA.
AC C6W9U8;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN Name=ku {ECO:0000255|HAMAP-Rule:MF_01875}; OrderedLocusNames=Amir_3417;
OS Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / JCM 3225 / NBRC 14064
OS / NCIMB 13271 / NRRL B-12336 / IMRU 3971 / 101).
OC Bacteria; Actinobacteria; Pseudonocardiales; Pseudonocardiaceae;
OC Actinosynnema.
OX NCBI_TaxID=446462;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29888 / DSM 43827 / JCM 3225 / NBRC 14064 / NCIMB 13271 / NRRL
RC B-12336 / IMRU 3971 / 101;
RX PubMed=21304636; DOI=10.4056/sigs.21137;
RA Land M., Lapidus A., Mayilraj S., Chen F., Copeland A., Del Rio T.G.,
RA Nolan M., Lucas S., Tice H., Cheng J.F., Chertkov O., Bruce D., Goodwin L.,
RA Pitluck S., Rohde M., Goker M., Pati A., Ivanova N., Mavromatis K.,
RA Chen A., Palaniappan K., Hauser L., Chang Y.J., Jeffries C.C., Brettin T.,
RA Detter J.C., Han C., Chain P., Tindall B.J., Bristow J., Eisen J.A.,
RA Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Actinosynnema mirum type strain (101).";
RL Stand. Genomic Sci. 1:46-53(2009).
CC -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC {ECO:0000255|HAMAP-Rule:MF_01875}.
CC -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC Rule:MF_01875}.
CC -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC Rule:MF_01875}.
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DR EMBL; CP001630; ACU37315.1; -; Genomic_DNA.
DR AlphaFoldDB; C6W9U8; -.
DR STRING; 446462.Amir_3417; -.
DR EnsemblBacteria; ACU37315; ACU37315; Amir_3417.
DR KEGG; ami:Amir_3417; -.
DR eggNOG; COG1273; Bacteria.
DR HOGENOM; CLU_048975_1_1_11; -.
DR OMA; HAMRWPD; -.
DR Proteomes; UP000002213; Chromosome.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR Gene3D; 2.40.290.10; -; 1.
DR HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR009187; Prok_Ku.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR PANTHER; PTHR41251; PTHR41251; 1.
DR Pfam; PF02735; Ku; 1.
DR PIRSF; PIRSF006493; Prok_Ku; 1.
DR SMART; SM00559; Ku78; 1.
DR SUPFAM; SSF100939; SSF100939; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
FT CHAIN 1..286
FT /note="Non-homologous end joining protein Ku"
FT /id="PRO_0000389172"
FT DOMAIN 10..175
FT /note="Ku"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT REGION 226..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 226..246
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 286 AA; 31049 MW; D61C240CD38377BC CRC64;
MRATWRGSVT VGLVSFPVRL TPVRQERGVR LHQVHRVDGG RVRHRRVCEL CGEELEAADL
ARGHDSGAGV VLVEDEELAG LRALPSRAVE VVQFSPAGQV DPVLLGRAYY LEPEEPGTGS
YSVLREVLER GGLVGIARVP LRGREVVAVV RPRGGVLVLQ VLVWAEEVRE PDFVVPVAVS
GERELGLAAS LVGAMTEDFD PAAFPDAYSA GLTRVVADKL AGQRLERQER QRREAGEVRQ
ADETDEAAET EVPEVDIPAS RAPGETGGEL LAALRRSLER LRGGRR
