ID   KU_BACHK                Reviewed;         270 AA.
AC   Q6HN08;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN   Name=ku {ECO:0000255|HAMAP-Rule:MF_01875}; OrderedLocusNames=BT9727_0719;
OS   Bacillus thuringiensis subsp. konkukian (strain 97-27).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=281309;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=97-27;
RX   PubMed=16621833; DOI=10.1128/jb.188.9.3382-3390.2006;
RA   Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D.,
RA   Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C.,
RA   Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A.,
RA   Hill K.K., Hitchcock P., Jackson P.J., Keim P., Kewalramani A.R.,
RA   Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M.,
RA   Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B.,
RA   Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R.,
RA   Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L.,
RA   Brettin T.S., Gilna P.;
RT   "Pathogenomic sequence analysis of Bacillus cereus and Bacillus
RT   thuringiensis isolates closely related to Bacillus anthracis.";
RL   J. Bacteriol. 188:3382-3390(2006).
CC   -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC       repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC       linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC       nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC       {ECO:0000255|HAMAP-Rule:MF_01875}.
CC   -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
CC   -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
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DR   EMBL; AE017355; AAT59129.1; -; Genomic_DNA.
DR   RefSeq; WP_000557715.1; NC_005957.1.
DR   RefSeq; YP_035063.1; NC_005957.1.
DR   AlphaFoldDB; Q6HN08; -.
DR   PRIDE; Q6HN08; -.
DR   EnsemblBacteria; AAT59129; AAT59129; BT9727_0719.
DR   KEGG; btk:BT9727_0719; -.
DR   PATRIC; fig|281309.8.peg.753; -.
DR   HOGENOM; CLU_048975_1_0_9; -.
DR   OMA; IRYRKVC; -.
DR   Proteomes; UP000001301; Chromosome.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR   CDD; cd00789; KU_like; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR009187; Prok_Ku.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   PANTHER; PTHR41251; PTHR41251; 1.
DR   Pfam; PF02735; Ku; 1.
DR   PIRSF; PIRSF006493; Prok_Ku; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SSF100939; 1.
DR   TIGRFAMs; TIGR02772; Ku_bact; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; DNA-binding.
FT   CHAIN           1..270
FT                   /note="Non-homologous end joining protein Ku"
FT                   /id="PRO_0000389177"
FT   DOMAIN          10..194
FT                   /note="Ku"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
SQ   SEQUENCE   270 AA;  31210 MW;  0EF2146BE4B1C9C7 CRC64;
     MHTVWKGALS LGLLNIGIKL YSAVDENDIK FLSLHKECLT PIKYKKFAPD CTDEEVDDKD
     IVKAYEYAPH KYIIMDEKEL SALQKAHEPR SIRIISFIQN NEIDSVLYDR SYFIGPTPGH
     EKSYLLLKEA LERTNKLGLI HISIRKKQHL AIIRNFEDGL MLQTIHYPNE IRDITNIPNL
     PSNENYPIQK QELTAAINLI HHLTNPFEQE LYTDEYKEAL TELIENKIEE QAKTESISPA
     PNIINIMETL QASIEQAKIK RDNKTEKEAK