KU_BACSU
ID KU_BACSU Reviewed; 311 AA.
AC O34859;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN Name=ku {ECO:0000255|HAMAP-Rule:MF_01875}; Synonyms=ykoV;
GN OrderedLocusNames=BSU13410;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP PRELIMINARY FUNCTION, AND SIMILARITY.
RX PubMed=11445083; DOI=10.1016/s0014-5793(01)02589-3;
RA Doherty A.J., Jackson S.P., Weller G.R.;
RT "Identification of bacterial homologues of the Ku DNA repair proteins.";
RL FEBS Lett. 500:186-188(2001).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=12215643; DOI=10.1126/science.1074584;
RA Weller G.R., Kysela B., Roy R., Tonkin L.M., Scanlan E., Della M.,
RA Devine S.K., Day J.P., Wilkinson A., d'Adda di Fagagna F., Devine K.M.,
RA Bowater R.P., Jeggo P.A., Jackson S.P., Doherty A.J.;
RT "Identification of a DNA nonhomologous end-joining complex in bacteria.";
RL Science 297:1686-1689(2002).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=168 / PY79;
RX PubMed=16497325; DOI=10.1016/j.jmb.2006.01.059;
RA Wang S.T., Setlow B., Conlon E.M., Lyon J.L., Imamura D., Sato T.,
RA Setlow P., Losick R., Eichenberger P.;
RT "The forespore line of gene expression in Bacillus subtilis.";
RL J. Mol. Biol. 358:16-37(2006).
RN [5]
RP DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=17293412; DOI=10.1128/jb.00018-07;
RA Moeller R., Stackebrandt E., Reitz G., Berger T., Rettberg P.,
RA Doherty A.J., Horneck G., Nicholson W.L.;
RT "Role of DNA repair by nonhomologous-end joining in Bacillus subtilis spore
RT resistance to extreme dryness, mono- and polychromatic UV, and ionizing
RT radiation.";
RL J. Bacteriol. 189:3306-3311(2007).
CC -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC nor ssDNA. Recruits and stimulates the ligase activity of LigD (By
CC similarity). Probably involved in DNA repair during spore germination.
CC {ECO:0000255|HAMAP-Rule:MF_01875, ECO:0000269|PubMed:16497325}.
CC -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC Rule:MF_01875}.
CC -!- SUBCELLULAR LOCATION: Spore core {ECO:0000269|PubMed:16497325}.
CC Note=Expressed in the center of the spore core, presumably with the
CC nucleoid.
CC -!- DEVELOPMENTAL STAGE: Expressed in sporulating cells. Disappears after
CC 90 minutes of spore germination. {ECO:0000269|PubMed:16497325}.
CC -!- INDUCTION: Transcriptionally regulated by SpoVT and sigma-G factor.
CC {ECO:0000269|PubMed:16497325}.
CC -!- DISRUPTION PHENOTYPE: Stationary-phase cells lacking this gene are more
CC sensitive to ionizing radiation. Growth rate, ultraviolet light and
CC methyl-methanesulfonate sensitivities are unaffected. The spores formed
CC are significantly more sensitive to dry heat, high vacuum-induced
CC desiccation, X-rays and UV radiation, but no difference in hydrogen
CC peroxide resistance exists. {ECO:0000269|PubMed:12215643,
CC ECO:0000269|PubMed:16497325, ECO:0000269|PubMed:17293412}.
CC -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC Rule:MF_01875}.
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DR EMBL; AL009126; CAB13198.1; -; Genomic_DNA.
DR PIR; H69860; H69860.
DR RefSeq; NP_389224.1; NC_000964.3.
DR RefSeq; WP_010886496.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; O34859; -.
DR SMR; O34859; -.
DR STRING; 224308.BSU13410; -.
DR PaxDb; O34859; -.
DR PRIDE; O34859; -.
DR DNASU; 939375; -.
DR EnsemblBacteria; CAB13198; CAB13198; BSU_13410.
DR GeneID; 939375; -.
DR KEGG; bsu:BSU13410; -.
DR PATRIC; fig|224308.43.peg.1414; -.
DR eggNOG; COG1273; Bacteria.
DR InParanoid; O34859; -.
DR OMA; IRYRKVC; -.
DR PhylomeDB; O34859; -.
DR BioCyc; BSUB:BSU13410-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR CDD; cd00789; KU_like; 1.
DR Gene3D; 2.40.290.10; -; 1.
DR HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR009187; Prok_Ku.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR PANTHER; PTHR41251; PTHR41251; 1.
DR Pfam; PF02735; Ku; 1.
DR PIRSF; PIRSF006493; Prok_Ku; 1.
DR SMART; SM00559; Ku78; 1.
DR SUPFAM; SSF100939; SSF100939; 1.
DR TIGRFAMs; TIGR02772; Ku_bact; 1.
PE 2: Evidence at transcript level;
KW DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
FT CHAIN 1..311
FT /note="Non-homologous end joining protein Ku"
FT /id="PRO_0000389176"
FT DOMAIN 26..210
FT /note="Ku"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT REGION 269..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 311 AA; 35065 MW; FEEB8B834C59C01E CRC64;
MNRTPSLHTK EKKGFIDMHT MWKGSISFGL VNIPIKLYAA TEDKDIKLRS LHKEDHAPIK
YEKVCTNCEK TLSPDEIVKG YEYVKGKYVV LTDEDLKSLK QEHEEKAVEI VDFVQLQEID
PIYFNRSYFV GPGDNGTKAY TLLREALRST GKIGIANMTI RSKQQLAILR VYENCIVMES
IHYPDEVRSA AQVPGVPDQS NVNDKELQTA ITLIDELTAK FEPEKYEDTY RQALLQRVND
KLENKETAVT PDKAPPREDV IDLVSALQAS IDRTRRPNRE TPAAAPAQAA EPKGAGDKKQ
KTTRKKASGT S
