KU_BRASO
ID KU_BRASO Reviewed; 292 AA.
AC A4Z012;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN Name=ku {ECO:0000255|HAMAP-Rule:MF_01875}; OrderedLocusNames=BRADO5824;
OS Bradyrhizobium sp. (strain ORS 278).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium.
OX NCBI_TaxID=114615;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ORS 278;
RX PubMed=17540897; DOI=10.1126/science.1139548;
RA Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C.,
RA Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S., Rouy Z.,
RA Mangenot S., Segurens B., Dossat C., Franck W.L., Chang W.-S., Saunders E.,
RA Bruce D., Richardson P., Normand P., Dreyfus B., Pignol D., Stacey G.,
RA Emerich D., Vermeglio A., Medigue C., Sadowsky M.;
RT "Legumes symbioses: absence of nod genes in photosynthetic bradyrhizobia.";
RL Science 316:1307-1312(2007).
CC -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC {ECO:0000255|HAMAP-Rule:MF_01875}.
CC -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC Rule:MF_01875}.
CC -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC Rule:MF_01875}.
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DR EMBL; CU234118; CAL79488.1; -; Genomic_DNA.
DR RefSeq; WP_012029391.1; NC_009445.1.
DR AlphaFoldDB; A4Z012; -.
DR SMR; A4Z012; -.
DR STRING; 114615.BRADO5824; -.
DR EnsemblBacteria; CAL79488; CAL79488; BRADO5824.
DR KEGG; bra:BRADO5824; -.
DR eggNOG; COG1273; Bacteria.
DR HOGENOM; CLU_048975_0_0_5; -.
DR OMA; HAMRWPD; -.
DR OrthoDB; 1724780at2; -.
DR BioCyc; BSP114615:BRADO_RS27105-MON; -.
DR Proteomes; UP000001994; Chromosome.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR CDD; cd00789; KU_like; 1.
DR Gene3D; 2.40.290.10; -; 1.
DR HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR009187; Prok_Ku.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR PANTHER; PTHR41251; PTHR41251; 1.
DR Pfam; PF02735; Ku; 1.
DR PIRSF; PIRSF006493; Prok_Ku; 1.
DR SMART; SM00559; Ku78; 1.
DR SUPFAM; SSF100939; SSF100939; 1.
DR TIGRFAMs; TIGR02772; Ku_bact; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
FT CHAIN 1..292
FT /note="Non-homologous end joining protein Ku"
FT /id="PRO_0000389178"
FT DOMAIN 12..196
FT /note="Ku"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT REGION 231..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 268..292
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 292 AA; 32748 MW; 17ECF4E1C57BFCC8 CRC64;
MAPRAYWKGS LKLSLVTCPV VLYPASTSVE KTRFHLINRE TGNRLKQQMI DAETGDLVEG
DQKGRGYELS KGQYVEIEPE ELEAVQIESN HTIDIDSFVP REEIDQRYLN HPYYIAPDGK
AAVDAFAVIR DAMKDQDRVA LARIVLTHRE HVIAIEPMGK GMLGTTLRFP YELRDEAEFF
DDIKAPKITK DMVELAGHIL QTKAAHFKPS EFKDQYETAL KALVKRKASG KPIKLPEPEE
RSGNVVSLMD ALKQSLGKGD KKAAPAPSRR APAHRRPAKK AHRSAARQRK AG
