ID   KU_FLAJ1                Reviewed;         259 AA.
AC   A5FEP6;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN   Name=ku {ECO:0000255|HAMAP-Rule:MF_01875}; OrderedLocusNames=Fjoh_3304;
OS   Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC
OS   14942 / NCIMB 11054 / UW101) (Cytophaga johnsonae).
OC   Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae; Flavobacterium.
OX   NCBI_TaxID=376686;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101;
RX   PubMed=19717629; DOI=10.1128/aem.01495-09;
RA   McBride M.J., Xie G., Martens E.C., Lapidus A., Henrissat B., Rhodes R.G.,
RA   Goltsman E., Wang W., Xu J., Hunnicutt D.W., Staroscik A.M., Hoover T.R.,
RA   Cheng Y.Q., Stein J.L.;
RT   "Novel features of the polysaccharide-digesting gliding bacterium
RT   Flavobacterium johnsoniae as revealed by genome sequence analysis.";
RL   Appl. Environ. Microbiol. 75:6864-6875(2009).
CC   -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC       repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC       linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC       nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC       {ECO:0000255|HAMAP-Rule:MF_01875}.
CC   -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
CC   -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
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DR   EMBL; CP000685; ABQ06320.1; -; Genomic_DNA.
DR   RefSeq; WP_012025289.1; NZ_MUGZ01000015.1.
DR   AlphaFoldDB; A5FEP6; -.
DR   SMR; A5FEP6; -.
DR   STRING; 376686.Fjoh_3304; -.
DR   EnsemblBacteria; ABQ06320; ABQ06320; Fjoh_3304.
DR   KEGG; fjo:Fjoh_3304; -.
DR   eggNOG; COG1273; Bacteria.
DR   HOGENOM; CLU_048975_0_1_10; -.
DR   OMA; IRYRKVC; -.
DR   OrthoDB; 1724780at2; -.
DR   Proteomes; UP000006694; Chromosome.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR   CDD; cd00789; KU_like; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR009187; Prok_Ku.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   PANTHER; PTHR41251; PTHR41251; 1.
DR   Pfam; PF02735; Ku; 1.
DR   PIRSF; PIRSF006493; Prok_Ku; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SSF100939; 1.
DR   TIGRFAMs; TIGR02772; Ku_bact; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; DNA-binding.
FT   CHAIN           1..259
FT                   /note="Non-homologous end joining protein Ku"
FT                   /id="PRO_0000389183"
FT   DOMAIN          9..187
FT                   /note="Ku"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
SQ   SEQUENCE   259 AA;  29558 MW;  835CAE682E126B0E CRC64;
     MRSIWTGSVS FGLINIPIKM FSAVQESSLD MDMLDKKDNA NIKFKRVNEN TGKEVDFANI
     VKGYMLNDKY VILDDSDFEA ADAIKTKTID IESFVLEKEI ESIYYEQPYY LEPDKGAMNA
     YGLLRDALQA SGKVGVTRFV LRNKESLAIL KPYKNVIVLN RIRFEQEIRS TDELNLPPIS
     KKATKEMDMA EKLIDQLTEK FDITGFKDEY TTKLLDIIKK KAKGKVQKTA PKMKVVHKQG
     DDLMSMLKAS LETKKKKSS