ID   KU_GLUDA                Reviewed;         268 AA.
AC   A9H271;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN   Name=ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN   OrderedLocusNames=GDI0170, Gdia_2240;
OS   Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / CCUG 37298
OS   / CIP 103539 / LMG 7603 / PAl5).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Acetobacteraceae; Gluconacetobacter.
OX   NCBI_TaxID=272568;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49037 / DSM 5601 / CCUG 37298 / CIP 103539 / LMG 7603 / PAl5;
RX   PubMed=19775431; DOI=10.1186/1471-2164-10-450;
RA   Bertalan M., Albano R., de Padua V., Rouws L., Rojas C., Hemerly A.,
RA   Teixeira K., Schwab S., Araujo J., Oliveira A., Franca L., Magalhaes V.,
RA   Alqueres S., Cardoso A., Almeida W., Loureiro M.M., Nogueira E., Cidade D.,
RA   Oliveira D., Simao T., Macedo J., Valadao A., Dreschsel M., Freitas F.,
RA   Vidal M., Guedes H., Rodrigues E., Meneses C., Brioso P., Pozzer L.,
RA   Figueiredo D., Montano H., Junior J., de Souza Filho G.,
RA   Martin Quintana Flores V., Ferreira B., Branco A., Gonzalez P.,
RA   Guillobel H., Lemos M., Seibel L., Macedo J., Alves-Ferreira M.,
RA   Sachetto-Martins G., Coelho A., Santos E., Amaral G., Neves A.,
RA   Pacheco A.B., Carvalho D., Lery L., Bisch P., Rossle S.C., Urmenyi T.,
RA   Rael Pereira A., Silva R., Rondinelli E., von Kruger W., Martins O.,
RA   Baldani J.I., Ferreira P.C.;
RT   "Complete genome sequence of the sugarcane nitrogen-fixing endophyte
RT   Gluconacetobacter diazotrophicus Pal5.";
RL   BMC Genomics 10:450-450(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49037 / DSM 5601 / CCUG 37298 / CIP 103539 / LMG 7603 / PAl5;
RX   PubMed=21304715; DOI=10.4056/sigs.972221;
RA   Giongo A., Tyler H.L., Zipperer U.N., Triplett E.W.;
RT   "Two genome sequences of the same bacterial strain, Gluconacetobacter
RT   diazotrophicus PAl 5, suggest a new standard in genome sequence
RT   submission.";
RL   Stand. Genomic Sci. 2:309-317(2010).
CC   -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC       repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC       linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC       nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC       {ECO:0000255|HAMAP-Rule:MF_01875}.
CC   -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
CC   -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
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DR   EMBL; CP001189; ACI51995.1; -; Genomic_DNA.
DR   EMBL; AM889285; CAP54113.1; -; Genomic_DNA.
DR   RefSeq; WP_012222409.1; NC_011365.1.
DR   AlphaFoldDB; A9H271; -.
DR   SMR; A9H271; -.
DR   STRING; 272568.GDI0170; -.
DR   EnsemblBacteria; ACI51995; ACI51995; Gdia_2240.
DR   EnsemblBacteria; CAP54113; CAP54113; GDI0170.
DR   KEGG; gdi:GDI0170; -.
DR   KEGG; gdj:Gdia_2240; -.
DR   eggNOG; COG1273; Bacteria.
DR   HOGENOM; CLU_048975_0_0_5; -.
DR   OMA; QVKGYPR; -.
DR   OrthoDB; 1724780at2; -.
DR   Proteomes; UP000000736; Chromosome.
DR   Proteomes; UP000001176; Chromosome.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR   CDD; cd00789; KU_like; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR009187; Prok_Ku.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   PANTHER; PTHR41251; PTHR41251; 1.
DR   Pfam; PF02735; Ku; 1.
DR   PIRSF; PIRSF006493; Prok_Ku; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SSF100939; 1.
DR   TIGRFAMs; TIGR02772; Ku_bact; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
FT   CHAIN           1..268
FT                   /note="Non-homologous end joining protein Ku"
FT                   /id="PRO_0000389187"
FT   DOMAIN          13..175
FT                   /note="Ku"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT   REGION          174..194
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   268 AA;  29463 MW;  0910C13D15E34D29 CRC64;
     MADRPSWTGY LKVSLVTCPV TMMPATTGSE TVRFHTINRR TGHRVVSRYV DAQSGAPVAD
     DEQVLGYPRD DDHYVLLEDE ELESVALDST RTIDIESFVP AGSIGWVWYD APHFLMPDDT
     IGVEAFAVIR AAMEAAGMVG IARLVLYRRE RAVLLEPRGK GIVLWTLHDG NAVRNGGHPA
     ARTRPASEAE SADSPEMKLL TALIDRQTQP WDPEMVRDPV QERLLDIIAA RQKGRKRAPA
     REAAKPKTGN VVSILDALRA SLAQDGTS