KU_HELMI
ID KU_HELMI Reviewed; 276 AA.
AC B0TIF2;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-NOV-2009, sequence version 2.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN Name=ku {ECO:0000255|HAMAP-Rule:MF_01875}; OrderedLocusNames=Helmi_22680;
GN ORFNames=HM1_3135;
OS Heliobacterium modesticaldum (strain ATCC 51547 / Ice1).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Heliobacteriaceae;
OC Heliomicrobium.
OX NCBI_TaxID=498761;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51547 / Ice1;
RX PubMed=18441057; DOI=10.1128/jb.00299-08;
RA Sattley W.M., Madigan M.T., Swingley W.D., Cheung P.C., Clocksin K.M.,
RA Conrad A.L., Dejesa L.C., Honchak B.M., Jung D.O., Karbach L.E.,
RA Kurdoglu A., Lahiri S., Mastrian S.D., Page L.E., Taylor H.L., Wang Z.T.,
RA Raymond J., Chen M., Blankenship R.E., Touchman J.W.;
RT "The genome of Heliobacterium modesticaldum, a phototrophic representative
RT of the Firmicutes containing the simplest photosynthetic apparatus.";
RL J. Bacteriol. 190:4687-4696(2008).
CC -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC {ECO:0000255|HAMAP-Rule:MF_01875}.
CC -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC Rule:MF_01875}.
CC -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC Rule:MF_01875}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABZ84893.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP000930; ABZ84893.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; B0TIF2; -.
DR SMR; B0TIF2; -.
DR STRING; 498761.HM1_3135; -.
DR EnsemblBacteria; ABZ84893; ABZ84893; HM1_3135.
DR KEGG; hmo:HM1_3135; -.
DR eggNOG; COG1273; Bacteria.
DR HOGENOM; CLU_048975_1_0_9; -.
DR Proteomes; UP000008550; Chromosome.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR CDD; cd00789; KU_like; 1.
DR Gene3D; 2.40.290.10; -; 1.
DR HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR009187; Prok_Ku.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR PANTHER; PTHR41251; PTHR41251; 1.
DR Pfam; PF02735; Ku; 1.
DR PIRSF; PIRSF006493; Prok_Ku; 1.
DR SMART; SM00559; Ku78; 1.
DR SUPFAM; SSF100939; SSF100939; 1.
DR TIGRFAMs; TIGR02772; Ku_bact; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
FT CHAIN 1..276
FT /note="Non-homologous end joining protein Ku"
FT /id="PRO_0000389188"
FT DOMAIN 11..177
FT /note="Ku"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT REGION 256..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 276 AA; 31643 MW; 115E070B976E7603 CRC64;
MIVRSIWKGA ISFGLVHIPI KLFAATEEKD VRFHLLHKEC HNPIQYQKRC PYCDREVTPE
EIVKGFEYDK GRYVIITQEE LEGLAPEGSR SIDIQSFVAL KEIDPIFFVK TYYLSPDQHG
QKAYALLRNA LRETDRLALA RVILRTKEAL VALRVYGKGL AMHTMLYPEE IRSMEPLGDL
GESIEVSAKE QTMAVQLIES LTEPFDPVKW QSEQRERIRH FINAKVQGQA IVEAPQTPTV
GKVIDLMEAL KASIQQVKTQ QKKEAAPKKE RRRKTS
