ID   KU_METSB                Reviewed;         271 AA.
AC   B8EKE1;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN   Name=ku {ECO:0000255|HAMAP-Rule:MF_01875}; OrderedLocusNames=Msil_1735;
OS   Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB
OS   13906 / BL2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Beijerinckiaceae; Methylocella.
OX   NCBI_TaxID=395965;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2;
RX   PubMed=20472789; DOI=10.1128/jb.00506-10;
RA   Chen Y., Crombie A., Rahman M.T., Dedysh S.N., Liesack W., Stott M.B.,
RA   Alam M., Theisen A.R., Murrell J.C., Dunfield P.F.;
RT   "Complete genome sequence of the aerobic facultative methanotroph
RT   Methylocella silvestris BL2.";
RL   J. Bacteriol. 192:3840-3841(2010).
CC   -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC       repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC       linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC       nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC       {ECO:0000255|HAMAP-Rule:MF_01875}.
CC   -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
CC   -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
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DR   EMBL; CP001280; ACK50681.1; -; Genomic_DNA.
DR   RefSeq; WP_012590751.1; NC_011666.1.
DR   AlphaFoldDB; B8EKE1; -.
DR   SMR; B8EKE1; -.
DR   STRING; 395965.Msil_1735; -.
DR   EnsemblBacteria; ACK50681; ACK50681; Msil_1735.
DR   KEGG; msl:Msil_1735; -.
DR   eggNOG; COG1273; Bacteria.
DR   HOGENOM; CLU_048975_0_0_5; -.
DR   OMA; QVKGYPR; -.
DR   OrthoDB; 1724780at2; -.
DR   Proteomes; UP000002257; Chromosome.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.290.10; -; 1.
DR   HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR009187; Prok_Ku.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   PANTHER; PTHR41251; PTHR41251; 1.
DR   Pfam; PF02735; Ku; 1.
DR   PIRSF; PIRSF006493; Prok_Ku; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SSF100939; 1.
DR   TIGRFAMs; TIGR02772; Ku_bact; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
FT   CHAIN           1..271
FT                   /note="Non-homologous end joining protein Ku"
FT                   /id="PRO_0000389190"
FT   DOMAIN          12..194
FT                   /note="Ku"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT   REGION          225..249
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        225..245
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   271 AA;  30596 MW;  553471B25CB08D53 CRC64;
     MAPRSFWKGY LKLSLVTCPV VMAPAKSEKE KLRFHTLNRA TGNRVQSRYI DSVSGKPVDS
     ADQVKGFPRD DQSYVMLEDE ELDSVALESA RTIDIELFAP ADSIDWIWYD APHYLTPGDE
     VGEEAFAVIR EAMKATKMVG ISRLVLYRRE RAVLLEPRGK GIILWTLRYG DEVRDPKLYF
     DAIKDQKPVP ELLSLVNRLI EERVKPWSPE MASDPVQDRL RDIIEAKKTP PAKKTKAEEK
     TGKGSAESNV IDIMDALRKS LGPAAKKPKG R