KU_MYCS2
ID KU_MYCS2 Reviewed; 318 AA.
AC A0R3S7; I7G886;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 19-MAR-2014, sequence version 2.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN Name=ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN OrderedLocusNames=MSMEG_5580, MSMEI_5431;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=15778718; DOI=10.1038/nsmb915;
RA Gong C., Bongiorno P., Martins A., Stephanou N.C., Zhu H., Shuman S.,
RA Glickman M.S.;
RT "Mechanism of nonhomologous end-joining in mycobacteria: a low-fidelity
RT repair system driven by Ku, ligase D and ligase C.";
RL Nat. Struct. Mol. Biol. 12:304-312(2005).
RN [5]
RP FUNCTION.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=16949369; DOI=10.1016/j.molcel.2006.07.009;
RA Pitcher R.S., Tonkin L.M., Daley J.M., Palmbos P.L., Green A.J.,
RA Velting T.L., Brzostek A., Korycka-Machala M., Cresawn S., Dziadek J.,
RA Hatfull G.F., Wilson T.E., Doherty A.J.;
RT "Mycobacteriophage exploit NHEJ to facilitate genome circularization.";
RL Mol. Cell 23:743-748(2006).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17360246; DOI=10.1016/j.dnarep.2007.02.009;
RA Pitcher R.S., Green A.J., Brzostek A., Korycka-Machala M., Dziadek J.,
RA Doherty A.J.;
RT "NHEJ protects mycobacteria in stationary phase against the harmful effects
RT of desiccation.";
RL DNA Repair 6:1271-1276(2007).
RN [7]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17496093; DOI=10.1128/jb.00332-07;
RA Stephanou N.C., Gao F., Bongiorno P., Ehrt S., Schnappinger D., Shuman S.,
RA Glickman M.S.;
RT "Mycobacterial nonhomologous end joining mediates mutagenic repair of
RT chromosomal double-strand DNA breaks.";
RL J. Bacteriol. 189:5237-5246(2007).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18281464; DOI=10.1101/gad.1631908;
RA Aniukwu J., Glickman M.S., Shuman S.;
RT "The pathways and outcomes of mycobacterial NHEJ depend on the structure of
RT the broken DNA ends.";
RL Genes Dev. 22:512-527(2008).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=21219454; DOI=10.1111/j.1365-2958.2010.07463.x;
RA Gupta R., Barkan D., Redelman-Sidi G., Shuman S., Glickman M.S.;
RT "Mycobacteria exploit three genetically distinct DNA double-strand break
RT repair pathways.";
RL Mol. Microbiol. 79:316-330(2011).
RN [10]
RP FUNCTION, INTERACTION WITH SIR2, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=21637345; DOI=10.1371/journal.pone.0020045;
RA Li Z., Wen J., Lin Y., Wang S., Xue P., Zhang Z., Zhou Y., Wang X., Sui L.,
RA Bi L.J., Zhang X.E.;
RT "A Sir2-like protein participates in mycobacterial NHEJ.";
RL PLoS ONE 6:E20045-E20045(2011).
CC -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) repair
CC enzyme which repairs blunt-end and 5'-overhang double strand breaks
CC (DSB) with about 50% fidelity, and DSB with non-complementary 3' ends.
CC Plays a partial role in NHEJ on 3'-overhang repair of complementary
CC ends. NHEJ repairs DSB with blunt ends and 5' overhangs with a high
CC level of nucleotide insertion/deletion, without a need for
CC microhomology. This protein but not LigD also suppresses homologous
CC recombination. Overexpression dramatically increases the efficiency of
CC NHEJ with no effect on repair fidelity. {ECO:0000269|PubMed:15778718,
CC ECO:0000269|PubMed:16949369, ECO:0000269|PubMed:17360246,
CC ECO:0000269|PubMed:18281464, ECO:0000269|PubMed:21219454,
CC ECO:0000269|PubMed:21637345}.
CC -!- SUBUNIT: Homodimer. Interacts with Sir2 and probably also with LigD;
CC may form a trimeric complex during NHEJ. {ECO:0000269|PubMed:21637345}.
CC -!- DISRUPTION PHENOTYPE: Not essential for growth in the absence of DNA
CC damage. 500-fold less efficient for NHEJ on blunt-ended or 5'overhang
CC DSBs, 4-fold less efficient in repair of 3'-overhang DSBs. The fidelity
CC of DNA repair depends on the form of the DSB; for blunt-ends fidelity
CC is very low, and no longer entails nucleotide insertion, for 5'-
CC overhangs remains 50% faithful but with very little nucleotide
CC insertion, for 3'-overhangs repair is fully faithful. 1000-fold
CC decrease in viability when exposed to ionizing radiation in late and
CC stationary phase; not exacerbated by a double ligD-ku deletion.
CC Decreased resistance to desiccation-induced DSBs. Loss of NHEJ on
CC incompatible 3'-chromosomal overhangs, no effect on single-strand
CC annealing of DSB repair, increase in homologous recombination.
CC {ECO:0000269|PubMed:15778718, ECO:0000269|PubMed:17360246,
CC ECO:0000269|PubMed:17496093, ECO:0000269|PubMed:18281464,
CC ECO:0000269|PubMed:21219454, ECO:0000269|PubMed:21637345}.
CC -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC Rule:MF_01875}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK73702.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP000480; ABK73702.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP001663; AFP41872.1; -; Genomic_DNA.
DR RefSeq; WP_014878394.1; NZ_SIJM01000006.1.
DR RefSeq; YP_889815.1; NC_008596.1.
DR AlphaFoldDB; A0R3S7; -.
DR SMR; A0R3S7; -.
DR STRING; 246196.MSMEI_5431; -.
DR EnsemblBacteria; ABK73702; ABK73702; MSMEG_5580.
DR EnsemblBacteria; AFP41872; AFP41872; MSMEI_5431.
DR GeneID; 66736878; -.
DR KEGG; msg:MSMEI_5431; -.
DR KEGG; msm:MSMEG_5580; -.
DR PATRIC; fig|246196.19.peg.5441; -.
DR eggNOG; COG1273; Bacteria.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IMP:UniProtKB.
DR CDD; cd00789; KU_like; 1.
DR Gene3D; 2.40.290.10; -; 1.
DR HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR009187; Prok_Ku.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR PANTHER; PTHR41251; PTHR41251; 1.
DR Pfam; PF02735; Ku; 1.
DR PIRSF; PIRSF006493; Prok_Ku; 1.
DR SMART; SM00559; Ku78; 1.
DR SUPFAM; SSF100939; SSF100939; 1.
DR TIGRFAMs; TIGR02772; Ku_bact; 1.
PE 1: Evidence at protein level;
KW DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
FT CHAIN 1..318
FT /note="Non-homologous end joining protein Ku"
FT /id="PRO_0000425944"
FT DOMAIN 10..193
FT /note="Ku"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT REGION 259..318
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 259..293
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..318
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 318 AA; 35712 MW; D76849B995FA6C29 CRC64;
MRSIWKGSIA FGLVNVPVKV YSATEDHDIK FHQVHAKDNG RIRYKRVCEV CGEVVEYRDI
NKAFESDDGQ MVVITDEDIA TLPEERSREI EVVEFIPAEQ LDPLMYDKSY FLEPDSKSSK
SYVLLAKTLA ETDRIAIVHF SLRNKSRLAA LRVKDFSKRD VMMIHTLLWP DEIRDPDFPI
LDKEVQIKPA ELKMAGQVVE SMTDDFKPDL YHDDYQEQLR ELVQAKLEGG EAFSVEEQPA
ELDEGTEDVS DLLAKLEASV KARKGGKSDS KDDSDSESDS KESKSDSKPA KKAPAKKAAA
KKSTAKKAPA KKAAAKKS
