ID   KU_MYCTO                Reviewed;         273 AA.
AC   P9WKD8; L7N5V9;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
DE   AltName: Full=Mt-Ku;
GN   Name=mku; OrderedLocusNames=MT0964;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC       repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC       linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC       nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC       {ECO:0000255|HAMAP-Rule:MF_01875}.
CC   -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
CC   -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
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DR   EMBL; AE000516; AAK45211.1; -; Genomic_DNA.
DR   PIR; A70585; A70585.
DR   RefSeq; WP_003404795.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WKD8; -.
DR   SMR; P9WKD8; -.
DR   EnsemblBacteria; AAK45211; AAK45211; MT0964.
DR   KEGG; mtc:MT0964; -.
DR   PATRIC; fig|83331.31.peg.1034; -.
DR   HOGENOM; CLU_048975_1_1_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR   CDD; cd00789; KU_like; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR009187; Prok_Ku.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   PANTHER; PTHR41251; PTHR41251; 1.
DR   Pfam; PF02735; Ku; 1.
DR   PIRSF; PIRSF006493; Prok_Ku; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SSF100939; 1.
DR   TIGRFAMs; TIGR02772; Ku_bact; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; DNA-binding.
FT   CHAIN           1..273
FT                   /note="Non-homologous end joining protein Ku"
FT                   /id="PRO_0000427671"
FT   DOMAIN          10..193
FT                   /note="Ku"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT   REGION          111..273
FT                   /note="Sufficient for interaction with LigD"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   273 AA;  30904 MW;  DE11166BC1C52519 CRC64;
     MRAIWTGSIA FGLVNVPVKV YSATADHDIR FHQVHAKDNG RIRYKRVCEA CGEVVDYRDL
     ARAYESGDGQ MVAITDDDIA SLPEERSREI EVLEFVPAAD VDPMMFDRSY FLEPDSKSSK
     SYVLLAKTLA ETDRMAIVHF TLRNKTRLAA LRVKDFGKRE VMMVHTLLWP DEIRDPDFPV
     LDQKVEIKPA ELKMAGQVVD SMADDFNPDR YHDTYQEQLQ ELIDTKLEGG QAFTAEDQPR
     LLDEPEDVSD LLAKLEASVK ARSKANSNVP TPP