ID   KU_PSEAE                Reviewed;         293 AA.
AC   Q9I1W5;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN   Name=ku {ECO:0000255|HAMAP-Rule:MF_01875}; OrderedLocusNames=PA2150;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, SUBUNIT, AND DNA-BINDING.
RX   PubMed=20018881; DOI=10.1074/jbc.m109.073874;
RA   Zhu H., Shuman S.;
RT   "Gap filling activities of Pseudomonas DNA ligase D (LigD) polymerase and
RT   functional interactions of LigD with the DNA end-binding Ku protein.";
RL   J. Biol. Chem. 285:4815-4825(2010).
CC   -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC       repair enzyme, which repairs dsDNA breaks with reduced fidelity.
CC       Stimulates rNTP addition to DSB and end joining (ligation) of linear
CC       DNA by LigD, on 3'-overhangs and probably also 5'-overhangs and blunt
CC       dsDNA breaks. Binds both ends of linear dsDNA protecting it from
CC       exonuclease activity. {ECO:0000269|PubMed:20018881}.
CC   -!- SUBUNIT: Homodimer, may form higher-order multimers on DNA. Non-
CC       dimerized protein does not stimulate LigD ligase activity. Probably
CC       interacts with LigD. {ECO:0000269|PubMed:20018881}.
CC   -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
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DR   EMBL; AE004091; AAG05538.1; -; Genomic_DNA.
DR   PIR; E83376; E83376.
DR   RefSeq; NP_250840.1; NC_002516.2.
DR   RefSeq; WP_003113644.1; NZ_QZGE01000014.1.
DR   AlphaFoldDB; Q9I1W5; -.
DR   SMR; Q9I1W5; -.
DR   STRING; 287.DR97_6276; -.
DR   PaxDb; Q9I1W5; -.
DR   PRIDE; Q9I1W5; -.
DR   EnsemblBacteria; AAG05538; AAG05538; PA2150.
DR   GeneID; 881375; -.
DR   KEGG; pae:PA2150; -.
DR   PATRIC; fig|208964.12.peg.2249; -.
DR   PseudoCAP; PA2150; -.
DR   HOGENOM; CLU_048975_2_0_6; -.
DR   InParanoid; Q9I1W5; -.
DR   OMA; IRYRKVC; -.
DR   PhylomeDB; Q9I1W5; -.
DR   BioCyc; PAER208964:G1FZ6-2190-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0003690; F:double-stranded DNA binding; IDA:UniProtKB.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR   GO; GO:0051351; P:positive regulation of ligase activity; IDA:UniProtKB.
DR   CDD; cd00789; KU_like; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR009187; Prok_Ku.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   PANTHER; PTHR41251; PTHR41251; 1.
DR   Pfam; PF02735; Ku; 1.
DR   PIRSF; PIRSF006493; Prok_Ku; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SSF100939; 1.
DR   TIGRFAMs; TIGR02772; Ku_bact; 1.
PE   1: Evidence at protein level;
KW   DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
FT   CHAIN           1..293
FT                   /note="Non-homologous end joining protein Ku"
FT                   /id="PRO_0000425946"
FT   DOMAIN          10..195
FT                   /note="Ku"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT   REGION          216..229
FT                   /note="Required for dimerization"
FT   REGION          260..293
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        260..279
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   293 AA;  32790 MW;  042CD9E32F8A8041 CRC64;
     MARAIWKGAI SFGLVHIPVS LSAATSSQGI DFDWLDQRSM EPVGYKRVNK VTGKEIEREN
     IVKGVEYEKG RYVVLSEEEI RAAHPKSTQT IEIFAFVDSQ EIPLQHFDTP YYLVPDRRGG
     KVYALLRETL ERTGKVALAN VVLHTRQHLA LLRPLQDALV LITLRWPSQV RSLDGLELDE
     SVTEAKLDKR ELEMAKRLVE DMASHWEPDE YKDSFSDKIM KLVEEKAAKG QLHAVEEEEE
     VAGKGADIID LTDLLKRSLR SRAGGGKDKG SEKAGADAKG RAKSGASRSR RKA