ID   KU_STRAW                Reviewed;         319 AA.
AC   Q82PM3;
DT   24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Non-homologous end joining protein Ku {ECO:0000255|HAMAP-Rule:MF_01875};
GN   Name=ku {ECO:0000255|HAMAP-Rule:MF_01875}; OrderedLocusNames=SAV_879;
OS   Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC
OS   14893 / NCIMB 12804 / NRRL 8165 / MA-4680).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=227882;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL
RC   8165 / MA-4680;
RX   PubMed=12692562; DOI=10.1038/nbt820;
RA   Ikeda H., Ishikawa J., Hanamoto A., Shinose M., Kikuchi H., Shiba T.,
RA   Sakaki Y., Hattori M., Omura S.;
RT   "Complete genome sequence and comparative analysis of the industrial
RT   microorganism Streptomyces avermitilis.";
RL   Nat. Biotechnol. 21:526-531(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL
RC   8165 / MA-4680;
RX   PubMed=11572948; DOI=10.1073/pnas.211433198;
RA   Omura S., Ikeda H., Ishikawa J., Hanamoto A., Takahashi C., Shinose M.,
RA   Takahashi Y., Horikawa H., Nakazawa H., Osonoe T., Kikuchi H., Shiba T.,
RA   Sakaki Y., Hattori M.;
RT   "Genome sequence of an industrial microorganism Streptomyces avermitilis:
RT   deducing the ability of producing secondary metabolites.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:12215-12220(2001).
CC   -!- FUNCTION: With LigD forms a non-homologous end joining (NHEJ) DNA
CC       repair enzyme, which repairs dsDNA breaks with reduced fidelity. Binds
CC       linear dsDNA with 5'- and 3'- overhangs but not closed circular dsDNA
CC       nor ssDNA. Recruits and stimulates the ligase activity of LigD.
CC       {ECO:0000255|HAMAP-Rule:MF_01875}.
CC   -!- SUBUNIT: Homodimer. Interacts with LigD. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
CC   -!- SIMILARITY: Belongs to the prokaryotic Ku family. {ECO:0000255|HAMAP-
CC       Rule:MF_01875}.
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DR   EMBL; BA000030; BAC68589.1; -; Genomic_DNA.
DR   RefSeq; WP_010982317.1; NZ_JZJK01000088.1.
DR   AlphaFoldDB; Q82PM3; -.
DR   SMR; Q82PM3; -.
DR   STRING; 227882.SAV_879; -.
DR   PRIDE; Q82PM3; -.
DR   EnsemblBacteria; BAC68589; BAC68589; SAVERM_879.
DR   KEGG; sma:SAVERM_879; -.
DR   eggNOG; COG1273; Bacteria.
DR   HOGENOM; CLU_048975_1_2_11; -.
DR   OMA; HAMRWPD; -.
DR   OrthoDB; 1724780at2; -.
DR   Proteomes; UP000000428; Chromosome.
DR   GO; GO:0003690; F:double-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:UniProtKB-UniRule.
DR   CDD; cd00789; KU_like; 1.
DR   Gene3D; 2.40.290.10; -; 1.
DR   HAMAP; MF_01875; Prokaryotic_Ku; 1.
DR   InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR   InterPro; IPR009187; Prok_Ku.
DR   InterPro; IPR016194; SPOC-like_C_dom_sf.
DR   PANTHER; PTHR41251; PTHR41251; 1.
DR   Pfam; PF02735; Ku; 1.
DR   PIRSF; PIRSF006493; Prok_Ku; 1.
DR   SMART; SM00559; Ku78; 1.
DR   SUPFAM; SSF100939; SSF100939; 1.
DR   TIGRFAMs; TIGR02772; Ku_bact; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; DNA-binding; Reference proteome.
FT   CHAIN           1..319
FT                   /note="Non-homologous end joining protein Ku"
FT                   /id="PRO_0000389199"
FT   DOMAIN          10..188
FT                   /note="Ku"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01875"
FT   REGION          252..319
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        261..278
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        293..307
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   319 AA;  35798 MW;  CA54AEC56EFA7111 CRC64;
     MPRTIWSGAI SFGLVTVPIH VVSATEDHSV RFHQYHLEDM GRVRVRKYCE LEDREVTQDE
     IGKGYELSKD TVIPVLDEEL RELPLPTAKA IEIEAFVPLA SIDPMGIGEG YYLQPDGQVA
     AKPYKLLRQA LERSSKVAVA KYAWSGRERL GMLRVREEAI VLHAMRWPDE IRDPSELAPQ
     GIELSEDEIT EAEQLIDRLT RDDLEGEEFQ DHYTEALHEV IEAKQEGHAP PEARETEEEP
     GKVLDLMAAL RQSVAKAKAS RGESGEADVH ELPRKKTAAR KTAKQQPAKK TSAKRTAAKE
     PTKKTAAKKT TPKKPRRSA