ID   ARCA_STRPN              Reviewed;         409 AA.
AC   Q97NA4;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Arginine deiminase {ECO:0000255|HAMAP-Rule:MF_00242};
DE            Short=ADI {ECO:0000255|HAMAP-Rule:MF_00242};
DE            EC=3.5.3.6 {ECO:0000255|HAMAP-Rule:MF_00242};
DE   AltName: Full=Arginine dihydrolase {ECO:0000255|HAMAP-Rule:MF_00242};
DE            Short=AD {ECO:0000255|HAMAP-Rule:MF_00242};
GN   Name=arcA {ECO:0000255|HAMAP-Rule:MF_00242}; OrderedLocusNames=SP_2148;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-arginine = L-citrulline + NH4(+);
CC         Xref=Rhea:RHEA:19597, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:32682, ChEBI:CHEBI:57743; EC=3.5.3.6;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00242};
CC   -!- PATHWAY: Amino-acid degradation; L-arginine degradation via ADI
CC       pathway; carbamoyl phosphate from L-arginine: step 1/2.
CC       {ECO:0000255|HAMAP-Rule:MF_00242}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00242}.
CC   -!- SIMILARITY: Belongs to the arginine deiminase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00242}.
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DR   EMBL; AE005672; AAK76205.1; -; Genomic_DNA.
DR   PIR; D95251; D95251.
DR   RefSeq; WP_000094620.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; Q97NA4; -.
DR   SMR; Q97NA4; -.
DR   STRING; 170187.SP_2148; -.
DR   EnsemblBacteria; AAK76205; AAK76205; SP_2148.
DR   KEGG; spn:SP_2148; -.
DR   eggNOG; COG2235; Bacteria.
DR   OMA; ERATMHL; -.
DR   PhylomeDB; Q97NA4; -.
DR   BioCyc; SPNE170187:G1FZB-2240-MON; -.
DR   UniPathway; UPA00254; UER00364.
DR   PHI-base; PHI:3473; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016990; F:arginine deiminase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019547; P:arginine catabolic process to ornithine; IEA:UniProtKB-UniPathway.
DR   GO; GO:0018101; P:protein citrullination; IEA:GOC.
DR   HAMAP; MF_00242; Arg_deiminase; 1.
DR   InterPro; IPR003876; Arg_deiminase.
DR   PIRSF; PIRSF006356; Arg_deiminase; 1.
DR   PRINTS; PR01466; ARGDEIMINASE.
DR   TIGRFAMs; TIGR01078; arcA; 1.
PE   3: Inferred from homology;
KW   Arginine metabolism; Cytoplasm; Hydrolase.
FT   CHAIN           1..409
FT                   /note="Arginine deiminase"
FT                   /id="PRO_0000182246"
FT   ACT_SITE        399
FT                   /note="Amidino-cysteine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00242"
SQ   SEQUENCE   409 AA;  46667 MW;  956B552012BFBC8E CRC64;
     MSSHPIQVFS EIGKLKKVML HRPGKELENL LPDYLERLLF DDIPFLEDAQ KEHDAFAQAL
     RDEGIEVLYL EQLAAESLTS PEIRDQFIEE YLDEANIRDR QTKVAIRELL HGIKDNQELV
     EKTMAGIQKV ELPEIPDEAK DLTDLVESEY PFAIDPMPNL YFTRDPFATI GNAVSLNHMF
     ADTRNRETLY GKYIFKYHPI YGGKVDLVYN REEDTRIEGG DELVLSKDVL AVGISQRTDA
     ASIEKLLVNI FKKNVGFKKV LAFEFANNRK FMHLDTVFTM VDYDKFTIHP EIEGDLHVYS
     VTYENEKLKI VEEKGDLAEL LAQNLGVEKV HLIRCGGGNI VAAAREQWND GSNTLTIAPG
     VVVVYDRNTV TNKILEEYGL RLIKIRGSEL VRGRGGPRCM SMPFEREEV