ID   KV51_NEMVE              Reviewed;          84 AA.
AC   A7RMN1;
DT   19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT   19-MAR-2014, sequence version 2.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Toxin NvePTx1 {ECO:0000303|PubMed:23895459};
DE   Flags: Precursor;
GN   ORFNames=v1g239401;
OS   Nematostella vectensis (Starlet sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Edwardsiidae; Nematostella.
OX   NCBI_TaxID=45351;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CH2 X CH6;
RX   PubMed=17615350; DOI=10.1126/science.1139158;
RA   Putnam N.H., Srivastava M., Hellsten U., Dirks B., Chapman J., Salamov A.,
RA   Terry A., Shapiro H., Lindquist E., Kapitonov V.V., Jurka J.,
RA   Genikhovich G., Grigoriev I.V., Lucas S.M., Steele R.E., Finnerty J.R.,
RA   Technau U., Martindale M.Q., Rokhsar D.S.;
RT   "Sea anemone genome reveals ancestral eumetazoan gene repertoire and
RT   genomic organization.";
RL   Science 317:86-94(2007).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=23895459; DOI=10.1111/febs.12456;
RA   Orts D.J., Moran Y., Cologna C.T., Peigneur S., Madio B., Praher D.,
RA   Quinton L., De Pauw E., Bicudo J.E., Tytgat J., de Freitas J.C.;
RT   "BcsTx3 is a founder of a novel sea anemone toxin family of potassium
RT   channel blocker.";
RL   FEBS J. 280:4839-4852(2013).
RN   [3]
RP   FUNCTION, RECOMBINANT EXPRESSION, DEVELOPMENTAL STAGE, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=29424690; DOI=10.7554/elife.35014;
RA   Columbus-Shenkar Y.Y., Sachkova M.Y., Macrander J., Fridrich A.,
RA   Modepalli V., Reitzel A.M., Sunagar K., Moran Y.;
RT   "Dynamics of venom composition across a complex life cycle.";
RL   Elife 7:0-0(2018).
CC   -!- FUNCTION: Neurotoxin that is probably only defensive (Probable). Acts
CC       as a voltage-gated potassium channel (Kv) inhibitor (By similarity). In
CC       vivo, induces a rapid increase in swimming speed on zebrafish larvae,
CC       as well as death which occurs between 2 and 18 hours later
CC       (PubMed:29424690). {ECO:0000250|UniProtKB:C0HJC4,
CC       ECO:0000269|PubMed:29424690, ECO:0000305|PubMed:29424690}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:C0HJC4}.
CC       Nematocyst {ECO:0000250|UniProtKB:C0HJC4}.
CC   -!- TISSUE SPECIFICITY: In unfertilized eggs and early post-fertilization
CC       stages, is expressed uniformly. In gastrulae, the expression becomes
CC       spatially-localized and seems to be absent from the oral and aboral
CC       poles. In planulae, the expression is clearly observed in the ectoderm
CC       in packed gland cells absent from the two body poles, and upon
CC       metamorphosis, the expression diminishes. There is two types of gland
CC       cells, one large and elongated and another small and round. This toxin
CC       is maternally deposited at both protein and RNA levels.
CC       {ECO:0000269|PubMed:29424690}.
CC   -!- DEVELOPMENTAL STAGE: Highly expressed in unfertilized eggs, late
CC       planulae, primary polyps, and adult females (at protein level). Not
CC       expressed in adult males (at protein level). Consistant with protein
CC       expression, transcripts are highly expressed in unfertilized eggs,
CC       blastulae, gastrulae, early and late planulae, metamorphosing planulae,
CC       and adult females. Its expression is moderate in primary polyps. Its
CC       expression is very low in juvenile polyps, and adult males.
CC       {ECO:0000269|PubMed:29424690}.
CC   -!- PTM: Contains 4 disulfide bonds. {ECO:0000250|UniProtKB:C0HJC4}.
CC   -!- SIMILARITY: Belongs to the sea anemone type 5 potassium channel toxin
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EDO47375.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DS469520; EDO47375.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; XP_001639438.1; XM_001639388.1.
DR   AlphaFoldDB; A7RMN1; -.
DR   SMR; A7RMN1; -.
DR   TCDB; 8.B.19.1.2; the sea anemone k+ channel blocker toxin, bcstx3 (bcstx3) family.
DR   HOGENOM; CLU_1429600_0_0_1; -.
DR   Proteomes; UP000001593; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Disulfide bond;
KW   Ion channel impairing toxin; Nematocyst; Neurotoxin;
KW   Potassium channel impairing toxin; Reference proteome; Secreted; Signal;
KW   Toxin; Voltage-gated potassium channel impairing toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..34
FT                   /evidence="ECO:0000305|PubMed:23895459"
FT                   /id="PRO_0000453816"
FT   CHAIN           35..84
FT                   /note="Toxin NvePTx1"
FT                   /evidence="ECO:0000250|UniProtKB:C0HJC4,
FT                   ECO:0000305|PubMed:23895459"
FT                   /id="PRO_0000425829"
SQ   SEQUENCE   84 AA;  9713 MW;  C17569E21BBC901F CRC64;
     MFSARLVLVF AVVLCIQLCN ASWLDERAMT QEKRCNGKYQ KCTSNSQCCD QKDYAKRKLR
     CLTQCDEGGC MKYKQCMFYA GTQK