KZ_DROME
ID KZ_DROME Reviewed; 1192 AA.
AC O46072;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Probable ATP-dependent RNA helicase kurz;
DE EC=3.6.4.13;
GN Name=kz; ORFNames=CG3228;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP PRELIMINARY NUCLEOTIDE SEQUENCE.
RC STRAIN=Oregon-R;
RX PubMed=1579473; DOI=10.1093/nar/20.7.1802;
RA Tirronen M., Roos C.;
RT "The kurz locus of Drosophila melanogaster shares conserved motifs with a
RT family of yeast ATP dependent splicing factors.";
RL Nucleic Acids Res. 20:1802-1802(1992).
RN [2]
RP SEQUENCE REVISION.
RA Roos C.;
RL Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [6]
RP DEVELOPMENTAL STAGE.
RX PubMed=16453754; DOI=10.1002/j.1460-2075.1987.tb04822.x;
RA Haenlin M., Roos C., Cassab A., Mohier E.;
RT "Oocyte-specific transcription of fs(1)K10: a Drosophila gene affecting
RT dorsal-ventral developmental polarity.";
RL EMBO J. 6:801-807(1987).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-529 AND THR-530, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DEVELOPMENTAL STAGE: Maternal protein detectable up to 6 hours of
CC embryonic development. {ECO:0000269|PubMed:16453754}.
CC -!- MISCELLANEOUS: 'Kurz' means 'short' in German.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DEAH subfamily.
CC {ECO:0000305}.
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DR EMBL; X64418; CAB55570.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF45757.1; -; Genomic_DNA.
DR EMBL; AL009195; CAA15706.1; -; Genomic_DNA.
DR PIR; T13424; T13424.
DR RefSeq; NP_001284815.1; NM_001297886.1.
DR RefSeq; NP_476591.1; NM_057243.4.
DR AlphaFoldDB; O46072; -.
DR SMR; O46072; -.
DR BioGRID; 57743; 2.
DR IntAct; O46072; 5.
DR STRING; 7227.FBpp0070403; -.
DR iPTMnet; O46072; -.
DR PaxDb; O46072; -.
DR EnsemblMetazoa; FBtr0070419; FBpp0070403; FBgn0001330.
DR EnsemblMetazoa; FBtr0342769; FBpp0309621; FBgn0001330.
DR GeneID; 31205; -.
DR KEGG; dme:Dmel_CG3228; -.
DR UCSC; CG3228-RA; d. melanogaster.
DR CTD; 31205; -.
DR FlyBase; FBgn0001330; kz.
DR VEuPathDB; VectorBase:FBgn0001330; -.
DR eggNOG; KOG0926; Eukaryota.
DR HOGENOM; CLU_001832_0_0_1; -.
DR InParanoid; O46072; -.
DR OMA; QICAAGF; -.
DR OrthoDB; 354219at2759; -.
DR PhylomeDB; O46072; -.
DR Reactome; R-DME-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR SignaLink; O46072; -.
DR BioGRID-ORCS; 31205; 0 hits in 1 CRISPR screen.
DR ChiTaRS; sls; fly.
DR GenomeRNAi; 31205; -.
DR PRO; PR:O46072; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0001330; Expressed in egg chamber and 21 other tissues.
DR ExpressionAtlas; O46072; baseline and differential.
DR Genevisible; O46072; DM.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0004386; F:helicase activity; ISS:FlyBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; ISS:FlyBase.
DR GO; GO:0051276; P:chromosome organization; IEA:UniProt.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0022414; P:reproductive process; IEA:UniProt.
DR Gene3D; 3.40.50.300; -; 3.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF04408; HA2; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Phosphoprotein;
KW Reference proteome; RNA-binding.
FT CHAIN 1..1192
FT /note="Probable ATP-dependent RNA helicase kurz"
FT /id="PRO_0000055006"
FT DOMAIN 270..436
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 540..746
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 170..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 504..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 567..612
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 379..382
FT /note="DEAH box"
FT COMPBIAS 184..201
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 579..605
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 283..290
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT MOD_RES 529
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 530
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 1192 AA; 134121 MW; 569D4477D2EF8095 CRC64;
MGKKVHNAKA RQNPQTIVDN SAVKKIQIDV DAVAGGNQVG YDEANALVLP SEKRATKIKV
DKVQHVKILS KKQRKHLQAI VDKKKKKEGR AQLLGDLAAV QIPEEELQQY TSISQVQTVG
LKRLPTLDEY LAKKKERQAQ VLAEKSSASG LRVNAIKGSK RKLLVEEEEE LQAKRKNPNV
ISVEEDDEDS SSSDEDDEEA PAQSAPIAIP TPVSIAPPQI AVKPPIKKLK PEPNPPACIH
QTVYVPVHRT TEVQNARLRL PILAEEQQVM ETINENPIVI VAGETGSGKT TQLPQFLYEA
GYAQHKMIGV TEPRRVAAIA MSKRVAHEMN LPESEVSYLI RFEGNVTPAT RIKFMTDGVL
LKEIETDFLL SKYSVIILDE AHERSVYTDI LVGLLSRIVP LRHKRGQPLK LIIMSATLRV
SDFTENTRLF KIPPPLLKVE ARQFPVTIHF QKRTPDDYVA EAYRKTLKIH NKLPEGGILI
FVTGQQEVNQ LVRKLRRTFP YHHAPTKDVA KNGKVSEEEK EETIDDAAST VEDPKELEFD
MKRVIRNIRK SKKKFLAQMA LPKINLDDYK LPGDDTEADM HEQPDEDDEQ EGLEEDNDDE
LGLEDESGMG SGQRQPLWVL PLYSLLSSEK QNRIFLPVPD GCRLCVVSTN VAETSLTIPH
IKYVVDCGRQ KTRLYDKLTG VSAFVVTYTS KASADQRAGR AGRISAGHCY RLYSSAVYND
CFEDFSQPDI QKKPVEDLML QMRCMGIDRV VHFPFPSPPD QVQLQAAERR LIVLGALEVA
KTENTDLPPA VTRLGHVISR FPVAPRFGKM LALSHQQNLL PYTVCLVAAL SVQEVLIETG
VQRDEDVAPG ANRFHRKRQS WAASGNYQLL GDPMVLLRAV GAAEYAGSQG RLPEFCAANG
LRQKAMSEVR KLRVQLTNEI NLNVSDVELG VDPELKPPTD AQARFLRQIL LAGMGDRVAR
KVPLADIADK EERRRLKYAY NCADMEEPAF LHVSSVLRQK APEWVIYQEA YELQNGDSTK
MFIRGITAIE PEWLLLYVPL LCNIREVRED PAPRFDKTSG KIFCHVDATF GKSGWELPLG
EVEMPLSEKA CCYFGMFLLD GEVCSRLADF RSKLKSTPAS VIKSWSSMNN KVLRFKRALI
TKQIHNRQAL IDQWNSDPHF LLEEYQNLLY DVALSELTPL WPPVDKKEPQ RQ
