L2GL_DROPS
ID L2GL_DROPS Reviewed; 1166 AA.
AC Q08470; Q29NQ3;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 3.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Lethal(2) giant larvae protein;
DE AltName: Full=p127;
GN Name=l(2)gl; ORFNames=GA15420;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=8389031;
RA Toeroek I., Hartenstein K., Kalmes A., Schmitt R., Strand D., Mechler B.M.;
RT "The l(2)gl homologue of Drosophila pseudoobscura suppresses tumorigenicity
RT in transgenic Drosophila melanogaster.";
RL Oncogene 8:1537-1549(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- FUNCTION: Essential for the development of polarized epithelia, for
CC cell polarity associated with asymmetric cell division of neuroblasts
CC during development, and for oocyte polarity formation. Promotes the
CC formation of actin-rich projections at the oocyte cortex and the
CC posterior enrichment of par-1 which is required for oocyte
CC polarization. Regulates the localization of axis-specifying morphogens
CC such as stau and grk (By similarity). Could act as a tumor suppressor.
CC {ECO:0000250, ECO:0000269|PubMed:8389031}.
CC -!- SUBUNIT: May form multimeric complexes. Interacts with mahj. Interacts
CC with aPKC; the interaction results in phosphorylation of l(2)gl.
CC Interacts with ball. {ECO:0000250|UniProtKB:P08111}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:8389031}.
CC Secreted, extracellular space, extracellular matrix
CC {ECO:0000269|PubMed:8389031}. Note=Intercellular matrix.
CC -!- TISSUE SPECIFICITY: Expressed in the epithelial cells of the digestive
CC tract and in gonads, in the ovary's nurse and oocyte's follicle cells.
CC {ECO:0000269|PubMed:8389031}.
CC -!- DEVELOPMENTAL STAGE: Expressed abundantly in early embryogenesis.
CC Moderate expression is found in larval and adult stages.
CC {ECO:0000269|PubMed:8389031}.
CC -!- PTM: Phosphorylated by aPKC which restricts l(2)gl activity to the
CC oocyte posterior and is required for oocyte polarity formation.
CC {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Malignant transformation of the optic centers of
CC the larval brain and the imaginal disks. {ECO:0000269|PubMed:8389031}.
CC -!- SIMILARITY: Belongs to the WD repeat L(2)GL family. {ECO:0000305}.
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DR EMBL; X73259; CAA51715.1; -; Genomic_DNA.
DR EMBL; CH379059; EAL34165.2; -; Genomic_DNA.
DR PIR; S37692; S37692.
DR RefSeq; XP_001357099.2; XM_001357063.3.
DR RefSeq; XP_015035631.1; XM_015180145.1.
DR AlphaFoldDB; Q08470; -.
DR SMR; Q08470; -.
DR STRING; 7237.FBpp0285209; -.
DR EnsemblMetazoa; FBtr0286771; FBpp0285209; FBgn0012725.
DR EnsemblMetazoa; FBtr0366375; FBpp0329550; FBgn0012725.
DR GeneID; 4817731; -.
DR KEGG; dpo:Dpse_GA15420; -.
DR eggNOG; KOG1983; Eukaryota.
DR HOGENOM; CLU_005214_0_0_1; -.
DR InParanoid; Q08470; -.
DR OMA; HACTILI; -.
DR ChiTaRS; l(2)gl; fly.
DR Proteomes; UP000001819; Chromosome 4.
DR Bgee; FBgn0012725; Expressed in female reproductive system and 2 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR000664; Lethal2_giant.
DR InterPro; IPR013577; LLGL2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF08366; LLGL; 1.
DR PRINTS; PR00962; LETHAL2GIANT.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell membrane; Exocytosis; Extracellular matrix; Membrane;
KW Reference proteome; Repeat; Secreted; Tumor suppressor; WD repeat.
FT CHAIN 1..1166
FT /note="Lethal(2) giant larvae protein"
FT /id="PRO_0000084346"
FT REPEAT 39..72
FT /note="WD 1"
FT REPEAT 82..128
FT /note="WD 2"
FT REPEAT 131..167
FT /note="WD 3"
FT REPEAT 189..223
FT /note="WD 4"
FT REPEAT 231..263
FT /note="WD 5"
FT REPEAT 278..320
FT /note="WD 6"
FT REPEAT 328..358
FT /note="WD 7"
FT REPEAT 380..464
FT /note="WD 8"
FT REPEAT 513..595
FT /note="WD 9"
FT REPEAT 604..665
FT /note="WD 10"
FT REPEAT 709..779
FT /note="WD 11"
FT REPEAT 788..833
FT /note="WD 12"
FT REPEAT 838..928
FT /note="WD 13"
FT REPEAT 942..965
FT /note="WD 14"
FT REGION 867..888
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 975..1002
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1144..1166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 47..52
FT /note="AKLMAI -> DQSSWR (in Ref. 1; CAA51715)"
FT /evidence="ECO:0000305"
FT CONFLICT 318
FT /note="H -> Q (in Ref. 1; CAA51715)"
FT /evidence="ECO:0000305"
FT CONFLICT 429..431
FT /note="DEV -> GRKL (in Ref. 1; CAA51715)"
FT /evidence="ECO:0000305"
FT CONFLICT 446
FT /note="S -> T (in Ref. 1; CAA51715)"
FT /evidence="ECO:0000305"
FT CONFLICT 855
FT /note="S -> T (in Ref. 1; CAA51715)"
FT /evidence="ECO:0000305"
FT CONFLICT 969
FT /note="V -> L (in Ref. 1; CAA51715)"
FT /evidence="ECO:0000305"
FT CONFLICT 1003
FT /note="A -> R (in Ref. 1; CAA51715)"
FT /evidence="ECO:0000305"
FT CONFLICT 1150..1152
FT /note="ESE -> DG (in Ref. 1; CAA51715)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1166 AA; 127028 MW; 166A3D56BB03663D CRC64;
MLKFIRGKGQ QPTAERQRLQ KDLFAYRKTA QHGFPHKPSA LAYDPVAKLM AIGTQTGAIK
VFGQPGVELY AQHTLVNNSA AELNVQLLEW VYGTGRILSL TAANQLILWE PVGTTLVPIK
TLPFDGKLKK VSSLCCSLNK DLLWIGTEGG NIYQLDLKSF TIREPVIYHD VVLEQVPPTY
KLNPGAIESI RQLPNSLNKL LIAYNRGLCV LWDMQTSAVE RAYIAPGHGQ SVGLSVNSTG
TEFSWYHADG SYATWSIDNG EPPQNVNYVP YGPDPCKSIN RLYKGQRGTN DVIVFSGGMP
RSAYGDHNCV SVHVSDGHKV CLDFTSKVID FFVTYKEGSD DVEVLIVLLE EELCAYDLTD
PSILSIKAPY LHSVHASAVT CNYLASQVTQ GVYERILRAG DEQDIDYSSI DWPITGGVLT
DDSVESDEDE VVKDYEILLT GHEDGSVKFW DCTGVLLKPI YNFKTASIFG NEHEYREDGA
ADISAEQLDE GEPPFRKAGL FDPYSDDPRL AVKKIAFCPK TGQLVVGGTA GQIVIADFDT
AAEDQSSLKY NSMNLVSDRD GFVWKGHDQL NVRANLLEDN AVPLTENGVN ITGVLQVLPP
ASITCMALEA NWGLVSGGTA HGLVLFDFKN FVPVFHRCTL NPNDLTGAGE QLSRRKSFKK
SLRESFRKLR KGRSTRNNPT NQVPTTLEAR PVERQIEARC TDDGLGSMVR CLLFAKTYVT
NVNITSPTLW SATNASTVSV FLLHLPPAQT AATTVPPASG NVPPQASRRI SAQLAKEIQL
KHRAPVVGIS IFDQTGSPVD QFNAGENGSP PHRLLIASEE QFKVFSLPQL KPINKYKLTA
NEGARIRRIH FGSFSCRISP ELLQSLHSGS PTKSVRSHGE GDGAGNVSAT SAAGRGDLYH
EMALICLTNM GDIMVLSVPE LKRQLNAAAV RREDINGISS LCFTNDGEAL YMMSSSELQR
IALSTSKVVQ PTGIVEVEPL ESEDNETASL VNDGNESDKD GEANKELANT ASDTQPPVAT
LRAKPLELNA DRSSLHLTNG ISNSNSPNRA NETITSSIGD ITVDSVRDHL NTTTTTLCST
TTEETVGRLS VLSTQTNQAT TTVNMKDIPD INIPNLMDLA SKSNTTETST SSVVIKSVIT
SISHEKTNGE SENVTTKTTA HEESQF
