ID   L2HDH_BOVIN             Reviewed;         463 AA.
AC   A7MBI3;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=L-2-hydroxyglutarate dehydrogenase, mitochondrial;
DE            EC=1.1.99.2;
DE   AltName: Full=Duranin;
DE   Flags: Precursor;
GN   Name=L2HGDH;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Ascending colon;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2;
CC         Xref=Rhea:RHEA:21252, ChEBI:CHEBI:13193, ChEBI:CHEBI:16782,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:17499; EC=1.1.99.2;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the L2HGDH family. {ECO:0000305}.
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DR   EMBL; BC151577; AAI51578.1; -; mRNA.
DR   RefSeq; NP_001094560.1; NM_001101090.1.
DR   AlphaFoldDB; A7MBI3; -.
DR   SMR; A7MBI3; -.
DR   STRING; 9913.ENSBTAP00000020226; -.
DR   PaxDb; A7MBI3; -.
DR   PRIDE; A7MBI3; -.
DR   Ensembl; ENSBTAT00000020226; ENSBTAP00000020226; ENSBTAG00000015200.
DR   GeneID; 514230; -.
DR   KEGG; bta:514230; -.
DR   CTD; 79944; -.
DR   VEuPathDB; HostDB:ENSBTAG00000015200; -.
DR   VGNC; VGNC:30761; L2HGDH.
DR   eggNOG; KOG2665; Eukaryota.
DR   GeneTree; ENSGT00490000043421; -.
DR   HOGENOM; CLU_024775_0_0_1; -.
DR   InParanoid; A7MBI3; -.
DR   OMA; GVHFTRM; -.
DR   OrthoDB; 1310154at2759; -.
DR   TreeFam; TF105922; -.
DR   Reactome; R-BTA-880009; Interconversion of 2-oxoglutarate and 2-hydroxyglutarate.
DR   Proteomes; UP000009136; Chromosome 10.
DR   Bgee; ENSBTAG00000015200; Expressed in rectus femoris and 104 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:Ensembl.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0003973; F:(S)-2-hydroxy-acid oxidase activity; IBA:GO_Central.
DR   GO; GO:0047545; F:2-hydroxyglutarate dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0044281; P:small molecule metabolic process; IEA:Ensembl.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01266; DAO; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; FAD; Flavoprotein; Mitochondrion; Oxidoreductase;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..52
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           53..463
FT                   /note="L-2-hydroxyglutarate dehydrogenase, mitochondrial"
FT                   /id="PRO_0000331213"
FT   MOD_RES         104
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q91YP0"
FT   MOD_RES         173
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q91YP0"
SQ   SEQUENCE   463 AA;  51022 MW;  682632966A525F92 CRC64;
     MVPALRYLGS VCGRARGIFP GGFSAAHTPA SGKSRLLCQG GRRASTSSFD IVIIGGGIVG
     LASARALILR HPALSIGVLE KEKNLAVHQT GHNSGVIHSG IYYKPESLKA KLCVQGAALI
     YEYCNQKGIS YKQCGKLIVA VEQEEIPRLQ ALYERGLQNG VQGLRLIQQE DIKKKEPYCR
     GLMAIDCPYT GIVDYRQVAF SFAKDFQEAG GSVLTNFEVE DIEMARESPS RSKDGMKYPI
     VIRNTKGEEV RCQYVVTCAG LYSDRISELS GCNPNPRIVP FRGDYLVLKP EKRYLVKGNI
     YPVPDSRFPF LGVHFTPRMD GNIWLGPNAI LAFKREGYRP FDFSARDIMD IIIKSGLIKL
     VFQNFSYGVN EMYKACFLSA TVKHLQKFIP EITISDVLRG PAGVRAQALD RDGNLIEDFV
     FDGGVGDIGN RILHVRNAPS PAATSSLAIS GMIADEVQQR FKL