ID   L2HDH_DICDI             Reviewed;         446 AA.
AC   Q55GI5;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=L-2-hydroxyglutarate dehydrogenase, mitochondrial;
DE            EC=1.1.99.2;
DE   Flags: Precursor;
GN   Name=l2hgdh; ORFNames=DDB_G0267656;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2;
CC         Xref=Rhea:RHEA:21252, ChEBI:CHEBI:13193, ChEBI:CHEBI:16782,
CC         ChEBI:CHEBI:16810, ChEBI:CHEBI:17499; EC=1.1.99.2;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the L2HGDH family. {ECO:0000305}.
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DR   EMBL; AAFI02000003; EAL73281.1; -; Genomic_DNA.
DR   RefSeq; XP_647199.1; XM_642107.1.
DR   AlphaFoldDB; Q55GI5; -.
DR   SMR; Q55GI5; -.
DR   STRING; 44689.DDB0305262; -.
DR   PaxDb; Q55GI5; -.
DR   EnsemblProtists; EAL73281; EAL73281; DDB_G0267656.
DR   GeneID; 8616003; -.
DR   KEGG; ddi:DDB_G0267656; -.
DR   dictyBase; DDB_G0267656; l2hgdh.
DR   eggNOG; KOG2665; Eukaryota.
DR   HOGENOM; CLU_024775_0_0_1; -.
DR   InParanoid; Q55GI5; -.
DR   OMA; NCAFSKG; -.
DR   PhylomeDB; Q55GI5; -.
DR   Reactome; R-DDI-880009; Interconversion of 2-oxoglutarate and 2-hydroxyglutarate.
DR   PRO; PR:Q55GI5; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0003973; F:(S)-2-hydroxy-acid oxidase activity; IBA:GO_Central.
DR   GO; GO:0047545; F:2-hydroxyglutarate dehydrogenase activity; IBA:GO_Central.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR006076; FAD-dep_OxRdtase.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   Pfam; PF01266; DAO; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   3: Inferred from homology;
KW   FAD; Flavoprotein; Mitochondrion; Oxidoreductase; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..28
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..446
FT                   /note="L-2-hydroxyglutarate dehydrogenase, mitochondrial"
FT                   /id="PRO_0000331212"
SQ   SEQUENCE   446 AA;  49908 MW;  1A4793CFF96350A3 CRC64;
     MKNSSSMLKG VKSFIGSGIY TNKPIYDVAI VGGGIVGLAT GRELLKRNPK LKIVILEKEN
     EIAPHQSSHN SGVIHCGIYY KPGSLRAKLC TKGSKLMYDY CNENQINYEN CGKLIVATKK
     EEFQQLEQLY KRGIENGVPN IKLLESKEQL LSIEPFINGG LRAIHTPSTG IIDYKEVSKS
     FGNDITEKFG KDSKSEIKLN FNAKNFKYNS NDKLLLISTG DDDDDEEQQQ SILTKYSIVC
     GGMNSDRIAK VAYGNDEPSI VPFRGSFLQF KPEFRHLIKG NVYPLPNASF PFLGVHFTKR
     INGEVWLGPN AVLSFDREGY KFTDFNLHDT IDLIKNPGLF KLAKKHWKYG LGELYRDFNK
     DHFIQLLKPY MPNITVDMLE YGGSGVRSQA ISKSGDLIED FIFDTPSDVP IIHVRNSPSP
     AATSSLAIAI EIVDLAQNNF KNLNSL