ID   L5_VACCW                Reviewed;         128 AA.
AC   P68623; P07615; Q76ZT4;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Protein L5;
DE   AltName: Full=Protein F6;
GN   OrderedLocusNames=VACWR092; ORFNames=L5R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2987815; DOI=10.1093/nar/13.3.985;
RA   Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E.;
RT   "Nucleotide sequence of a cluster of early and late genes in a conserved
RT   segment of the vaccinia virus genome.";
RL   Nucleic Acids Res. 13:985-998(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   IDENTIFICATION IN A COMPLEX WITH A16; A21; A28; G3; G9; H2 AND J5.
RX   PubMed=16339313; DOI=10.1073/pnas.0509239102;
RA   Senkevich T.G., Ojeda S., Townsley A., Nelson G.E., Moss B.;
RT   "Poxvirus multiprotein entry-fusion complex.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:18572-18577(2005).
RN   [4]
RP   FUNCTION, AND DISULFIDE BOND.
RX   PubMed=16103150; DOI=10.1128/jvi.79.17.10988-10998.2005;
RA   Townsley A.C., Senkevich T.G., Moss B.;
RT   "The product of the vaccinia virus L5R gene is a fourth membrane protein
RT   encoded by all poxviruses that is required for cell entry and cell-cell
RT   fusion.";
RL   J. Virol. 79:10988-10998(2005).
RN   [5]
RP   INTERACTION WITH G3.
RX   PubMed=21295816; DOI=10.1016/j.virol.2011.01.014;
RA   Wolfe C.L., Moss B.;
RT   "Interaction between the G3 and L5 proteins of the vaccinia virus entry-
RT   fusion complex.";
RL   Virology 412:278-283(2011).
CC   -!- FUNCTION: Envelope protein part of the entry-fusion complex responsible
CC       for the virus membrane fusion with host cell membrane during virus
CC       entry. Also plays a role in cell-cell fusion (syncytium formation)
CC       (Probable). {ECO:0000305|PubMed:16103150}.
CC   -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at least
CC       composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation
CC       of the viral membrane is necessary for the assembly of the complex.
CC       Interacts with G3. {ECO:0000269|PubMed:16339313,
CC       ECO:0000269|PubMed:21295816}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass type
CC       III membrane protein {ECO:0000305}. Note=Component of the mature virion
CC       (MV) membrane (Probable). The mature virion is located in the cytoplasm
CC       of infected cells and is probably released by cell lysis.
CC       {ECO:0000305}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- PTM: Most cysteines are linked by disulfide bonds. They are created by
CC       the viral disulfide bond formation pathway, a poxvirus-specific redox
CC       pathway that operates on the cytoplasmic side of the MV membranes
CC       (Probable). {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae L5 family. {ECO:0000305}.
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DR   EMBL; X01978; CAA26014.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89371.1; -; Genomic_DNA.
DR   PIR; E23092; QQVZF6.
DR   RefSeq; YP_232974.1; NC_006998.1.
DR   SMR; P68623; -.
DR   TCDB; 1.G.11.1.1; the poxvirus cell entry protein complex (pep-c) family.
DR   DNASU; 3707548; -.
DR   GeneID; 3707548; -.
DR   KEGG; vg:3707548; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR006956; Poxvirus_L5.
DR   Pfam; PF04872; Pox_L5; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Fusion of virus membrane with host cell membrane;
KW   Fusion of virus membrane with host membrane; Late protein; Membrane;
KW   Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix;
KW   Viral envelope protein; Viral penetration into host cytoplasm; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..128
FT                   /note="Protein L5"
FT                   /id="PRO_0000099627"
FT   TOPO_DOM        1..30
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        31..51
FT                   /note="Helical; Signal-anchor for type III membrane
FT                   protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        52..128
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   DISULFID        75..107
FT                   /evidence="ECO:0000269|PubMed:16103150"
SQ   SEQUENCE   128 AA;  15044 MW;  3F2D9A60A834ECE5 CRC64;
     MENVPNVYFN PVFIEPTFKH SLLSVYKHRL IVLFEVFVVF ILIYVFFRSE LNMFFMPKRK
     IPDPIDRLRR ANLACEDDKL MIYGLPWMTT QTSALSINSK PIVYKDCAKL LRSINGSQPV
     SLNDVLRR