ID   L5_VAR67                Reviewed;         128 AA.
AC   P0DOU1; P33043;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=Protein L5;
GN   ORFNames=L5R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8383392; DOI=10.1016/0168-1702(93)90110-9;
RA   Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S.,
RA   Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V.,
RA   Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G.,
RA   Sandakhchiev L.S.;
RT   "Nucleotide sequence analysis of variola virus HindIII M, L, I genome
RT   fragments.";
RL   Virus Res. 27:25-35(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Envelope protein part of the entry-fusion complex responsible
CC       for the virus membrane fusion with host cell membrane during virus
CC       entry. Also plays a role in cell-cell fusion (syncytium formation) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of a stable entry-fusion complex (EFC) which is at least
CC       composed of proteins A16, A21, A28, G3, G9, H2, J5, and L5. Formation
CC       of the viral membrane is necessary for the assembly of the complex.
CC       Interacts with G3 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass type
CC       III membrane protein {ECO:0000305}. Note=Component of the mature virion
CC       (MV) membrane (By similarity). The mature virion is located in the
CC       cytoplasm of infected cells and is probably released by cell lysis.
CC       {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- PTM: Most cysteines are linked by disulfide bonds. They are created by
CC       the viral disulfide bond formation pathway, a poxvirus-specific redox
CC       pathway that operates on the cytoplasmic side of the MV membranes (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae L5 family. {ECO:0000305}.
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DR   EMBL; X67119; CAA47576.1; -; Genomic_DNA.
DR   EMBL; S55844; AAB24673.1; -; Genomic_DNA.
DR   EMBL; X69198; CAA49018.1; -; Genomic_DNA.
DR   PIR; S33091; S33091.
DR   RefSeq; NP_042121.1; NC_001611.1.
DR   GeneID; 1486474; -.
DR   KEGG; vg:1486474; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0019064; P:fusion of virus membrane with host plasma membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR006956; Poxvirus_L5.
DR   Pfam; PF04872; Pox_L5; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Fusion of virus membrane with host cell membrane;
KW   Fusion of virus membrane with host membrane; Late protein; Membrane;
KW   Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix;
KW   Viral envelope protein; Viral penetration into host cytoplasm; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..128
FT                   /note="Protein L5"
FT                   /id="PRO_0000099628"
FT   TOPO_DOM        1..30
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        31..51
FT                   /note="Helical; Signal-anchor for type III membrane
FT                   protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        52..107
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   DISULFID        75..107
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   128 AA;  15066 MW;  FC109052D034ECF3 CRC64;
     MENVPNVYFN PVFIEPTFKH SLLSVYKHRL IVLFEVFVVF ILIYVFFRSE LNMFFMHKRK
     IPDPIDRLRR ANLACEDDKL MIYGLPWITT QTSALSINSK PIVYKDCAKL LRSINGSQPV
     SLNDVLRR