LA1_ARATH
ID LA1_ARATH Reviewed; 433 AA.
AC Q93ZV7; F4JV47; O65529; Q8L7E4;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=La protein 1;
DE Short=AtLa1;
GN Name=LA1; OrderedLocusNames=At4g32720; ORFNames=F4D11.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND IDENTIFICATION.
RC STRAIN=cv. Columbia;
RX PubMed=17459889; DOI=10.1093/nar/gkm200;
RA Fleurdepine S., Deragon J.M., Devic M., Guilleminot J.,
RA Bousquet-Antonelli C.;
RT "A bona fide La protein is required for embryogenesis in Arabidopsis
RT thaliana.";
RL Nucleic Acids Res. 35:3306-3321(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19299548; DOI=10.1261/rna.1478709;
RA Bousquet-Antonelli C., Deragon J.M.;
RT "A comprehensive analysis of the La-motif protein superfamily.";
RL RNA 15:750-764(2009).
CC -!- FUNCTION: Binds to the 3' poly(U) terminus of nascent RNA polymerase
CC III transcripts, protecting them from exonuclease digestion and
CC facilitating their folding and maturation.
CC {ECO:0000269|PubMed:17459889}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:17459889}. Nucleus, nucleolus
CC {ECO:0000269|PubMed:17459889}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q93ZV7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q93ZV7-2; Sequence=VSP_054170;
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously (at protein level).
CC {ECO:0000269|PubMed:17459889}.
CC -!- DISRUPTION PHENOTYPE: Embryonic-lethal with deficient embryos arrested
CC at early globular stage of development characterized by nucleolar
CC hypertrophy. {ECO:0000269|PubMed:17459889}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA18589.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79989.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL022537; CAA18589.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161582; CAB79989.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86109.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86110.1; -; Genomic_DNA.
DR EMBL; AY056237; AAL07086.1; -; mRNA.
DR EMBL; AY136302; AAM96968.1; -; mRNA.
DR EMBL; BT000396; AAN15715.1; -; mRNA.
DR PIR; T04453; T04453.
DR RefSeq; NP_001031777.1; NM_001036700.2. [Q93ZV7-2]
DR RefSeq; NP_567904.1; NM_119425.5. [Q93ZV7-1]
DR AlphaFoldDB; Q93ZV7; -.
DR SMR; Q93ZV7; -.
DR BioGRID; 14694; 7.
DR STRING; 3702.AT4G32720.1; -.
DR iPTMnet; Q93ZV7; -.
DR PaxDb; Q93ZV7; -.
DR PRIDE; Q93ZV7; -.
DR ProteomicsDB; 237144; -. [Q93ZV7-1]
DR EnsemblPlants; AT4G32720.1; AT4G32720.1; AT4G32720. [Q93ZV7-1]
DR EnsemblPlants; AT4G32720.2; AT4G32720.2; AT4G32720. [Q93ZV7-2]
DR GeneID; 829408; -.
DR Gramene; AT4G32720.1; AT4G32720.1; AT4G32720. [Q93ZV7-1]
DR Gramene; AT4G32720.2; AT4G32720.2; AT4G32720. [Q93ZV7-2]
DR KEGG; ath:AT4G32720; -.
DR Araport; AT4G32720; -.
DR TAIR; locus:2125702; AT4G32720.
DR eggNOG; KOG0118; Eukaryota.
DR InParanoid; Q93ZV7; -.
DR OMA; QFERSIY; -.
DR PhylomeDB; Q93ZV7; -.
DR PRO; PR:Q93ZV7; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q93ZV7; baseline and differential.
DR Genevisible; Q93ZV7; AT.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR GO; GO:1990904; C:ribonucleoprotein complex; IEA:InterPro.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IDA:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0034470; P:ncRNA processing; IDA:UniProtKB.
DR GO; GO:0042254; P:ribosome biogenesis; IMP:TAIR.
DR GO; GO:0006364; P:rRNA processing; IMP:TAIR.
DR GO; GO:0008033; P:tRNA processing; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR045180; La_dom_prot.
DR InterPro; IPR006630; La_HTH.
DR InterPro; IPR014886; La_xRRM.
DR InterPro; IPR002344; Lupus_La.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR22792; PTHR22792; 1.
DR Pfam; PF05383; La; 1.
DR Pfam; PF00076; RRM_1; 1.
DR Pfam; PF08777; RRM_3; 1.
DR PRINTS; PR00302; LUPUSLA.
DR SMART; SM00715; LA; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50961; HTH_LA; 1.
DR PROSITE; PS50102; RRM; 1.
DR PROSITE; PS51939; XRRM; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..433
FT /note="La protein 1"
FT /id="PRO_0000428664"
FT DOMAIN 4..108
FT /note="HTH La-type RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00332"
FT DOMAIN 116..193
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 298..419
FT /note="xRRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01288"
FT REGION 206..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 245..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 395..433
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 209..226
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 248..287
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 405..433
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_054170"
FT CONFLICT 231
FT /note="P -> Q (in Ref. 3; AAM96968/AAN15715)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 433 AA; 48096 MW; E58EBAF51C35A8F7 CRC64;
MSIPCLTEET AKTVLRQVEF YFSDSNLPID DFLKKTVTES EDGLVSLALI CSFSKMRGYL
KLGDSKGDDI PEDTIKAVAD TLRTSSALKI SDDGKKVGRS TELLKLEDLI EQLNARTVAA
SPFSYDVKRE DVESFFSQYG KVNSVRMPRH VAESRIFSGV ALVEFPTEED AQNVMKQNLV
FAGQELELKP KKEFDNEREK DEVKFANYQP QKGSANQKNG SDHKNNSAYE PDYPKGLIIS
FTLKRSAEEG TTEQKSSEEP TDKTMEESET KPADTPDADK ENTGEVQAEG AEDEDDEKEE
KGALATHKDN KDVVLREDLK AVFGKFGDVK FVDFKMGSET GYLRFDEPEA SQKARAAAVL
ANEGGLAVKN FIAVLEPVIG EAEKEYWTLL RSKDRFDKGG RGGRGGRRGG RFGRKRGSDS
PGGRWNKSQK VEA
