LA2_ARATH
ID LA2_ARATH Reviewed; 399 AA.
AC Q0V7U7; C0Z3H3; F4HQC8; Q0WRW1; Q9CA91;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=La protein 2;
DE Short=AtLa2;
GN Name=LA2; OrderedLocusNames=At1g79880; ORFNames=F19K16.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF Clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP TISSUE SPECIFICITY, AND IDENTIFICATION.
RC STRAIN=cv. Columbia;
RX PubMed=17459889; DOI=10.1093/nar/gkm200;
RA Fleurdepine S., Deragon J.M., Devic M., Guilleminot J.,
RA Bousquet-Antonelli C.;
RT "A bona fide La protein is required for embryogenesis in Arabidopsis
RT thaliana.";
RL Nucleic Acids Res. 35:3306-3321(2007).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19299548; DOI=10.1261/rna.1478709;
RA Bousquet-Antonelli C., Deragon J.M.;
RT "A comprehensive analysis of the La-motif protein superfamily.";
RL RNA 15:750-764(2009).
CC -!- FUNCTION: Binds to the 3' poly(U) terminus of nascent RNA polymerase
CC III transcripts, protecting them from exonuclease digestion and
CC facilitating their folding and maturation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm {ECO:0000250}. Nucleus,
CC nucleolus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q0V7U7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0V7U7-2; Sequence=VSP_054171;
CC Name=3;
CC IsoId=Q0V7U7-3; Sequence=VSP_054171, VSP_054172;
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously (at protein level).
CC {ECO:0000269|PubMed:17459889}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52256.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC011717; AAG52256.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE36317.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36318.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36319.1; -; Genomic_DNA.
DR EMBL; AK319137; BAH57252.1; -; mRNA.
DR EMBL; AK228183; BAF00138.1; -; mRNA.
DR EMBL; BT026473; ABH04580.1; -; mRNA.
DR PIR; H96829; H96829.
DR RefSeq; NP_001319421.1; NM_001334930.1. [Q0V7U7-2]
DR RefSeq; NP_178106.2; NM_106637.5. [Q0V7U7-1]
DR RefSeq; NP_974185.1; NM_202456.2. [Q0V7U7-3]
DR AlphaFoldDB; Q0V7U7; -.
DR SMR; Q0V7U7; -.
DR STRING; 3702.AT1G79880.1; -.
DR PaxDb; Q0V7U7; -.
DR PRIDE; Q0V7U7; -.
DR ProteomicsDB; 237040; -. [Q0V7U7-1]
DR EnsemblPlants; AT1G79880.1; AT1G79880.1; AT1G79880. [Q0V7U7-1]
DR EnsemblPlants; AT1G79880.2; AT1G79880.2; AT1G79880. [Q0V7U7-2]
DR EnsemblPlants; AT1G79880.3; AT1G79880.3; AT1G79880. [Q0V7U7-3]
DR GeneID; 844327; -.
DR Gramene; AT1G79880.1; AT1G79880.1; AT1G79880. [Q0V7U7-1]
DR Gramene; AT1G79880.2; AT1G79880.2; AT1G79880. [Q0V7U7-2]
DR Gramene; AT1G79880.3; AT1G79880.3; AT1G79880. [Q0V7U7-3]
DR KEGG; ath:AT1G79880; -.
DR Araport; AT1G79880; -.
DR TAIR; locus:2017809; AT1G79880.
DR eggNOG; KOG0118; Eukaryota.
DR HOGENOM; CLU_051929_0_0_1; -.
DR InParanoid; Q0V7U7; -.
DR OMA; WENIDNE; -.
DR OrthoDB; 882725at2759; -.
DR PhylomeDB; Q0V7U7; -.
DR PRO; PR:Q0V7U7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q0V7U7; baseline and differential.
DR Genevisible; Q0V7U7; AT.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:1990904; C:ribonucleoprotein complex; IEA:InterPro.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0034470; P:ncRNA processing; ISS:UniProtKB.
DR GO; GO:0008033; P:tRNA processing; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR045180; La_dom_prot.
DR InterPro; IPR006630; La_HTH.
DR InterPro; IPR014886; La_xRRM.
DR InterPro; IPR002344; Lupus_La.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR22792; PTHR22792; 2.
DR Pfam; PF05383; La; 1.
DR Pfam; PF00076; RRM_1; 1.
DR Pfam; PF08777; RRM_3; 1.
DR PRINTS; PR00302; LUPUSLA.
DR SMART; SM00715; LA; 1.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50961; HTH_LA; 1.
DR PROSITE; PS50102; RRM; 1.
DR PROSITE; PS51939; XRRM; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..399
FT /note="La protein 2"
FT /id="PRO_0000428665"
FT DOMAIN 3..106
FT /note="HTH La-type RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00332"
FT DOMAIN 115..192
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 269..399
FT /note="xRRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01288"
FT REGION 367..399
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..54
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_054171"
FT VAR_SEQ 372
FT /note="G -> GYDIKPNLVYSSL (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_054172"
FT CONFLICT 202
FT /note="K -> R (in Ref. 4; BAF00138)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 399 AA; 44926 MW; 5A901094DF833E08 CRC64;
MASSFNEETA KKLLTQVEFY FSDSNLPTDG FLNREVTKSK DGLVSLPLVC SFSRMRNLLG
LGNINREDIP PRIVEEVANL LRTSDFLKVS NNGQRIGRGT KLSKPEEVLE QVHRRTLAAS
PFEYSIKMED VSSFFSQYAK VNSVRLPPNI ADKRRFCGTA LVEFSSEQDT QDILRQSLVY
AGADLVLIPK SDFDCQRENM IKQLGKSESH NEFRRGQIVK FALKWIASEE KVTNKEKPSA
LKNKIKEKED KETGIADREK ENGDNSCASL CKDNTDQLVV PPWNNSNSVS SEVLKDLFQR
FGSVEHIEYS GGLDSGYVWF TDSETAMKAR AAVEFVGGLV VKNNFSVALE AINGEMEREL
WKRLSSAELE GGKEGHKKEK GKDECFENVQ PTKKARKEP
