LAC2_CERUI
ID LAC2_CERUI Reviewed; 10 AA.
AC P85149;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 29-SEP-2021, entry version 31.
DE RecName: Full=Laccase-2;
DE EC=1.10.3.2 {ECO:0000269|Ref.1};
DE AltName: Full=Benzenediol:oxygen oxidoreductase 2;
DE AltName: Full=Diphenol oxidase 2;
DE AltName: Full=Lac II;
DE AltName: Full=Urishiol oxidase 2;
DE Flags: Fragment;
OS Cerrena unicolor (Canker rot fungus) (Daedalea unicolor).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Polyporales; Cerrenaceae; Cerrena.
OX NCBI_TaxID=90312;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP GLYCOSYLATION.
RA Janusz G., Rogalski J.M.;
RT "Purification of Cerrena unicolor laccase.";
RL Submitted (APR-2007) to UniProtKB.
CC -!- FUNCTION: Lignin degradation and detoxification of lignin-derived
CC products. {ECO:0000250|UniProtKB:Q12718, ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O;
CC Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2;
CC Evidence={ECO:0000269|Ref.1};
CC -!- COFACTOR:
CC Name=Cu cation; Xref=ChEBI:CHEBI:23378;
CC Evidence={ECO:0000250|UniProtKB:Q12718};
CC Note=Binds 4 Cu cations per monomer. {ECO:0000250|UniProtKB:Q12718};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.166 mM for syringaldazine {ECO:0000269|Ref.1};
CC pH dependence:
CC Optimum pH is 5.3. {ECO:0000269|Ref.1};
CC Temperature dependence:
CC Optimum temperature is 60 degrees Celsius. {ECO:0000269|Ref.1};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q12718}.
CC -!- PTM: Glycosylated; contains 8.47% carbohydrates. {ECO:0000269|Ref.1}.
CC -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is:
CC 4.56, its MW is: 62.03 kDa. {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the multicopper oxidase family. {ECO:0000255}.
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DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0052716; F:hydroquinone:oxygen oxidoreductase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0046274; P:lignin catabolic process; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Copper; Direct protein sequencing; Glycoprotein; Lignin degradation;
KW Metal-binding; Oxidoreductase; Secreted.
FT CHAIN 1..>10
FT /note="Laccase-2"
FT /id="PRO_0000288803"
FT NON_TER 10
FT /evidence="ECO:0000303|Ref.1"
SQ SEQUENCE 10 AA; 1005 MW; 212CE22AADC2D768 CRC64;
AIGPVADLHI