位置:首页 > 蛋白库 > LAC9_KLULA
LAC9_KLULA
ID   LAC9_KLULA              Reviewed;         865 AA.
AC   P08657;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   03-AUG-2022, entry version 154.
DE   RecName: Full=Lactose regulatory protein LAC9;
GN   Name=LAC9; OrderedLocusNames=KLLA0D12672g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3550430; DOI=10.1128/mcb.7.3.1111-1121.1987;
RA   Wray L.V. Jr., Witte M.M., Dickson R.C., Riley M.I.;
RT   "Characterization of a positive regulatory gene, LAC9, that controls
RT   induction of the lactose-galactose regulon of Kluyveromyces lactis:
RT   structural and functional relationships to GAL4 of Saccharomyces
RT   cerevisiae.";
RL   Mol. Cell. Biol. 7:1111-1121(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=3022234; DOI=10.1093/nar/14.19.7767;
RA   Salmeron J.M., Johnston S.A.;
RT   "Analysis of the Kluyveromyces lactis positive regulatory gene LAC9 reveals
RT   functional homology to, but sequence divergence from, the Saccharomyces
RT   cerevisiae GAL4 gene.";
RL   Nucleic Acids Res. 14:7767-7781(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
RN   [4]
RP   MUTAGENESIS.
RX   PubMed=2107531; DOI=10.1093/nar/18.4.745;
RA   Kuger P., Goedecke A., Breunig K.D.;
RT   "A mutation in the Zn-finger of the GAL4 homolog LAC9 results in glucose
RT   repression of its target genes.";
RL   Nucleic Acids Res. 18:745-751(1990).
RN   [5]
RP   STRUCTURE BY NMR OF 85-144.
RX   PubMed=7552715; DOI=10.1038/nsb1095-898;
RA   Gardner K.H., Anderson S.F., Coleman J.E.;
RT   "Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding
RT   domain.";
RL   Nat. Struct. Biol. 2:898-905(1995).
CC   -!- FUNCTION: Positive regulatory protein, that controls induction of the
CC       lactose-galactose regulation of Kluyveromyces lactis.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M15210; AAA35266.1; -; Genomic_DNA.
DR   EMBL; X06215; CAA29565.1; -; Genomic_DNA.
DR   EMBL; CR382124; CAH00723.1; -; Genomic_DNA.
DR   PIR; A93641; A25762.
DR   PIR; S08482; S08482.
DR   RefSeq; XP_453627.1; XM_453627.1.
DR   PDB; 1CLD; NMR; -; A=85-144.
DR   PDB; 3E1K; X-ray; 3.00 A; B/D/F/H/J/L/N/P=844-865.
DR   PDBsum; 1CLD; -.
DR   PDBsum; 3E1K; -.
DR   AlphaFoldDB; P08657; -.
DR   SMR; P08657; -.
DR   STRING; 28985.XP_453627.1; -.
DR   EnsemblFungi; CAH00723; CAH00723; KLLA0_D12672g.
DR   GeneID; 2893282; -.
DR   KEGG; kla:KLLA0_D12672g; -.
DR   eggNOG; ENOG502QSMN; Eukaryota.
DR   HOGENOM; CLU_008599_2_0_1; -.
DR   InParanoid; P08657; -.
DR   OMA; SYPFIHE; -.
DR   EvolutionaryTrace; P08657; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006012; P:galactose metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   CDD; cd14654; ZIP_Gal4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR046347; bZIP_sf.
DR   InterPro; IPR005600; Gal4_dimer_dom.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF03902; Gal4_dimer; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00906; Fungal_trans; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   SUPFAM; SSF57959; SSF57959; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; Carbohydrate metabolism; DNA-binding;
KW   Galactose metabolism; Metal-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation; Zinc.
FT   CHAIN           1..865
FT                   /note="Lactose regulatory protein LAC9"
FT                   /id="PRO_0000114953"
FT   DNA_BIND        95..122
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..87
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          818..840
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..19
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        23..50
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        60..87
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         95
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   BINDING         95
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   BINDING         98
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   BINDING         105
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   BINDING         112
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT   BINDING         112
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   BINDING         115
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   BINDING         122
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT   HELIX           96..100
FT                   /evidence="ECO:0007829|PDB:1CLD"
FT   STRAND          109..111
FT                   /evidence="ECO:0007829|PDB:1CLD"
FT   HELIX           113..118
FT                   /evidence="ECO:0007829|PDB:1CLD"
FT   HELIX           853..859
FT                   /evidence="ECO:0007829|PDB:3E1K"
SQ   SEQUENCE   865 AA;  97038 MW;  D6CC912F238DBEBE CRC64;
     MGSRASNSPS FSSKAETLLP SEYKKNAVKK ETIRNGKKRK LPDTESSDPE FASRRLIANE
     TGTDAVSNGN KNDSNANNNN NNNNKKSSEV MHQACDACRK KKWKCSKTVP TCTNCLKYNL
     DCVYSPQVVR TPLTRAHLTE MENRVAELEQ FLKELFPVWD IDRLLQQKDT YRIRELLTMG
     STNTVPGLAS NNIDSSLEQP VAFGTAQPAQ SLSTDPAVQS QAYPMQPVPM TELQSITNLR
     HTPSLLDEQQ MNTISTATLR NMYSSGNNNN NLGNISGLSP VTEAFFRWQE GETSIDNSYF
     GKGSILFWLN QLLSSEKIAG VTSKVGNDIN TNNNNINHQK LPLILNNNIT HNVSDITTTS
     TSSNKRAMSP LSANDSVYLA KRETISAYID AYFKHYHALY PLVSKEMFFA QYNDQIKPEN
     VEIWHILLNA VLALGSWCSN SCSSHHTLYY QNALSYLSTA VLETGSTDLT IALILLTHYV
     QKMHKPNTAW SLIGLCSHMA TSLGLHRDLP NSTIHDQQLR RVLWWTIYCT GCDLSLETGR
     PSLLPNLQAI DIPLPASSAT IKEPSIYSSI IQESQWSQIL QQKLSNNSYQ QSAGECLSWF
     DSVQAFLDHW PTPSTEAELK ALNETQLDWL PLVKFRPYWM FHCSLISLFS VFFEEDAPTD
     NNVIRCKELC LQLSSRNIFS VATFVRSYAF NSLSCWYATH YLVRSALVPL HFASRISPQH
     ALWETVKAQL LSAHEAMGIL SQESSLAAKF DGILTKNYSE ILQREGINKS QLMPPPTPLL
     QSTSFSDLLS LWSANAEDAP RVSNSQMPQS ITITDSLLQS STTQMRPPTT SGWPDTNNFL
     NPSTQQLFNT TTMDDVYNYI FDNDE
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024