ID   LACB_BUBBU              Reviewed;         180 AA.
AC   P02755; O62822;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2002, sequence version 2.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Beta-lactoglobulin;
DE            Short=Beta-LG;
DE   Flags: Precursor;
GN   Name=LGB;
OS   Bubalus bubalis (Domestic water buffalo).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bubalus.
OX   NCBI_TaxID=89462;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Mammary gland;
RX   PubMed=10376212; DOI=10.3109/10425179909008426;
RA   Das P., Jain S., Nayak S., Apparao K.B.C., Totey S.M., Garg L.C.;
RT   "Molecular cloning and sequence analysis of the cDNA encoding beta-
RT   lactoglobulin in Bubalus bubalis.";
RL   DNA Seq. 10:105-108(1999).
RN   [2]
RP   PROTEIN SEQUENCE OF 19-180.
RA   Kolde H.-J., Liberatori J., Braunitzer G.;
RT   "The amino acid sequence of the water buffalo beta-lactoglobulin.";
RL   Milchwissenschaft 36:83-86(1981).
CC   -!- FUNCTION: Primary component of whey, it binds retinol and is probably
CC       involved in the transport of that molecule.
CC   -!- SUBUNIT: Under physiological conditions beta-lactoglobulin exists as an
CC       equilibrium mixture of monomeric and dimeric forms (By similarity).
CC       Interaction with LMBR1L is controversial (By similarity).
CC       {ECO:0000250|UniProtKB:P02754}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Synthesized in mammary gland and secreted in milk.
CC   -!- PTM: Alternate disulfide bonds occur in equal amounts.
CC   -!- SIMILARITY: Belongs to the calycin superfamily. Lipocalin family.
CC       {ECO:0000305}.
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DR   EMBL; AJ005429; CAA06532.1; -; mRNA.
DR   PIR; A03219; LGBUI.
DR   AlphaFoldDB; P02755; -.
DR   BMRB; P02755; -.
DR   SMR; P02755; -.
DR   Allergome; 1258; Bub b 5.
DR   PRIDE; P02755; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019841; F:retinol binding; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.128.20; -; 1.
DR   InterPro; IPR002447; Blactoglobulin.
DR   InterPro; IPR012674; Calycin.
DR   InterPro; IPR002345; Lipocalin.
DR   InterPro; IPR022272; Lipocalin_CS.
DR   InterPro; IPR000566; Lipocln_cytosolic_FA-bd_dom.
DR   PANTHER; PTHR11430; PTHR11430; 1.
DR   Pfam; PF00061; Lipocalin; 1.
DR   PRINTS; PR01172; BLCTOGLOBULN.
DR   SUPFAM; SSF50814; SSF50814; 1.
DR   PROSITE; PS00213; LIPOCALIN; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Milk protein; Retinol-binding;
KW   Secreted; Signal; Transport.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000269|Ref.2"
FT   CHAIN           19..180
FT                   /note="Beta-lactoglobulin"
FT                   /id="PRO_0000017904"
FT   DISULFID        84..178
FT   DISULFID        124..139
FT                   /note="Alternate"
FT   DISULFID        124..137
SQ   SEQUENCE   180 AA;  20023 MW;  6836C97B2C2E33CF CRC64;
     MKCLLLALGL ALACAAQAII VTQTMKGLDI QKVAGTWYSL AMAASDISLL DAQSAPLRVY
     VEELKPTPEG DLEILLQKWE NGECAQKKII AEKTKIPAVF KIDALNENKV LVLDTDYKKY
     LLFCMENSAE PEQSLACQCL VRTPEVDDEA LEKFDKALKA LPMHIRLSFN PTQLEEQCHV