ID   LACD_LACLL              Reviewed;         326 AA.
AC   P26593;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Tagatose 1,6-diphosphate aldolase;
DE            EC=4.1.2.40;
DE   AltName: Full=D-tagatose-1,6-bisphosphate aldolase;
DE   AltName: Full=Tagatose-bisphosphate aldolase;
GN   Name=lacD;
OS   Lactococcus lactis subsp. lactis (Streptococcus lactis).
OG   Plasmid pLP712.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], OPERON STRUCTURE, AND INDUCTION.
RC   STRAIN=MG1820;
RX   PubMed=1901863; DOI=10.1016/s0021-9258(20)89626-4;
RA   van Rooijen R.J., van Schalkwijk S., de Vos W.M.;
RT   "Molecular cloning, characterization, and nucleotide sequence of the
RT   tagatose 6-phosphate pathway gene cluster of the lactose operon of
RT   Lactococcus lactis.";
RL   J. Biol. Chem. 266:7176-7181(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 317-326.
RC   STRAIN=MG1820;
RX   PubMed=2125052; DOI=10.1016/s0021-9258(18)45741-9;
RA   de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W.;
RT   "Characterization of the lactose-specific enzymes of the phosphotransferase
RT   system in Lactococcus lactis.";
RL   J. Biol. Chem. 265:22554-22560(1990).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-tagatofuranose 1,6-bisphosphate = D-glyceraldehyde 3-
CC         phosphate + dihydroxyacetone phosphate; Xref=Rhea:RHEA:22948,
CC         ChEBI:CHEBI:57642, ChEBI:CHEBI:58694, ChEBI:CHEBI:59776; EC=4.1.2.40;
CC   -!- PATHWAY: Carbohydrate metabolism; D-tagatose 6-phosphate degradation;
CC       D-glyceraldehyde 3-phosphate and glycerone phosphate from D-tagatose 6-
CC       phosphate: step 2/2.
CC   -!- INDUCTION: By lactose. The operon consists of lacABCDFEGX.
CC       {ECO:0000269|PubMed:1901863}.
CC   -!- MISCELLANEOUS: This gene was sequenced from pMG820, a laboratory-
CC       derived deletion of the naturally occurring plasmid pLP712.
CC   -!- SIMILARITY: Belongs to the aldolase LacD family. {ECO:0000305}.
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DR   EMBL; M60447; AAA25180.1; -; Genomic_DNA.
DR   EMBL; M65190; AAA25171.1; -; Genomic_DNA.
DR   PIR; D39778; D39778.
DR   AlphaFoldDB; P26593; -.
DR   SMR; P26593; -.
DR   PRIDE; P26593; -.
DR   UniPathway; UPA00704; UER00716.
DR   GO; GO:0009024; F:tagatose-6-phosphate kinase activity; IEA:InterPro.
DR   GO; GO:0009025; F:tagatose-bisphosphate aldolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:2001059; P:D-tagatose 6-phosphate catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0019512; P:lactose catabolic process via tagatose-6-phosphate; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_00734; LacD; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002915; DeoC/FbaB/LacD_aldolase.
DR   InterPro; IPR005927; Tag_1.6-dipho_adolase.
DR   Pfam; PF01791; DeoC; 1.
DR   SMART; SM01133; DeoC; 1.
DR   TIGRFAMs; TIGR01232; lacD; 1.
PE   2: Evidence at transcript level;
KW   Lactose metabolism; Lyase; Plasmid.
FT   CHAIN           1..326
FT                   /note="Tagatose 1,6-diphosphate aldolase"
FT                   /id="PRO_0000203940"
SQ   SEQUENCE   326 AA;  36476 MW;  37F22F556F47941B CRC64;
     MVLTEQKRKS LEKLSDKNGF ISALAFDQRG ALKRLMAQYQ DTEPTVAQME ELKVLVADEL
     TKYASSMLLD PEYGLPATKA LDKEAGLLLA FEKTGYDTSS TKRLPDCLDV WSAKRIKEQG
     ADAVKFLLYY DVDSSDELNQ QKQAYIERVG SECVAEDIPF FLEILAYDEE ISDAGSVEYA
     KVKPRKVIEA MKVFSDPRFN IDVLKVEVPV NVKYVEGFAD GEVVYSKAEA ADFFKAQEEA
     TNLPYIYLSA GVSAKLFQET LQFAHDSGAK FNGVLCGRAT WAGSVEPYIK EGEKAAREWL
     RTTGFENIDE LNKVLVKTAS PWTDKV