ID   LACG1_STRPN             Reviewed;         470 AA.
AC   Q97SA9;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=6-phospho-beta-galactosidase 1 {ECO:0000255|HAMAP-Rule:MF_01574};
DE            EC=3.2.1.85 {ECO:0000255|HAMAP-Rule:MF_01574};
DE   AltName: Full=Beta-D-phosphogalactoside galactohydrolase 1 {ECO:0000255|HAMAP-Rule:MF_01574};
DE            Short=PGALase 1 {ECO:0000255|HAMAP-Rule:MF_01574};
DE   AltName: Full=P-beta-Gal 1 {ECO:0000255|HAMAP-Rule:MF_01574};
DE            Short=PBG 1 {ECO:0000255|HAMAP-Rule:MF_01574};
GN   Name=lacG1 {ECO:0000255|HAMAP-Rule:MF_01574}; Synonyms=lacG-1;
GN   OrderedLocusNames=SP_0477;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 6-phospho-beta-D-galactoside + H2O = an alcohol + D-
CC         galactose 6-phosphate; Xref=Rhea:RHEA:24568, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:58534, ChEBI:CHEBI:91004; EC=3.2.1.85;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01574};
CC   -!- PATHWAY: Carbohydrate metabolism; lactose degradation; D-galactose 6-
CC       phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
CC       {ECO:0000255|HAMAP-Rule:MF_01574}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01574}.
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DR   EMBL; AE005672; AAK74636.1; -; Genomic_DNA.
DR   PIR; C95055; C95055.
DR   RefSeq; WP_000429431.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; Q97SA9; -.
DR   SMR; Q97SA9; -.
DR   STRING; 170187.SP_0477; -.
DR   CAZy; GH1; Glycoside Hydrolase Family 1.
DR   EnsemblBacteria; AAK74636; AAK74636; SP_0477.
DR   KEGG; spn:SP_0477; -.
DR   eggNOG; COG2723; Bacteria.
DR   OMA; VEQWITL; -.
DR   PhylomeDB; Q97SA9; -.
DR   BioCyc; SPNE170187:G1FZB-494-MON; -.
DR   UniPathway; UPA00542; UER00605.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0033920; F:6-phospho-beta-galactosidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019512; P:lactose catabolic process via tagatose-6-phosphate; IEA:InterPro.
DR   HAMAP; MF_01574; LacG; 1.
DR   InterPro; IPR005928; 6P-beta-galactosidase.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; PTHR10353; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 1.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   TIGRFAMs; TIGR01233; lacG; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase.
FT   CHAIN           1..470
FT                   /note="6-phospho-beta-galactosidase 1"
FT                   /id="PRO_0000260730"
FT   ACT_SITE        164
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT   ACT_SITE        378
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
SQ   SEQUENCE   470 AA;  54633 MW;  964D7022454360C6 CRC64;
     MENLQVKALP KEFLLGTATA AYQVEGATRV DGKGINMWDV YLQENSPFLP DPASDFYYRY
     EEDIALAAEH GLQALRLSIS WVRIFPDIDG DANVLAVHYY HRVFQSCLKH NVIPFVSLHH
     FDSPQKMLET GDWLNRENID RFIRYARFCF QEFTEVKHWF TINELMSLAA GQYIGGQFPP
     NHHFQLSEAI QANHNMLLAH ALAVLEFHQL GIEGKVGCIH ALKPGYPIDG QKENILAAKR
     YDVYNNKFLL DGTFLGYYSE DTLFHLNQIL EANNSSFIIE DGDLEIMKRA APLNTMFGMN
     YYRSEFIREY KGENRQEFNS TGIKGQSSFK LNALGEFVKK PGIPTTDWDW NIYPQGLFDM
     LLRIKEEYPQ HPVIYLTENG TALKEVKPEG ENDIIDDSKR IRYIEQHLHK VLEARDRGVN
     IQGYFIWSLQ DQFSWANGYN KRYGLFFVDY ETQKRYIKKS ALWVKGLKRN