ID   LACG1_STRR6             Reviewed;         470 AA.
AC   Q8DQZ1;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=6-phospho-beta-galactosidase 1 {ECO:0000255|HAMAP-Rule:MF_01574};
DE            EC=3.2.1.85 {ECO:0000255|HAMAP-Rule:MF_01574};
DE   AltName: Full=Beta-D-phosphogalactoside galactohydrolase 1 {ECO:0000255|HAMAP-Rule:MF_01574};
DE            Short=PGALase 1 {ECO:0000255|HAMAP-Rule:MF_01574};
DE   AltName: Full=P-beta-Gal 1 {ECO:0000255|HAMAP-Rule:MF_01574};
DE            Short=PBG 1 {ECO:0000255|HAMAP-Rule:MF_01574};
GN   Name=lacG1 {ECO:0000255|HAMAP-Rule:MF_01574}; OrderedLocusNames=spr0424;
OS   Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=171101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-255 / R6;
RX   PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA   Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA   DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA   Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA   Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA   McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA   Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA   Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA   Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT   "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL   J. Bacteriol. 183:5709-5717(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 6-phospho-beta-D-galactoside + H2O = an alcohol + D-
CC         galactose 6-phosphate; Xref=Rhea:RHEA:24568, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:58534, ChEBI:CHEBI:91004; EC=3.2.1.85;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01574};
CC   -!- PATHWAY: Carbohydrate metabolism; lactose degradation; D-galactose 6-
CC       phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
CC       {ECO:0000255|HAMAP-Rule:MF_01574}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01574}.
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DR   EMBL; AE007317; AAK99228.1; -; Genomic_DNA.
DR   PIR; H97924; H97924.
DR   RefSeq; NP_358018.1; NC_003098.1.
DR   RefSeq; WP_000429432.1; NC_003098.1.
DR   AlphaFoldDB; Q8DQZ1; -.
DR   SMR; Q8DQZ1; -.
DR   STRING; 171101.spr0424; -.
DR   CAZy; GH1; Glycoside Hydrolase Family 1.
DR   PRIDE; Q8DQZ1; -.
DR   EnsemblBacteria; AAK99228; AAK99228; spr0424.
DR   GeneID; 60234162; -.
DR   KEGG; spr:spr0424; -.
DR   PATRIC; fig|171101.6.peg.466; -.
DR   eggNOG; COG2723; Bacteria.
DR   HOGENOM; CLU_001859_1_3_9; -.
DR   OMA; VEQWITL; -.
DR   UniPathway; UPA00542; UER00605.
DR   Proteomes; UP000000586; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0033920; F:6-phospho-beta-galactosidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR   GO; GO:0016052; P:carbohydrate catabolic process; IBA:GO_Central.
DR   GO; GO:0019512; P:lactose catabolic process via tagatose-6-phosphate; IEA:InterPro.
DR   HAMAP; MF_01574; LacG; 1.
DR   InterPro; IPR005928; 6P-beta-galactosidase.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; PTHR10353; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 1.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   TIGRFAMs; TIGR01233; lacG; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..470
FT                   /note="6-phospho-beta-galactosidase 1"
FT                   /id="PRO_0000260732"
FT   ACT_SITE        164
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT   ACT_SITE        378
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
SQ   SEQUENCE   470 AA;  54675 MW;  CAE61D8921067D6D CRC64;
     MENLQVKALP KEFLLGTATA AYQVEGATRV DGKGINMWDV YLQENSPFLP DPASDFYYRY
     EEDIALAAEH GLQALRLSIS WVRIFPDIDG DANVLAVHYY HRVFQSCLKH NVIPFVSLHH
     FDSPQKMLET GDWLNRENID RFIRYARFCF QEFTEVKHWF TINELMSLAA GQYIGGQFPP
     NHHFQLSEAI QANHNMLLAH ALAVLEFHQL GIEGKVGCIH ALKPGYPIDG QKENILAAKR
     YDVYNNKFLL DGTFLGYYSE DTLFHLNQIL EANNSSFIIE DGDLEIMKRA APLNTMFVMN
     YYRSEFIREY KGENRQEFNS TGIKGQSSFK LNALGEFVKK PGIPTTDWDW NIYPQGLFDM
     LLRIKEEYPQ HPVIYLTENG TALKEVKPEG ENDIIDDSKR IRYIEQHLHK VLEARDRGVN
     IQGYFIWSLQ DQFSWANGYN KRYGLFFVDY ETQKRYIKKS ALWVKGLKRN