ID   LACG_CLOAB              Reviewed;         474 AA.
AC   Q97EZ2;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=6-phospho-beta-galactosidase {ECO:0000255|HAMAP-Rule:MF_01574};
DE            EC=3.2.1.85 {ECO:0000255|HAMAP-Rule:MF_01574};
DE   AltName: Full=Beta-D-phosphogalactoside galactohydrolase {ECO:0000255|HAMAP-Rule:MF_01574};
DE            Short=PGALase {ECO:0000255|HAMAP-Rule:MF_01574};
DE   AltName: Full=P-beta-Gal {ECO:0000255|HAMAP-Rule:MF_01574};
DE            Short=PBG {ECO:0000255|HAMAP-Rule:MF_01574};
GN   Name=lacG {ECO:0000255|HAMAP-Rule:MF_01574}; OrderedLocusNames=CA_C2963;
OS   Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS   / VKM B-1787).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=272562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=11466286; DOI=10.1128/jb.183.16.4823-4838.2001;
RA   Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA   Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA   Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA   Smith D.R.;
RT   "Genome sequence and comparative analysis of the solvent-producing
RT   bacterium Clostridium acetobutylicum.";
RL   J. Bacteriol. 183:4823-4838(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 6-phospho-beta-D-galactoside + H2O = an alcohol + D-
CC         galactose 6-phosphate; Xref=Rhea:RHEA:24568, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:30879, ChEBI:CHEBI:58534, ChEBI:CHEBI:91004; EC=3.2.1.85;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01574};
CC   -!- PATHWAY: Carbohydrate metabolism; lactose degradation; D-galactose 6-
CC       phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
CC       {ECO:0000255|HAMAP-Rule:MF_01574}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01574}.
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DR   EMBL; AE001437; AAK80905.1; -; Genomic_DNA.
DR   PIR; F97264; F97264.
DR   RefSeq; NP_349565.1; NC_003030.1.
DR   RefSeq; WP_010966246.1; NC_003030.1.
DR   AlphaFoldDB; Q97EZ2; -.
DR   SMR; Q97EZ2; -.
DR   STRING; 272562.CA_C2963; -.
DR   CAZy; GH1; Glycoside Hydrolase Family 1.
DR   PRIDE; Q97EZ2; -.
DR   EnsemblBacteria; AAK80905; AAK80905; CA_C2963.
DR   GeneID; 44999451; -.
DR   KEGG; cac:CA_C2963; -.
DR   PATRIC; fig|272562.8.peg.3147; -.
DR   eggNOG; COG2723; Bacteria.
DR   HOGENOM; CLU_001859_1_3_9; -.
DR   OMA; MGAPGYT; -.
DR   OrthoDB; 654705at2; -.
DR   UniPathway; UPA00542; UER00605.
DR   Proteomes; UP000000814; Chromosome.
DR   GO; GO:0033920; F:6-phospho-beta-galactosidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0019512; P:lactose catabolic process via tagatose-6-phosphate; IEA:InterPro.
DR   HAMAP; MF_01574; LacG; 1.
DR   InterPro; IPR005928; 6P-beta-galactosidase.
DR   InterPro; IPR001360; Glyco_hydro_1.
DR   InterPro; IPR018120; Glyco_hydro_1_AS.
DR   InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR10353; PTHR10353; 1.
DR   Pfam; PF00232; Glyco_hydro_1; 1.
DR   PRINTS; PR00131; GLHYDRLASE1.
DR   SUPFAM; SSF51445; SSF51445; 1.
DR   TIGRFAMs; TIGR01233; lacG; 1.
DR   PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
DR   PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..474
FT                   /note="6-phospho-beta-galactosidase"
FT                   /id="PRO_0000260722"
FT   ACT_SITE        160
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT   ACT_SITE        374
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
SQ   SEQUENCE   474 AA;  54672 MW;  5661CA39AAC5C3BE CRC64;
     MNNFGEDFIF GGATAAYQAE GATKEDGKGP CIWDEYLKKE GRFTGDTASD FYHKYKEDLK
     FSRKFGVNGI RISIAWSRVI PDGKGEVNPK GLKFYSDLID ECIKNNVEPF VTLHHFDTPL
     TLFKDGDWLN RNNIDYFVRF AKVCFEALGD RVKKWITFNE AWAVAQNGYI IGNFPPSIKY
     DIPKAAQSMH NMMVAHAKVV ELYKSMNLDG EIGIVHTLEG KYPITDSKED KEAAYLDYMI
     SNKFMLDACF KGEYPKETEK TINEIMSKNG GELKIYDGDL EVLKKASSKI DFLGMNYYSS
     HFLKAYEGES RIHHNGTGEK GTSIFALKGI GERVNNPEVP TTDWDWPIYP KGLHDMLVRI
     KNEYPNYKKI YVTENGMGYK DDFKDGKIDD TPRIDYINKH LEAILKAKNE GVVVKGYFVW
     SLMDVLSWSN GYNKRYGLFY VDFKTQNRYA KKSAYWFKRI SYEKKLIDFS DIEY