LACG_LACCA
ID LACG_LACCA Reviewed; 474 AA.
AC P14696;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=6-phospho-beta-galactosidase {ECO:0000255|HAMAP-Rule:MF_01574};
DE EC=3.2.1.85 {ECO:0000255|HAMAP-Rule:MF_01574};
DE AltName: Full=Beta-D-phosphogalactoside galactohydrolase {ECO:0000255|HAMAP-Rule:MF_01574};
DE Short=PGALase {ECO:0000255|HAMAP-Rule:MF_01574};
DE AltName: Full=P-beta-Gal {ECO:0000255|HAMAP-Rule:MF_01574};
DE Short=PBG {ECO:0000255|HAMAP-Rule:MF_01574};
GN Name=lacG {ECO:0000255|HAMAP-Rule:MF_01574};
OS Lactobacillus casei.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lacticaseibacillus.
OX NCBI_TaxID=1582;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3130295; DOI=10.1016/0378-1119(88)90564-1;
RA Porter E.V., Chassy B.M.;
RT "Nucleotide sequence of the beta-D-phosphogalactoside galactohydrolase gene
RT of Lactobacillus casei: comparison to analogous pbg genes of other Gram-
RT positive organisms.";
RL Gene 62:263-276(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 393 / DSM 20011 / BCRC 10697 / JCM 1134 / NBRC 15883 / NCIMB
RC 11970 / NCDO 161 / WDCM 00100;
RX PubMed=9066115; DOI=10.1111/j.1574-6968.1997.tb10271.x;
RA Gosalbes M.J., Monedero V., Alpert C.-A., Perez-Martinez G.;
RT "Establishing a model to study the regulation of the lactose operon in
RT Lactobacillus casei.";
RL FEMS Microbiol. Lett. 148:83-89(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 384-474.
RX PubMed=3130296; DOI=10.1016/0378-1119(88)90565-3;
RA Alpert C.-A., Chassy B.M.;
RT "Molecular cloning and nucleotide sequence of the factor IIIlac gene of
RT Lactobacillus casei.";
RL Gene 62:277-288(1988).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 6-phospho-beta-D-galactoside + H2O = an alcohol + D-
CC galactose 6-phosphate; Xref=Rhea:RHEA:24568, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:30879, ChEBI:CHEBI:58534, ChEBI:CHEBI:91004; EC=3.2.1.85;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01574};
CC -!- PATHWAY: Carbohydrate metabolism; lactose degradation; D-galactose 6-
CC phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
CC {ECO:0000255|HAMAP-Rule:MF_01574}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC {ECO:0000255|HAMAP-Rule:MF_01574}.
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DR EMBL; M20151; AAD15134.1; -; Genomic_DNA.
DR EMBL; Z80834; CAB02557.1; -; Genomic_DNA.
DR EMBL; M20150; AAA25238.1; ALT_SEQ; Genomic_DNA.
DR PIR; A29897; A29897.
DR PIR; A29898; A29898.
DR AlphaFoldDB; P14696; -.
DR SMR; P14696; -.
DR STRING; 1582.AAW28_06695; -.
DR CAZy; GH1; Glycoside Hydrolase Family 1.
DR eggNOG; COG2723; Bacteria.
DR SABIO-RK; P14696; -.
DR UniPathway; UPA00542; UER00605.
DR GO; GO:0033920; F:6-phospho-beta-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019512; P:lactose catabolic process via tagatose-6-phosphate; IEA:InterPro.
DR HAMAP; MF_01574; LacG; 1.
DR InterPro; IPR005928; 6P-beta-galactosidase.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR018120; Glyco_hydro_1_AS.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10353; PTHR10353; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; SSF51445; 1.
DR TIGRFAMs; TIGR01233; lacG; 1.
DR PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 3: Inferred from homology;
KW Glycosidase; Hydrolase.
FT CHAIN 1..474
FT /note="6-phospho-beta-galactosidase"
FT /id="PRO_0000063882"
FT ACT_SITE 160
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
FT ACT_SITE 375
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01574"
SQ SEQUENCE 474 AA; 53982 MW; 1FF1B185E3549A9F CRC64;
MSKQLPQDFV MGGATAAYQV EGATKEDGKG RVLWDDFLDK QGRFKPDPAA DFYHRYDEDL
ALAEKYGHQV IRVSIAWSRI FPDGAGEVEP RGVAFYHKLF ADCAAHHIEP FVTLHHFDTP
ERLHEAGDWL SQEMLDDFVA YAKFCFEEFS EVKYWITINE PTSMAVQQYT TGTFPPAESG
RFDKTFQAEH NQMVAHARIV NLYKSMQLGG QIGIVHALQT VYPYSDSAVD HHAAELQDAL
ENRLYLDGTL AGEYHQETLA LVKEILDANH QPMFQSTPQE MKAIDEAAHQ LDFVGVNNYF
SKWLRAYHGK SETIHNGDGT KGSSVARLQG VGEEKLPDGI ETTDWDWSIY PRGMYDILMR
IHNDYPLVPV TYVTENGIGL KESLPENATP DTVIEDPKRI DYVKKYLSAM ADAIHDGANV
KGYFIWSLQD QFSWTNGYSK RYGLFFVDFP TQNRYIKQSA EWFKSVSETH IIPD
