ARCD_HALS3
ID ARCD_HALS3 Reviewed; 485 AA.
AC B0R9X2;
DT 27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Arginine/ornithine antiporter ArcD {ECO:0000303|PubMed:18930051};
GN Name=arcD {ECO:0000303|PubMed:18930051}; OrderedLocusNames=OE_5204R;
OS Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1).
OG Plasmid PHS3.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Halobacteriaceae; Halobacterium.
OX NCBI_TaxID=478009;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29341 / DSM 671 / R1;
RX PubMed=18313895; DOI=10.1016/j.ygeno.2008.01.001;
RA Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K.,
RA Ruepp A., Soppa J., Tittor J., Oesterhelt D.;
RT "Evolution in the laboratory: the genome of Halobacterium salinarum strain
RT R1 compared to that of strain NRC-1.";
RL Genomics 91:335-346(2008).
RN [2]
RP PROTEIN SEQUENCE OF 2-6; 178-200; 268-291; 293-307; 359-395 AND 397-410,
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, CATALYTIC ACTIVITY, AND
RP SUBCELLULAR LOCATION.
RC STRAIN=ATCC 29341 / DSM 671 / R1;
RX PubMed=18930051; DOI=10.1016/j.febslet.2008.10.004;
RA Wimmer F., Oberwinkler T., Bisle B., Tittor J., Oesterhelt D.;
RT "Identification of the arginine/ornithine antiporter ArcD from
RT Halobacterium salinarum.";
RL FEBS Lett. 582:3771-3775(2008).
CC -!- FUNCTION: Uptake of arginine from the medium in exchange for ornithine.
CC {ECO:0000269|PubMed:18930051}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-arginine(out) + L-ornithine(in) = L-arginine(in) + L-
CC ornithine(out); Xref=Rhea:RHEA:34991, ChEBI:CHEBI:32682,
CC ChEBI:CHEBI:46911; Evidence={ECO:0000269|PubMed:18930051};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:34992;
CC Evidence={ECO:0000269|PubMed:18930051};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18930051};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18930051}.
CC -!- SIMILARITY: Belongs to the NhaC Na(+)/H(+) (TC 2.A.35) antiporter
CC family. {ECO:0000305}.
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DR EMBL; AM774418; CAP15553.1; -; Genomic_DNA.
DR RefSeq; WP_010904158.1; NC_010368.1.
DR AlphaFoldDB; B0R9X2; -.
DR TCDB; 2.A.35.1.4; the nhac na(+):h(+) antiporter (nhac) family.
DR EnsemblBacteria; CAP15553; CAP15553; OE_5204R.
DR GeneID; 5954935; -.
DR GeneID; 62888213; -.
DR KEGG; hsl:OE_5204R; -.
DR HOGENOM; CLU_033405_1_0_2; -.
DR OMA; AFFNLVC; -.
DR PhylomeDB; B0R9X2; -.
DR Proteomes; UP000001321; Plasmid PHS3.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR InterPro; IPR018461; Na/H_Antiport_NhaC-like_C.
DR Pfam; PF03553; Na_H_antiporter; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Antiport; Cell membrane; Direct protein sequencing;
KW Membrane; Plasmid; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..485
FT /note="Arginine/ornithine antiporter ArcD"
FT /id="PRO_0000411687"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 441..461
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 485 AA; 50523 MW; 6FAC7AE066601350 CRC64;
MVEFEPRSYE DFDPEKRPSF GQALLPIAGM ITFLAVGIVL LGLDAQMPLL WGIAFTGVIA
RYGWGYTWDE LFDGISNSIV MGLGAIFILF IIYMLIASWV DAGTIPFIMY WGLEFLTPAV
FVPLAALLSF VVATAIGSSW TTAGSLGIAL VGIGSGLGIP APLTAGAILS GVYMGDKQSP
LSDTTLLASG VSDVDLWDHV RGMFPNTIIV GVISLALYAV LGLMAETGGT GGTGAEVAQI
QGGLAGTYTL SVLVLLPLVI TFGLAIKGYP ALPSLGAGVF SAAGVSILIQ GRGFAEAWQI
IYSGTGPKTG VDLVNNLLST GGLEGSIWVI TIVFGALSIG GILEATGVLS VIAHNTAKAV
DSVGGATLVT ALGPLVINAL TADQYMSIVI PGMTFRDLND EYDLDGTSLS RTLEETGTVS
EPMIPWNSGG VFMASALGVP VLSYLPYYFV GILSPILVVI MGFTGWKMYM KDPEESPEES
ADTAA