LACRT_HUMAN
ID LACRT_HUMAN Reviewed; 138 AA.
AC Q9GZZ8;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Extracellular glycoprotein lacritin;
DE Flags: Precursor;
GN Name=LACRT;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC TISSUE=Lacrimal gland;
RX PubMed=11419941; DOI=10.1006/jmbi.2001.4748;
RA Sanghi S., Kumar R., Lumsden A., Dickinson D., Klepeis V.,
RA Trinkaus-Randall V., Frierson H.F. Jr., Laurie G.W.;
RT "cDNA and genomic cloning of lacritin, a novel secretion enhancing factor
RT from the human lacrimal gland.";
RL J. Mol. Biol. 310:127-139(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thyroid;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 20-47 AND 87-138, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC TISSUE=Tear;
RX PubMed=25946035; DOI=10.1021/acs.jproteome.5b00179;
RA Azkargorta M., Soria J., Ojeda C., Guzman F., Acera A., Iloro I.,
RA Suarez T., Elortza F.;
RT "Human basal tear peptidome characterization by CID, HCD, and ETD followed
RT by in silico and in vitro analyses for antimicrobial peptide
RT identification.";
RL J. Proteome Res. 14:2649-2658(2015).
RN [4]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-119.
RC TISSUE=Saliva;
RX PubMed=16740002; DOI=10.1021/pr050492k;
RA Ramachandran P., Boontheung P., Xie Y., Sondej M., Wong D.T., Loo J.A.;
RT "Identification of N-linked glycoproteins in human saliva by glycoprotein
RT capture and mass spectrometry.";
RL J. Proteome Res. 5:1493-1503(2006).
CC -!- FUNCTION: Modulates secretion by lacrimal acinar cells.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed in secretory granules of many acinar
CC cells in lacrimal gland and in scattered acinar cells of salivary
CC glands.
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DR EMBL; AY005150; AAG32949.1; -; Genomic_DNA.
DR EMBL; AF238867; AAG44392.1; -; mRNA.
DR EMBL; BC062217; AAH62217.1; -; mRNA.
DR EMBL; BC069317; AAH69317.1; -; mRNA.
DR CCDS; CCDS8883.1; -.
DR RefSeq; NP_150593.1; NM_033277.1.
DR AlphaFoldDB; Q9GZZ8; -.
DR SMR; Q9GZZ8; -.
DR BioGRID; 124659; 116.
DR IntAct; Q9GZZ8; 42.
DR STRING; 9606.ENSP00000257867; -.
DR GlyGen; Q9GZZ8; 2 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q9GZZ8; -.
DR PhosphoSitePlus; Q9GZZ8; -.
DR BioMuta; LACRT; -.
DR DMDM; 33301325; -.
DR MassIVE; Q9GZZ8; -.
DR MaxQB; Q9GZZ8; -.
DR PaxDb; Q9GZZ8; -.
DR PeptideAtlas; Q9GZZ8; -.
DR PRIDE; Q9GZZ8; -.
DR ProteomicsDB; 80188; -.
DR Antibodypedia; 43570; 33 antibodies from 12 providers.
DR DNASU; 90070; -.
DR Ensembl; ENST00000257867.5; ENSP00000257867.4; ENSG00000135413.9.
DR GeneID; 90070; -.
DR KEGG; hsa:90070; -.
DR MANE-Select; ENST00000257867.5; ENSP00000257867.4; NM_033277.2; NP_150593.1.
DR UCSC; uc001sgi.2; human.
DR CTD; 90070; -.
DR DisGeNET; 90070; -.
DR GeneCards; LACRT; -.
DR HGNC; HGNC:16430; LACRT.
DR HPA; ENSG00000135413; Tissue enriched (salivary).
DR MIM; 607360; gene.
DR neXtProt; NX_Q9GZZ8; -.
DR OpenTargets; ENSG00000135413; -.
DR PharmGKB; PA30261; -.
DR VEuPathDB; HostDB:ENSG00000135413; -.
DR eggNOG; ENOG502TDZH; Eukaryota.
DR GeneTree; ENSGT00940000163391; -.
DR HOGENOM; CLU_1864409_0_0_1; -.
DR InParanoid; Q9GZZ8; -.
DR OMA; RAGKGMH; -.
DR OrthoDB; 1557381at2759; -.
DR PhylomeDB; Q9GZZ8; -.
DR TreeFam; TF340896; -.
DR PathwayCommons; Q9GZZ8; -.
DR SignaLink; Q9GZZ8; -.
DR BioGRID-ORCS; 90070; 10 hits in 1062 CRISPR screens.
DR ChiTaRS; LACRT; human.
DR GeneWiki; Lacritin; -.
DR GenomeRNAi; 90070; -.
DR Pharos; Q9GZZ8; Tbio.
DR PRO; PR:Q9GZZ8; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; Q9GZZ8; protein.
DR Bgee; ENSG00000135413; Expressed in lacrimal gland and 21 other tissues.
DR ExpressionAtlas; Q9GZZ8; baseline and differential.
DR Genevisible; Q9GZZ8; HS.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; HDA:UniProtKB.
DR GO; GO:0030141; C:secretory granule; IDA:UniProtKB.
DR GO; GO:0005518; F:collagen binding; IDA:UniProtKB.
DR GO; GO:0001968; F:fibronectin binding; IDA:UniProtKB.
DR GO; GO:0008083; F:growth factor activity; IDA:UniProtKB.
DR GO; GO:0043237; F:laminin-1 binding; IDA:UniProtKB.
DR GO; GO:0047485; F:protein N-terminus binding; IPI:UniProtKB.
DR GO; GO:0033173; P:calcineurin-NFAT signaling cascade; IDA:UniProtKB.
DR GO; GO:0019722; P:calcium-mediated signaling; IDA:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IDA:FlyBase.
DR GO; GO:0010669; P:epithelial structure maintenance; IDA:FlyBase.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IDA:FlyBase.
DR GO; GO:0031665; P:negative regulation of lipopolysaccharide-mediated signaling pathway; IDA:FlyBase.
DR GO; GO:0070886; P:positive regulation of calcineurin-NFAT signaling cascade; IDA:UniProtKB.
DR GO; GO:0050850; P:positive regulation of calcium-mediated signaling; IDA:FlyBase.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:UniProtKB.
DR GO; GO:0050679; P:positive regulation of epithelial cell proliferation; IDA:UniProtKB.
DR GO; GO:0060054; P:positive regulation of epithelial cell proliferation involved in wound healing; IDA:FlyBase.
DR GO; GO:0016239; P:positive regulation of macroautophagy; IDA:FlyBase.
DR GO; GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IDA:UniProtKB.
DR GO; GO:0051281; P:positive regulation of release of sequestered calcium ion into cytosol; IDA:UniProtKB.
DR GO; GO:0051047; P:positive regulation of secretion; IDA:UniProtKB.
DR GO; GO:0006473; P:protein acetylation; IMP:FlyBase.
DR GO; GO:0034067; P:protein localization to Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0070075; P:tear secretion; IDA:FlyBase.
DR InterPro; IPR043557; Dermcidin/Lacritin.
DR InterPro; IPR033530; Lacritin.
DR PANTHER; PTHR40711; PTHR40711; 1.
DR PANTHER; PTHR40711:SF2; PTHR40711:SF2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000269|PubMed:25946035"
FT CHAIN 20..138
FT /note="Extracellular glycoprotein lacritin"
FT /id="PRO_0000021574"
FT REGION 20..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 26..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 119
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:16740002"
SQ SEQUENCE 138 AA; 14246 MW; 09910581E650F6FF CRC64;
MKFTTLLFLA AVAGALVYAE DASSDSTGAD PAQEAGTSKP NEEISGPAEP ASPPETTTTA
QETSAAAVQG TAKVTSSRQE LNPLKSIVEK SILLTEQALA KAGKGMHGGV PGGKQFIENG
SEFAQKLLKK FSLLKPWA
