ID   LACR_LACLL              Reviewed;         255 AA.
AC   P18816;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Lactose phosphotransferase system repressor;
GN   Name=lacR;
OS   Lactococcus lactis subsp. lactis (Streptococcus lactis).
OG   Plasmid pLP712.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MG1820;
RX   PubMed=2120234; DOI=10.1016/s0021-9258(17)44779-x;
RA   van Rooijen R.J., de Vos W.M.;
RT   "Molecular cloning, transcriptional analysis, and nucleotide sequence of
RT   lacR, a gene encoding the repressor of the lactose phosphotransferase
RT   system of Lactococcus lactis.";
RL   J. Biol. Chem. 265:18499-18503(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-13.
RC   STRAIN=MG1820;
RX   PubMed=1901863; DOI=10.1016/s0021-9258(20)89626-4;
RA   van Rooijen R.J., van Schalkwijk S., de Vos W.M.;
RT   "Molecular cloning, characterization, and nucleotide sequence of the
RT   tagatose 6-phosphate pathway gene cluster of the lactose operon of
RT   Lactococcus lactis.";
RL   J. Biol. Chem. 266:7176-7181(1991).
CC   -!- FUNCTION: Repressor of the lactose catabolism operon. Galactose-6-
CC       phosphate is the inducer.
CC   -!- MISCELLANEOUS: This gene was sequenced from pMG820, a laboratory-
CC       derived deletion of the naturally occurring plasmid pLP712.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA25174.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAA25176.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAA25186.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M60673; AAA25186.1; ALT_INIT; Genomic_DNA.
DR   EMBL; M35375; AAA25175.1; -; Genomic_DNA.
DR   EMBL; M35375; AAA25174.1; ALT_INIT; Genomic_DNA.
DR   EMBL; M60447; AAA25176.1; ALT_INIT; Genomic_DNA.
DR   EMBL; M65190; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A36098; A36098.
DR   PIR; E39778; E39778.
DR   AlphaFoldDB; P18816; -.
DR   SMR; P18816; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0005988; P:lactose metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR014036; DeoR_C.
DR   InterPro; IPR001034; DeoR_HTH.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   InterPro; IPR018356; Tscrpt_reg_HTH_DeoR_CS.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00455; DeoRC; 1.
DR   Pfam; PF08220; HTH_DeoR; 1.
DR   PRINTS; PR00037; HTHLACR.
DR   SMART; SM00420; HTH_DEOR; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00894; HTH_DEOR_1; 1.
DR   PROSITE; PS51000; HTH_DEOR_2; 1.
PE   4: Predicted;
KW   DNA-binding; Lactose metabolism; Plasmid; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..255
FT                   /note="Lactose phosphotransferase system repressor"
FT                   /id="PRO_0000050256"
FT   DOMAIN          3..58
FT                   /note="HTH deoR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00349"
FT   DNA_BIND        20..39
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00349"
SQ   SEQUENCE   255 AA;  28617 MW;  76DCE6220D167D8B CRC64;
     MNKKRRLEKI LDMLKIDGTI TIKEIIDELD ISDMTARRDL DALEADGLLT RTHGGAQLLS
     SKKPLEKTHI EKKSLNTKEK IDIAKKACSL IKDGDTIFIG PGTTLVQLAL ELKGRKGYKI
     RVITNSLPVF LILNDSETID LLLLGGEYRE ITGAFVGSMA STNLKAMRFA KAFVRANAVT
     HNSIATYSDK EGVIQQLALN NAVEKFLLVD STKFDRYDFF NFYNLDQLDT IITDNQISPQ
     HLEEFSQYTT ILKAD