ID   LACS_LACSL              Reviewed;          68 AA.
AC   P23826; Q48848; Q48858;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 2.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Bacteriocin lactocin-S;
DE   Flags: Precursor;
GN   Name=lasA;
OS   Latilactobacillus sakei L45 (Lactobacillus sakei).
OG   Plasmid pCIM1.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Latilactobacillus.
OX   NCBI_TaxID=1634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEHYDRATION AT THR-33, D-AMINO ACID AT
RP   SER-38; SER-42 AND SER-50, LANTHIONINE CROSS-LINKS, PARTIAL PROTEIN
RP   SEQUENCE, AND PYRUVATE FORMATION AT SER-32.
RX   PubMed=7961627; DOI=10.1016/s0021-9258(18)46966-9;
RA   Skaugen M., Nissen-Meyer J., Jung G., Stevanovic S., Sletten K., Inger C.,
RA   Abildgaard C.I., Nes I.F.;
RT   "In vivo conversion of L-serine to D-alanine in a ribosomally synthesized
RT   polypeptide.";
RL   J. Biol. Chem. 269:27183-27185(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Skaugen M., Abildgaard C.I., Nes I.F.;
RT   "Analysis of genes involved in the biosynthesis of the lantibiotic lactocin
RT   S.";
RL   Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 43-67.
RX   PubMed=1872611; DOI=10.1128/aem.57.6.1829-1834.1991;
RA   Mortvedt C.I., Nissen-Meyer J., Sletten K., Nes I.F.;
RT   "Purification and amino acid sequence of lactocin S, a bacteriocin produced
RT   by Lactobacillus sake L45.";
RL   Appl. Environ. Microbiol. 57:1829-1834(1991).
CC   -!- FUNCTION: Inhibits the growth of selected species of the genera
CC       Lactobacillus, Pedicoccus, and Leuconostoc.
CC   -!- PTM: It is not established whether the 2,3-didehydrobutyrine is the
CC       E- or Z-isomer. {ECO:0000269|PubMed:7961627}.
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DR   EMBL; X79889; CAA56261.1; -; Genomic_DNA.
DR   EMBL; Z54312; CAA91109.1; -; Genomic_DNA.
DR   PIR; A55457; A55457.
DR   AlphaFoldDB; P23826; -.
DR   TCDB; 1.C.23.1.1; the lactocin s (lactocin s) family.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; D-amino acid;
KW   Direct protein sequencing; Lantibiotic; Plasmid; Pyruvate; Thioether bond.
FT   PROPEP          1..31
FT                   /id="PRO_0000002764"
FT   CHAIN           32..68
FT                   /note="Bacteriocin lactocin-S"
FT                   /id="PRO_0000002765"
FT   MOD_RES         32
FT                   /note="Pyruvic acid (Ser)"
FT                   /evidence="ECO:0000305|PubMed:7961627"
FT   MOD_RES         33
FT                   /note="2,3-didehydrobutyrine"
FT                   /evidence="ECO:0000269|PubMed:7961627"
FT   MOD_RES         38
FT                   /note="D-alanine (Ser)"
FT                   /evidence="ECO:0000269|PubMed:7961627"
FT   MOD_RES         42
FT                   /note="D-alanine (Ser)"
FT                   /evidence="ECO:0000269|PubMed:7961627"
FT   MOD_RES         50
FT                   /note="D-alanine (Ser)"
FT                   /evidence="ECO:0000269|PubMed:7961627"
FT   CROSSLNK        54..59
FT                   /note="Lanthionine (Ser-Cys)"
FT                   /evidence="ECO:0000269|PubMed:7961627"
FT   CROSSLNK        63..68
FT                   /note="Lanthionine (Ser-Cys)"
FT                   /evidence="ECO:0000269|PubMed:7961627"
SQ   SEQUENCE   68 AA;  7258 MW;  5FD203E8506ADC2C CRC64;
     MKTEKKVLDE LSLHASAKMG ARDVESSMNA DSTPVLASVA VSMELLPTAS VLYSDVAGCF
     KYSAKHHC