ID   LACT_LACCA              Reviewed;         292 AA.
AC   P24401;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Transcription antiterminator LacT;
GN   Name=lacT;
OS   Lactobacillus casei.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lacticaseibacillus.
OX   NCBI_TaxID=1582;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=64H;
RX   PubMed=9045813; DOI=10.1128/jb.179.5.1555-1562.1997;
RA   Alpert C.-A., Siebers U.;
RT   "The lac operon of Lactobacillus casei contains lacT, a gene coding for a
RT   protein of the BglG family of transcriptional antiterminators.";
RL   J. Bacteriol. 179:1555-1562(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 393 / DSM 20011 / BCRC 10697 / JCM 1134 / NBRC 15883 / NCIMB
RC   11970 / NCDO 161 / WDCM 00100;
RX   PubMed=9066115; DOI=10.1111/j.1574-6968.1997.tb10271.x;
RA   Gosalbes M.J., Monedero V., Alpert C.-A., Perez-Martinez G.;
RT   "Establishing a model to study the regulation of the lactose operon in
RT   Lactobacillus casei.";
RL   FEMS Microbiol. Lett. 148:83-89(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 218-292.
RX   PubMed=2125053; DOI=10.1016/s0021-9258(18)45742-0;
RA   Alpert C.-A., Chassy B.M.;
RT   "Molecular cloning and DNA sequence of lacE, the gene encoding the lactose-
RT   specific enzyme II of the phosphotransferase system of Lactobacillus casei.
RT   Evidence that a cysteine residue is essential for sugar phosphorylation.";
RL   J. Biol. Chem. 265:22561-22568(1990).
CC   -!- FUNCTION: Mediates positive regulation of the lac operon by functioning
CC       as an antiterminator factor of transcription.
CC   -!- SIMILARITY: Belongs to the transcriptional antiterminator BglG family.
CC       {ECO:0000305}.
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DR   EMBL; U21391; AAB49331.1; -; Genomic_DNA.
DR   EMBL; Z80834; CAB02555.1; -; Genomic_DNA.
DR   EMBL; M60851; AAA72983.1; -; Genomic_DNA.
DR   PIR; A23697; A23697.
DR   RefSeq; WP_003589800.1; NZ_MPOP01000080.1.
DR   AlphaFoldDB; P24401; -.
DR   SMR; P24401; -.
DR   STRING; 1582.AAW28_06685; -.
DR   eggNOG; COG3711; Bacteria.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.30.24.10; -; 1.
DR   InterPro; IPR004341; CAT_RNA-bd_dom.
DR   InterPro; IPR036650; CAT_RNA-bd_dom_sf.
DR   InterPro; IPR011608; PRD.
DR   InterPro; IPR036634; PRD_sf.
DR   InterPro; IPR001550; Transcrpt_antitermin_CS.
DR   Pfam; PF03123; CAT_RBD; 1.
DR   Pfam; PF00874; PRD; 2.
DR   SMART; SM01061; CAT_RBD; 1.
DR   SUPFAM; SSF50151; SSF50151; 1.
DR   SUPFAM; SSF63520; SSF63520; 2.
DR   PROSITE; PS00654; PRD_1; 1.
DR   PROSITE; PS51372; PRD_2; 2.
PE   3: Inferred from homology;
KW   Activator; Repeat; RNA-binding; Transcription; Transcription regulation.
FT   CHAIN           1..292
FT                   /note="Transcription antiterminator LacT"
FT                   /id="PRO_0000204246"
FT   DOMAIN          66..170
FT                   /note="PRD 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
FT   DOMAIN          172..284
FT                   /note="PRD 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00704"
SQ   SEQUENCE   292 AA;  33943 MW;  CC732DCE62E3D43C CRC64;
     MPKIAQIFNN NVALVDLDNR GQAVVRGRGI AFQKRRGDVI PTKQIEKIFY LANETSRQNL
     YFLLKNIPID VVTTTYEIID VAQKQYRLKV LDYIYITLSD HIYEAYKRYQ AGTYQETMVP
     DFHIQYPAEY AVAKQALQII ATNLGVQFPQ SEIKNLALHF INASGEDDGE QVFGKSNEAS
     LSQLVQEVLK RHHITRSHSN GNYYDRFMIH LQYLIDRLQR VDTYAVTIVP EVATELKQNY
     PQSYKIASEI FDEIKDQLYR SMSEDERLYF IIHIQRLINE APAQNHSQND SL