LACY_KLEOX
ID LACY_KLEOX Reviewed; 416 AA.
AC P18817;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 2.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Lactose permease;
DE AltName: Full=Lactose-proton symport;
GN Name=lacY;
OS Klebsiella oxytoca.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=571;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M5a1;
RX PubMed=3056524; DOI=10.1016/0005-2736(88)90494-4;
RA McMorrow I., Chin D.T., Fiebig K., Pierce J.L., Wilson D.M., Reeve E.C.R.,
RA Wilson T.H.;
RT "The lactose carrier of Klebsiella pneumoniae M5a1; the physiology of
RT transport and the nucleotide sequence of the lacY gene.";
RL Biochim. Biophys. Acta 945:315-323(1988).
CC -!- FUNCTION: Responsible for transport of beta-galactosides into the cell,
CC with the concomitant import of a proton (symport system).
CC {ECO:0000250|UniProtKB:P02920}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P02920}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P02920}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Oligosaccharide:H(+) symporter (OHS) (TC 2.A.1.5) family.
CC {ECO:0000305}.
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DR EMBL; X14154; CAA32366.1; -; Genomic_DNA.
DR AlphaFoldDB; P18817; -.
DR SMR; P18817; -.
DR STRING; 571.MC52_23405; -.
DR eggNOG; COG2223; Bacteria.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005351; F:carbohydrate:proton symporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR000576; LacY/RafB_perm_fam.
DR InterPro; IPR018457; LacY/RafB_perm_fam_CS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF01306; LacY_symp; 1.
DR PRINTS; PR00174; LACYSMPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00882; 2A0105; 1.
DR PROSITE; PS00896; LACY_1; 1.
DR PROSITE; PS00897; LACY_2; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Sugar transport; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..416
FT /note="Lactose permease"
FT /id="PRO_0000196185"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..50
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..80
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 103..123
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 124..149
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..223
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 245..267
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..295
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 338..353
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 375..384
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 385..405
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 406..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT SITE 131
FT /note="Substrate binding"
FT /evidence="ECO:0000250|UniProtKB:P02920"
FT SITE 149
FT /note="Substrate binding"
FT /evidence="ECO:0000250|UniProtKB:P02920"
FT SITE 274
FT /note="Substrate binding and proton translocation"
FT /evidence="ECO:0000250|UniProtKB:P02920"
FT SITE 307
FT /note="Proton translocation"
FT /evidence="ECO:0000250|UniProtKB:P02920"
FT SITE 327
FT /note="Proton translocation"
FT /evidence="ECO:0000250|UniProtKB:P02920"
FT SITE 330
FT /note="Proton translocation"
FT /evidence="ECO:0000250|UniProtKB:P02920"
SQ SEQUENCE 416 AA; 46221 MW; A37D8BA4C38C467B CRC64;
MKLSELAPRE RHNFIYFMLF FFFYYFIMSA YFPFFPVWLA EVNHLTKTET GIVFSCISLF
AIIFQPVFGL ISDKLGLRKH LLWTITILLI LFAPFFIFVF SPLLQMNIMA GALVGGVYLG
IVFSSRSGAV EAYIERVSRA NRFEYGKVRV SGCVGWALCA SITGILFSID PNITFWIASG
FALILGVLLW VSKPESSNSA EVIDALGANR QAFSMRTAAE LFRMPRFWGF IIYVVGVASV
YDVFDQQFAN FFKGFFSSPQ RGTEVFGFVT TGGELLNALI MFCAPAIINR IGAKNALLIA
GLIMSVRILG SSFATSAVEV IILKMLHMFE IPFLLVGTFK YISSAFKGKL SATLFLIGFN
LSKQLSSVVL SAWVGRMYDT VGFHQAYLIL GCITLSFTVI SLFTLKGSKT LLPATA
