ID   LACY_KLEPN              Reviewed;         131 AA.
AC   P59832;
DT   29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   29-AUG-2003, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Lactose permease;
DE   AltName: Full=Lactose-proton symport;
DE   Flags: Fragment;
GN   Name=lacY;
OS   Klebsiella pneumoniae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=T17R1;
RX   PubMed=3897196; DOI=10.1128/jb.163.3.850-857.1985;
RA   Buvinger W.E., Riley M.;
RT   "Nucleotide sequence of Klebsiella pneumoniae lac genes.";
RL   J. Bacteriol. 163:850-857(1985).
CC   -!- FUNCTION: Responsible for transport of beta-galactosides into the cell,
CC       with the concomitant import of a proton (symport system).
CC       {ECO:0000250|UniProtKB:P02920}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P02920}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P02920}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Oligosaccharide:H(+) symporter (OHS) (TC 2.A.1.5) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA25083.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M11441; AAA25083.1; ALT_INIT; Genomic_DNA.
DR   PIR; C24925; C24925.
DR   AlphaFoldDB; P59832; -.
DR   SMR; P59832; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005351; F:carbohydrate:proton symporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR000576; LacY/RafB_perm_fam.
DR   InterPro; IPR018457; LacY/RafB_perm_fam_CS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF01306; LacY_symp; 1.
DR   PRINTS; PR00174; LACYSMPORT.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS00896; LACY_1; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Sugar transport; Symport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..>131
FT                   /note="Lactose permease"
FT                   /id="PRO_0000196186"
FT   TOPO_DOM        1..13
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        35..50
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        72..80
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        81..101
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        102
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        103..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        124..131
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   NON_TER         131
SQ   SEQUENCE   131 AA;  14959 MW;  6CF2B78F7A6CD4FA CRC64;
     MKFSELAPRE RHNFVYFLLF FFFYHFIMSA YFPFFPVWLA DVNHLTKTET GIVFSSISLF
     AIIFQPVFGL MSDKLGLRKH LLWTITVLLI LFAPFFIFVF SPLLQMNIIA GSLVGGIYLG
     IVFSTAPGVG S