LACY_LACHE
ID LACY_LACHE Reviewed; 638 AA.
AC Q7WTB2;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Lactose permease;
DE AltName: Full=Lactose transport protein;
DE AltName: Full=Lactose transporter;
DE AltName: Full=Lactose-proton symporter;
DE Includes:
DE RecName: Full=Putative phosphotransferase enzyme IIA component;
DE EC=2.7.1.-;
DE AltName: Full=Putative PTS system EIIA component;
GN Name=lacS;
OS Lactobacillus helveticus (Lactobacillus suntoryeus).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lactobacillus.
OX NCBI_TaxID=1587;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TRANSCRIPTIONAL REGULATION.
RC STRAIN=ATCC 15009 / DSM 20075 / BCRC 12936 / JCM 1120 / NBRC 15019 / NCIMB
RC 11971 / NRRL B-4526 / Lh12;
RX PubMed=12788721; DOI=10.1128/aem.69.6.3238-3243.2003;
RA Fortina M.G., Ricci G., Mora D., Guglielmetti S., Manachini P.L.;
RT "Unusual organization for lactose and galactose gene clusters in
RT Lactobacillus helveticus.";
RL Appl. Environ. Microbiol. 69:3238-3243(2003).
CC -!- FUNCTION: Responsible for transport of beta-galactosides into the cell,
CC with the concomitant uptake of protons (symport system), and also for
CC transport of homologous and heterologous exchange of beta-galactosides.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: By lactose. {ECO:0000269|PubMed:12788721}.
CC -!- DOMAIN: The PTS EIIA type-1 domain may serve a regulatory function,
CC through its phosphorylation activity. {ECO:0000250}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the
CC sodium:galactoside symporter (TC 2.A.2) family. {ECO:0000305}.
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DR EMBL; AJ512878; CAD55501.1; -; Genomic_DNA.
DR RefSeq; WP_003627050.1; NZ_SCLV01000034.1.
DR AlphaFoldDB; Q7WTB2; -.
DR SMR; Q7WTB2; -.
DR STRING; 326425.lhe_1439; -.
DR GeneID; 66452191; -.
DR eggNOG; COG2190; Bacteria.
DR eggNOG; COG2211; Bacteria.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0006814; P:sodium ion transport; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR Gene3D; 2.70.70.10; -; 1.
DR InterPro; IPR011055; Dup_hybrid_motif.
DR InterPro; IPR039672; MFS_2.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR001927; Na/Gal_symport.
DR InterPro; IPR018043; Na/Gal_symport_CS.
DR InterPro; IPR001127; PTS_EIIA_1_perm.
DR PANTHER; PTHR11328; PTHR11328; 1.
DR Pfam; PF00358; PTS_EIIA_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR SUPFAM; SSF51261; SSF51261; 1.
DR TIGRFAMs; TIGR00792; gph; 1.
DR TIGRFAMs; TIGR00830; PTBA; 1.
DR PROSITE; PS00872; NA_GALACTOSIDE_SYMP; 1.
DR PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
DR PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Kinase; Membrane; Phosphoprotein; Sugar transport; Symport;
KW Transferase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..638
FT /note="Lactose permease"
FT /id="PRO_0000170758"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..449
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 503..610
FT /note="PTS EIIA type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00416"
FT REGION 1..470
FT /note="Permease"
FT MOD_RES 558
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000250"
SQ SEQUENCE 638 AA; 69669 MW; 7B00C98B7D8893D0 CRC64;
MHNHKVSGKQ IVSYASFCLG NLGHSAFYGV MSTYFIIFIT SGMFSGLNQS VADKLIGLIT
GLMVLVRIIE LVIDPILGNV VDNTKTRWGK FKPWILIGTV VSAALLLILF TGIFGLAQQN
WILFAILFVL IYIAFDVFYS LSDVSYWGMV PALSEDSHER GIYTSLGAFS GIIGWNSLPI
IVVPLVTGVT YAVTGKHEEG APGWFAFAAV ISALAIICAL IVCFGTKEKH NIIRDSAKQK
TTLRQVFGAI FHNDQILWPS LAYLLYSLAA VITNGVLFYM YKFVIGKPND FWVVGIIATI
IGCCINPSFP VLNKYIPRKW LFIAGQTCMV LAYVLFIFGH NNVFLMDLGL VLFNINFALL
VTVLTLTDAI EYGQLKIGQR NEAVVLAVRP MIDKFAGAVS NALVGYVAIA AGMTGSATAA
DMTSKGINTF NMMALYIPLA LAVLSIVVFS LKVTLSEKKH AQVIEELKSK LAQGEIEKKT
SVDTGTKEVT IYAPADGELM QMSSVVDEDG KPFPGKGFAI EPSSGQIYAP FDGTIKFTFG
TKHAFEIVSQ NGLQVVVHVG LGTVNLRGEG FETFYDDGQT VKKGDKLLEF DRDLALNNGY
KDTIVIFYTQ PGRIQNSGTI QAGKDIKHGE KVVDVQFK
