ID   LACY_LEULA              Reviewed;         639 AA.
AC   Q48624;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Lactose permease;
DE   AltName: Full=Lactose transport protein;
DE   AltName: Full=Lactose-proton symporter;
DE   Includes:
DE     RecName: Full=Putative phosphotransferase enzyme IIA component;
DE              EC=2.7.1.-;
DE     AltName: Full=Putative PTS system EIIA component;
GN   Name=lacS;
OS   Leuconostoc lactis.
OG   Plasmid pNZ63.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=1246;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NZ6009;
RX   PubMed=8633855; DOI=10.1128/aem.62.5.1574-1582.1996;
RA   Vaughan E.E., David S., de Vos W.M.;
RT   "The lactose transporter in Leuconostoc lactis is a new member of the LacS
RT   subfamily of galactoside-pentose-hexuronide translocators.";
RL   Appl. Environ. Microbiol. 62:1574-1582(1996).
CC   -!- FUNCTION: Responsible for transport of beta-galactosides into the cell,
CC       with the concomitant uptake of protons (symport system), and also for
CC       transport of homologous and heterologous exchange of beta-galactosides.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- DOMAIN: The PTS EIIA type-1 domain may serve a regulatory function,
CC       through its phosphorylation activity.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the
CC       sodium:galactoside symporter (TC 2.A.2) family. {ECO:0000305}.
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DR   EMBL; U47655; AAC44113.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q48624; -.
DR   SMR; Q48624; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0006814; P:sodium ion transport; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   Gene3D; 2.70.70.10; -; 1.
DR   InterPro; IPR011055; Dup_hybrid_motif.
DR   InterPro; IPR039672; MFS_2.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR001927; Na/Gal_symport.
DR   InterPro; IPR018043; Na/Gal_symport_CS.
DR   InterPro; IPR001127; PTS_EIIA_1_perm.
DR   PANTHER; PTHR11328; PTHR11328; 1.
DR   Pfam; PF00358; PTS_EIIA_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   SUPFAM; SSF51261; SSF51261; 1.
DR   TIGRFAMs; TIGR00792; gph; 1.
DR   TIGRFAMs; TIGR00830; PTBA; 1.
DR   PROSITE; PS00872; NA_GALACTOSIDE_SYMP; 1.
DR   PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
DR   PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Kinase; Membrane; Phosphoprotein; Plasmid; Sugar transport;
KW   Symport; Transferase; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..639
FT                   /note="Lactose permease"
FT                   /id="PRO_0000170759"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        264..284
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        294..314
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        322..342
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        347..367
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        398..418
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        433..453
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          505..609
FT                   /note="PTS EIIA type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00416"
FT   REGION          1..473
FT                   /note="Permease"
FT   MOD_RES         557
FT                   /note="Phosphohistidine; by HPr"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   639 AA;  70153 MW;  25DF28197761B415 CRC64;
     MKDITKQKFS RNKLVEMISF ALGNLGHAAF YGALSTYFIV YVTSGMFDGL PQSVANKLIG
     LITALVVIIR LAEVIIDPIL GNIVDNTKTR WGKFKPWQVI GAVVSSVLLV VIFTGIFGLA
     HINWIAFAIV FTVLFILLDI FYSFADVAYW GMVPAISEDS KERGIFTSLG SFTGSIGWNG
     LTMIVVPVTT YFTFIATGKH EQGPSGWFGF SIVVSIVAVL SALAVAFGTK EKDNLIRNAA
     TKKTSIKDVF SGIIHNDQIL WISLAYLMYS LAYVVTNGVL FYFFKFVLGK PNEFWIAGAI
     ATVIGFSTAP LYPVLNKFIT RKVLFSIGQM AMILSYLFFI FGKTNMMMVT IGLILFNFTF
     AQLVVVLSLT DSIEYGQLKN GNRNEAVVLA VRPMLDKITG AFSNGLVGAI AITAGMTGSA
     TAGDISASKI NTFEIYAFYT PLLFSILALV IFLWKVKITE KKHAEIVIEL EKTLSSGAKK
     ANTSEVNVEL EEIFAPASGQ KKLLNEVDGN TLTGIGFAID PEEGNLFAPF DGKVDFTFST
     KHVLGVVSNN GLKAIIHVGI GTINMRGAGF VSHYVDGQLF KKGDLLMTFD KKLITKNGYQ
     DDIIMYFTQP ENIIDVQQID NRVVKQGEKI AKLTFRSER