LACY_STRTR
ID LACY_STRTR Reviewed; 634 AA.
AC P23936;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-1992, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Lactose permease;
DE AltName: Full=Lactose transport protein;
DE AltName: Full=Lactose-proton symporter;
DE Includes:
DE RecName: Full=Putative phosphotransferase enzyme IIA component;
DE EC=2.7.1.-;
DE AltName: Full=Putative PTS system EIIA component;
GN Name=lacS;
OS Streptococcus thermophilus.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=1308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=A147;
RX PubMed=2644191; DOI=10.1128/jb.171.1.244-253.1989;
RA Poolman B., Royer T.J., Mainzer S.E., Schmidt B.F.;
RT "Lactose transport system of Streptococcus thermophilus: a hybrid protein
RT with homology to the melibiose carrier and enzyme III of
RT phosphoenolpyruvate-dependent phosphotransferase systems.";
RL J. Bacteriol. 171:244-253(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16.
RC STRAIN=A147;
RX PubMed=1694527; DOI=10.1128/jb.172.7.4037-4047.1990;
RA Poolman B., Royer T.J., Mainzer S.E., Schmidt B.F.;
RT "Carbohydrate utilization in Streptococcus thermophilus: characterization
RT of the genes for aldose 1-epimerase (mutarotase) and UDPglucose 4-
RT epimerase.";
RL J. Bacteriol. 172:4037-4047(1990).
CC -!- FUNCTION: Responsible for transport of beta-galactosides into the cell,
CC with the concomitant uptake of protons (symport system), and also for
CC transport of homologous and heterologous exchange of beta-galactosides.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- DOMAIN: The PTS EIIA type-1 domain may serve a regulatory function,
CC through its phosphorylation activity.
CC -!- SIMILARITY: In the N-terminal section; belongs to the
CC sodium:galactoside symporter (TC 2.A.2) family. {ECO:0000305}.
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DR EMBL; M23009; AAA26910.1; -; Genomic_DNA.
DR EMBL; M38175; AAA26946.1; -; Genomic_DNA.
DR PIR; A32241; A32241.
DR RefSeq; WP_014608519.1; NZ_QFFS01000004.1.
DR AlphaFoldDB; P23936; -.
DR SMR; P23936; -.
DR STRING; 322159.STER_1367; -.
DR TCDB; 2.A.2.2.1; the glycoside-pentoside-hexuronide (gph):cation symporter family.
DR eggNOG; COG2190; Bacteria.
DR eggNOG; COG2211; Bacteria.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0006814; P:sodium ion transport; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR Gene3D; 2.70.70.10; -; 1.
DR InterPro; IPR011055; Dup_hybrid_motif.
DR InterPro; IPR039672; MFS_2.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR001927; Na/Gal_symport.
DR InterPro; IPR018043; Na/Gal_symport_CS.
DR InterPro; IPR001127; PTS_EIIA_1_perm.
DR PANTHER; PTHR11328; PTHR11328; 1.
DR Pfam; PF00358; PTS_EIIA_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR SUPFAM; SSF51261; SSF51261; 1.
DR TIGRFAMs; TIGR00792; gph; 1.
DR TIGRFAMs; TIGR00830; PTBA; 1.
DR PROSITE; PS00872; NA_GALACTOSIDE_SYMP; 1.
DR PROSITE; PS51093; PTS_EIIA_TYPE_1; 1.
DR PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1.
PE 3: Inferred from homology;
KW Cell membrane; Kinase; Membrane; Phosphoprotein; Sugar transport; Symport;
KW Transferase; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..634
FT /note="Lactose permease"
FT /id="PRO_0000170760"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 500..604
FT /note="PTS EIIA type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00416"
FT REGION 1..490
FT /note="Permease"
FT MOD_RES 552
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000250"
SQ SEQUENCE 634 AA; 69454 MW; B1B095791D174CBE CRC64;
MEKSKGQMKS RLSYAAGAFG NDVFYATLST YFIMFVTTHL FNTGDPKQNS HYVLLITNII
SILRILEVFI DPLIGNMIDN TNTKYGKFKP WVVGGGIISS ITLLLLFTDL GGLNKTNPFL
YLVLFGIIYL VMDVFYSIKD IGFWSMIPAL SLDSHEREKM ATFARIGSTI GANIVGVAIM
PIVLFFSMTN NSGSGDKSGW FWFAFIVALI GVITSIAVGI GTREVESKIR DNNEKTSLKQ
VFKVLGQNDQ LMWLSLGYWF YGLGINTLNA LQLYYFTFIL GDSGKYSILY GLNTVVGLVS
VSLFPTLADK FNRKRLFYGC IAVMLGGIGI FSIAGTSLPI ILTAAELFFI PQPLVFLVVF
MIISDSVEYG QWKTGHRDES LTLSVRPLID KLGGAMSNWL VSTFAVAAGM TTGASASTIT
THQQFIFKLG MFAFPAATML IGAFIVARKI TLTEARHAKI VEELEHRFSV ATSENEVKAN
VVSLVTPTTG YLVDLSSVND EHFASGSMGK GFAIKPTDGA VFAPISGTIR QILPTRHAVG
IESEDGVIVL IHVGIGTVKL NGEGFISYVE QGDRVEVGQK LLEFWSPIIE KNGLDDTVLV
TVTNSEKFSA FHLEQKVGEK VEALSEVITF KKGE
