ID   LAC_SANBA               Reviewed;           8 AA.
AC   C0HJB2;
DT   01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2013, sequence version 1.
DT   29-SEP-2021, entry version 11.
DE   RecName: Full=Laccase {ECO:0000303|Ref.1};
DE            EC=1.10.3.2 {ECO:0000269|Ref.1};
DE   Flags: Fragment;
OS   Sanghuangporus baumii (Phellinus baumii).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Hymenochaetales; Hymenochaetaceae; Sanghuangporus.
OX   NCBI_TaxID=108892 {ECO:0000269|Ref.1};
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ACTIVITY
RP   REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION.
RC   STRAIN=MW0801 {ECO:0000269|Ref.1}; TISSUE=Mycelium {ECO:0000269|Ref.1};
RX   DOI=10.1016/j.molcatb.2013.10.004;
RA   Sun J., Chen Q.J., Zhu J.M., Wang H.X., Zhang G.Q.;
RT   "An extracellular laccase with antiproliferative activity from the
RT   sanghuang mushroom Inonotus baumii.";
RL   J. Mol. Catal., B Enzym. 99:20-25(2014).
CC   -!- FUNCTION: Lignin degradation and detoxification of lignin-derived
CC       products. Exhibits broad substrate specificity against a range of
CC       aromatic compounds with highest activity against guaiacol. Shows lower
CC       activity against 4-methylcatechol, 4-hydroxyindole, catechol,
CC       hydroquinone and 2,6-dimethoxy-phenol. {ECO:0000269|Ref.1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O;
CC         Xref=Rhea:RHEA:11276, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:17594, ChEBI:CHEBI:17977; EC=1.10.3.2;
CC         Evidence={ECO:0000269|Ref.1};
CC   -!- COFACTOR:
CC       Name=Cu(2+); Xref=ChEBI:CHEBI:29036; Evidence={ECO:0000269|Ref.1};
CC       Note=Binds 4 Cu cations per monomer. {ECO:0000250|UniProtKB:Q70KY3};
CC   -!- ACTIVITY REGULATION: Inhibited by Ca(2+), Fe(2+) and Al(3+).
CC       {ECO:0000269|Ref.1}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 2.4. Stable at pH 7.2 and 5.2. {ECO:0000269|Ref.1};
CC       Temperature dependence:
CC         Optimum temperature is 20 degrees Celsius. Thermostable, maintains
CC         activity from 50 to 60 degrees Celsius. {ECO:0000269|Ref.1};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- MISCELLANEOUS: Inhibits cell proliferation of HepG2 hepatoma cell line
CC       with an IC(50) of 2.6 uM and L1210 mouse leukemia cell line with an
CC       IC(50)=3.2 uM respectively. {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the multicopper oxidase family. {ECO:0000305}.
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DR   GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR   GO; GO:0052716; F:hydroquinone:oxygen oxidoreductase activity; IDA:UniProtKB.
DR   GO; GO:0046274; P:lignin catabolic process; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Lignin degradation; Oxidoreductase; Secreted.
FT   CHAIN           1..>8
FT                   /note="Laccase"
FT                   /id="PRO_0000422126"
FT   NON_TER         8
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   8 AA;  799 MW;  87CB1AB2D768705D CRC64;
     AIGPVDEV