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LAEA_GIBF5
ID   LAEA_GIBF5              Reviewed;         316 AA.
AC   S0DQI7; E0WDF6;
DT   16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT   16-MAR-2016, sequence version 2.
DT   03-AUG-2022, entry version 29.
DE   RecName: Full=Secondary metabolism regulator LAE1 {ECO:0000305};
DE   AltName: Full=Methyltransferase LAE1 {ECO:0000305};
DE            EC=2.1.1.- {ECO:0000250|UniProtKB:C8VQG9};
DE   AltName: Full=Velvet complex subunit LAE1 {ECO:0000305};
GN   Name=LAE1 {ECO:0000303|PubMed:20572938}; ORFNames=FFUJ_00592;
OS   Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae
OS   and foot rot disease fungus) (Fusarium fujikuroi).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC   Fusarium fujikuroi species complex.
OX   NCBI_TaxID=1279085;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, SUBCELLULAR LOCATION,
RP   INTERACTION WITH VEL1/VEA, AND DISRUPTION PHENOTYPE.
RC   STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831;
RX   PubMed=20572938; DOI=10.1111/j.1365-2958.2010.07263.x;
RA   Wiemann P., Brown D.W., Kleigrewe K., Bok J.W., Keller N.P., Humpf H.U.,
RA   Tudzynski B.;
RT   "FfVel1 and FfLae1, components of a velvet-like complex in Fusarium
RT   fujikuroi, affect differentiation, secondary metabolism and virulence.";
RL   Mol. Microbiol. 77:972-994(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831;
RX   PubMed=23825955; DOI=10.1371/journal.ppat.1003475;
RA   Wiemann P., Sieber C.M.K., von Bargen K.W., Studt L., Niehaus E.-M.,
RA   Espino J.J., Huss K., Michielse C.B., Albermann S., Wagner D.,
RA   Bergner S.V., Connolly L.R., Fischer A., Reuter G., Kleigrewe K., Bald T.,
RA   Wingfield B.D., Ophir R., Freeman S., Hippler M., Smith K.M., Brown D.W.,
RA   Proctor R.H., Muensterkoetter M., Freitag M., Humpf H.-U., Gueldener U.,
RA   Tudzynski B.;
RT   "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen
RT   Fusarium fujikuroi reveal complex regulation of secondary metabolism and
RT   novel metabolites.";
RL   PLoS Pathog. 9:E1003475-E1003475(2013).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24389666; DOI=10.1007/s00253-013-5453-1;
RA   Niehaus E.M., von Bargen K.W., Espino J.J., Pfannmueller A., Humpf H.U.,
RA   Tudzynski B.;
RT   "Characterization of the fusaric acid gene cluster in Fusarium fujikuroi.";
RL   Appl. Microbiol. Biotechnol. 98:1749-1762(2014).
CC   -!- FUNCTION: Methyltransferase that performs automethylation (By
CC       similarity). No other methyl-accepting substrate has been identified
CC       yet (By similarity). Component of the velvet transcription factor
CC       complex that acts as a global regulator for secondary metabolite gene
CC       expression (PubMed:20572938). Controls the expression of the
CC       gibberellins gene clusters, but does not affect bikaverin production
CC       (PubMed:20572938). Controls the expression of the fusaric acid gene
CC       cluster (PubMed:24389666). Acts as a virulence factors during
CC       infection, most likely through activation of gibberellins biosynthesis
CC       (PubMed:20572938). {ECO:0000250|UniProtKB:C8VQG9,
CC       ECO:0000269|PubMed:20572938, ECO:0000269|PubMed:24389666}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-methionyl-[protein] + S-adenosyl-L-methionine = S-adenosyl-
CC         L-homocysteine + S-methyl-L-methionyl-[protein];
CC         Xref=Rhea:RHEA:60560, Rhea:RHEA-COMP:12313, Rhea:RHEA-COMP:15592,
CC         ChEBI:CHEBI:16044, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:142742; Evidence={ECO:0000250|UniProtKB:C8VQG9};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60561;
CC         Evidence={ECO:0000250|UniProtKB:C8VQG9};
CC   -!- SUBUNIT: Component of the heterotrimeric velvet complex composed of
CC       LAE1, VEL1 and VEL2; VEL1 acting as a bridging protein between LAE1 and
CC       VEL2 (By similarity). Interacts with VEL1 (PubMed:20572938).
CC       {ECO:0000250|UniProtKB:C8VQG9, ECO:0000269|PubMed:20572938}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20572938}.
CC   -!- INDUCTION: Expression is regulated by the VEL1 (PubMed:20572938).
CC       {ECO:0000269|PubMed:20572938}.
CC   -!- DISRUPTION PHENOTYPE: Reduces gibberellins production and subsequent
CC       virulence during infection (PubMed:20572938). Reduces both the fusaric
CC       acid gene cluster expression and production of fusaric acid
CC       (PubMed:24389666). {ECO:0000269|PubMed:20572938,
CC       ECO:0000269|PubMed:24389666}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. LaeA
CC       methyltransferase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCT62818.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; FN548141; CBE54370.1; -; Genomic_DNA.
DR   EMBL; HF679023; CCT62818.1; ALT_SEQ; Genomic_DNA.
DR   AlphaFoldDB; S0DQI7; -.
DR   SMR; S0DQI7; -.
DR   STRING; 1279085.S0DQI7; -.
DR   EnsemblFungi; CCT62818; CCT62818; FFUJ_00592.
DR   HOGENOM; CLU_010595_2_0_1; -.
DR   Proteomes; UP000016800; Chromosome 1.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   1: Evidence at protein level;
KW   Methyltransferase; Nucleus; Reference proteome; S-adenosyl-L-methionine;
KW   Sporulation; Transcription; Transcription regulation; Transferase;
KW   Virulence.
FT   CHAIN           1..316
FT                   /note="Secondary metabolism regulator LAE1"
FT                   /id="PRO_0000435749"
SQ   SEQUENCE   316 AA;  36624 MW;  4657FCFE4FFDB2DA CRC64;
     MVVMPPQNSV NESEGRYLQD GFWQHGRFYG SWKPGKYLFP IDSEELNRLD IFHKVFLLAR
     DNKPFLAPIR RTSPRIMDIG TGTGIWAINV AEECLSDAQI MAVDLNQIQP ALIPPGFMPK
     QYDIEEPSWG PLLADCDLIH MRMLLGSIQT DLWPQVYHNA FEHLTPGIGF LEHIEVDWIP
     RCDDDERPAN SAFVKWAELF LDGMDRFNRS VRVIPQEHRQ MLEATGFTDV KQEVIKAYVC
     PWSADRNERE IARWFNIGLS HSLEAMSLKP LIEKLGFEAE DVRELCERAK RETCVLRYHT
     YCNIHVWTAR KPGPQQ
 
 
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