LAEA_GIBF5
ID LAEA_GIBF5 Reviewed; 316 AA.
AC S0DQI7; E0WDF6;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 16-MAR-2016, sequence version 2.
DT 03-AUG-2022, entry version 29.
DE RecName: Full=Secondary metabolism regulator LAE1 {ECO:0000305};
DE AltName: Full=Methyltransferase LAE1 {ECO:0000305};
DE EC=2.1.1.- {ECO:0000250|UniProtKB:C8VQG9};
DE AltName: Full=Velvet complex subunit LAE1 {ECO:0000305};
GN Name=LAE1 {ECO:0000303|PubMed:20572938}; ORFNames=FFUJ_00592;
OS Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae
OS and foot rot disease fungus) (Fusarium fujikuroi).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium fujikuroi species complex.
OX NCBI_TaxID=1279085;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, SUBCELLULAR LOCATION,
RP INTERACTION WITH VEL1/VEA, AND DISRUPTION PHENOTYPE.
RC STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831;
RX PubMed=20572938; DOI=10.1111/j.1365-2958.2010.07263.x;
RA Wiemann P., Brown D.W., Kleigrewe K., Bok J.W., Keller N.P., Humpf H.U.,
RA Tudzynski B.;
RT "FfVel1 and FfLae1, components of a velvet-like complex in Fusarium
RT fujikuroi, affect differentiation, secondary metabolism and virulence.";
RL Mol. Microbiol. 77:972-994(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831;
RX PubMed=23825955; DOI=10.1371/journal.ppat.1003475;
RA Wiemann P., Sieber C.M.K., von Bargen K.W., Studt L., Niehaus E.-M.,
RA Espino J.J., Huss K., Michielse C.B., Albermann S., Wagner D.,
RA Bergner S.V., Connolly L.R., Fischer A., Reuter G., Kleigrewe K., Bald T.,
RA Wingfield B.D., Ophir R., Freeman S., Hippler M., Smith K.M., Brown D.W.,
RA Proctor R.H., Muensterkoetter M., Freitag M., Humpf H.-U., Gueldener U.,
RA Tudzynski B.;
RT "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen
RT Fusarium fujikuroi reveal complex regulation of secondary metabolism and
RT novel metabolites.";
RL PLoS Pathog. 9:E1003475-E1003475(2013).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24389666; DOI=10.1007/s00253-013-5453-1;
RA Niehaus E.M., von Bargen K.W., Espino J.J., Pfannmueller A., Humpf H.U.,
RA Tudzynski B.;
RT "Characterization of the fusaric acid gene cluster in Fusarium fujikuroi.";
RL Appl. Microbiol. Biotechnol. 98:1749-1762(2014).
CC -!- FUNCTION: Methyltransferase that performs automethylation (By
CC similarity). No other methyl-accepting substrate has been identified
CC yet (By similarity). Component of the velvet transcription factor
CC complex that acts as a global regulator for secondary metabolite gene
CC expression (PubMed:20572938). Controls the expression of the
CC gibberellins gene clusters, but does not affect bikaverin production
CC (PubMed:20572938). Controls the expression of the fusaric acid gene
CC cluster (PubMed:24389666). Acts as a virulence factors during
CC infection, most likely through activation of gibberellins biosynthesis
CC (PubMed:20572938). {ECO:0000250|UniProtKB:C8VQG9,
CC ECO:0000269|PubMed:20572938, ECO:0000269|PubMed:24389666}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-methionyl-[protein] + S-adenosyl-L-methionine = S-adenosyl-
CC L-homocysteine + S-methyl-L-methionyl-[protein];
CC Xref=Rhea:RHEA:60560, Rhea:RHEA-COMP:12313, Rhea:RHEA-COMP:15592,
CC ChEBI:CHEBI:16044, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:142742; Evidence={ECO:0000250|UniProtKB:C8VQG9};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60561;
CC Evidence={ECO:0000250|UniProtKB:C8VQG9};
CC -!- SUBUNIT: Component of the heterotrimeric velvet complex composed of
CC LAE1, VEL1 and VEL2; VEL1 acting as a bridging protein between LAE1 and
CC VEL2 (By similarity). Interacts with VEL1 (PubMed:20572938).
CC {ECO:0000250|UniProtKB:C8VQG9, ECO:0000269|PubMed:20572938}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:20572938}.
CC -!- INDUCTION: Expression is regulated by the VEL1 (PubMed:20572938).
CC {ECO:0000269|PubMed:20572938}.
CC -!- DISRUPTION PHENOTYPE: Reduces gibberellins production and subsequent
CC virulence during infection (PubMed:20572938). Reduces both the fusaric
CC acid gene cluster expression and production of fusaric acid
CC (PubMed:24389666). {ECO:0000269|PubMed:20572938,
CC ECO:0000269|PubMed:24389666}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. LaeA
CC methyltransferase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CCT62818.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; FN548141; CBE54370.1; -; Genomic_DNA.
DR EMBL; HF679023; CCT62818.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; S0DQI7; -.
DR SMR; S0DQI7; -.
DR STRING; 1279085.S0DQI7; -.
DR EnsemblFungi; CCT62818; CCT62818; FFUJ_00592.
DR HOGENOM; CLU_010595_2_0_1; -.
DR Proteomes; UP000016800; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 1: Evidence at protein level;
KW Methyltransferase; Nucleus; Reference proteome; S-adenosyl-L-methionine;
KW Sporulation; Transcription; Transcription regulation; Transferase;
KW Virulence.
FT CHAIN 1..316
FT /note="Secondary metabolism regulator LAE1"
FT /id="PRO_0000435749"
SQ SEQUENCE 316 AA; 36624 MW; 4657FCFE4FFDB2DA CRC64;
MVVMPPQNSV NESEGRYLQD GFWQHGRFYG SWKPGKYLFP IDSEELNRLD IFHKVFLLAR
DNKPFLAPIR RTSPRIMDIG TGTGIWAINV AEECLSDAQI MAVDLNQIQP ALIPPGFMPK
QYDIEEPSWG PLLADCDLIH MRMLLGSIQT DLWPQVYHNA FEHLTPGIGF LEHIEVDWIP
RCDDDERPAN SAFVKWAELF LDGMDRFNRS VRVIPQEHRQ MLEATGFTDV KQEVIKAYVC
PWSADRNERE IARWFNIGLS HSLEAMSLKP LIEKLGFEAE DVRELCERAK RETCVLRYHT
YCNIHVWTAR KPGPQQ