LAEA_GIBM7
ID LAEA_GIBM7 Reviewed; 316 AA.
AC W7LAD1; W7LMB4;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 16-MAR-2016, sequence version 2.
DT 03-AUG-2022, entry version 24.
DE RecName: Full=Secondary metabolism regulator LAE1 {ECO:0000305};
DE AltName: Full=Methyltransferase LAE1 {ECO:0000305};
DE EC=2.1.1.- {ECO:0000250|UniProtKB:C8VQG9};
DE AltName: Full=Velvet complex subunit LAE1 {ECO:0000305};
GN Name=LAE1 {ECO:0000303|PubMed:22713715}; ORFNames=FVEG_00539;
OS Gibberella moniliformis (strain M3125 / FGSC 7600) (Maize ear and stalk rot
OS fungus) (Fusarium verticillioides).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium fujikuroi species complex.
OX NCBI_TaxID=334819;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M3125 / FGSC 7600;
RX PubMed=20237561; DOI=10.1038/nature08850;
RA Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT "Comparative genomics reveals mobile pathogenicity chromosomes in
RT Fusarium.";
RL Nature 464:367-373(2010).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22713715; DOI=10.1016/j.fgb.2012.06.003;
RA Butchko R.A., Brown D.W., Busman M., Tudzynski B., Wiemann P.;
RT "Lae1 regulates expression of multiple secondary metabolite gene clusters
RT in Fusarium verticillioides.";
RL Fungal Genet. Biol. 49:602-612(2012).
CC -!- FUNCTION: Methyltransferase that performs automethylation (By
CC similarity). No other methyl-accepting substrate has been identified
CC yet (By similarity). Component of the velvet transcription factor
CC complex that acts as a global regulator for secondary metabolite gene
CC expression (PubMed:22713715). Controls the expression of the bikaverin,
CC fumonisins, fusaric acid, and fusarins gene clusters; as well as two
CC clusters for which the corresponding secondary metabolite is unknown
CC (PubMed:22713715). {ECO:0000250|UniProtKB:C8VQG9,
CC ECO:0000269|PubMed:22713715}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-methionyl-[protein] + S-adenosyl-L-methionine = S-adenosyl-
CC L-homocysteine + S-methyl-L-methionyl-[protein];
CC Xref=Rhea:RHEA:60560, Rhea:RHEA-COMP:12313, Rhea:RHEA-COMP:15592,
CC ChEBI:CHEBI:16044, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:142742; Evidence={ECO:0000250|UniProtKB:C8VQG9};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60561;
CC Evidence={ECO:0000250|UniProtKB:C8VQG9};
CC -!- SUBUNIT: Component of the heterotrimeric velvet complex composed of
CC LAE1, VE1 and VELB; VE1 acting as a bridging protein between LAE1 and
CC VELB (By similarity). {ECO:0000250|UniProtKB:C8VQG9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C8VQG9}.
CC -!- DISRUPTION PHENOTYPE: Impairs production of bikaverin, fumonisins,
CC fusaric acid, and fusarins (PubMed:22713715).
CC {ECO:0000269|PubMed:22713715}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. LaeA
CC methyltransferase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EWG36563.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DS022242; EWG36563.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DS022242; EWG36564.1; -; Genomic_DNA.
DR EMBL; DS022242; EWG36565.1; -; Genomic_DNA.
DR EMBL; DS022242; EWG36566.1; -; Genomic_DNA.
DR EMBL; DS022242; EWG36567.1; -; Genomic_DNA.
DR EMBL; DS022242; EWG36568.1; -; Genomic_DNA.
DR RefSeq; XP_018742754.1; XM_018887055.1.
DR RefSeq; XP_018742755.1; XM_018887056.1.
DR RefSeq; XP_018742756.1; XM_018887057.1.
DR RefSeq; XP_018742757.1; XM_018887058.1.
DR RefSeq; XP_018742758.1; XM_018887059.1.
DR RefSeq; XP_018742759.1; XM_018887060.1.
DR AlphaFoldDB; W7LAD1; -.
DR STRING; 117187.FVEG_00539T0; -.
DR GeneID; 30058903; -.
DR KEGG; fvr:FVEG_00539; -.
DR VEuPathDB; FungiDB:FVEG_00539; -.
DR eggNOG; ENOG502QQMC; Eukaryota.
DR OrthoDB; 1047281at2759; -.
DR Proteomes; UP000009096; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 3: Inferred from homology;
KW Methyltransferase; Nucleus; Reference proteome; S-adenosyl-L-methionine;
KW Sporulation; Transcription; Transcription regulation; Transferase.
FT CHAIN 1..316
FT /note="Secondary metabolism regulator LAE1"
FT /id="PRO_0000435750"
SQ SEQUENCE 316 AA; 36707 MW; BB3373DF8E09B547 CRC64;
MVVMPPQNSV NESEGRYLQD GFWQHGRFYG SWKPGKYLFP IDSEELNRLD IFHKVFLLAR
DNKPFLAPIR RTSPRIMDIG TGTGIWAINV AEECLSDAQI MAVDLNQIQP ALIPPGFMPK
QYDIEEPSWG PLLADCDLIH MRMLLGSIQT DLWPQVYHNA FEHLAPGIGF LEHIEVDWIP
RCDDDERPAN SAFVKWAELF LDGMDRFNRR VRVTPQEHRQ MLEATGFTDI RQEVIKAYVC
PWSADRNERE IARWFNIGLS HSLEAMSLKP LIEKLGFEAE EVRELCERAK RETCVLRYHT
YCNIHVWTAR KPGPQQ
