LAEA_HYPAI
ID LAEA_HYPAI Reviewed; 349 AA.
AC G9P9X3;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 22-FEB-2012, sequence version 1.
DT 03-AUG-2022, entry version 33.
DE RecName: Full=Secondary metabolism regulator LAE1 {ECO:0000305};
DE AltName: Full=Methyltransferase LAE1 {ECO:0000305};
DE EC=2.1.1.- {ECO:0000250|UniProtKB:C8VQG9};
DE AltName: Full=Velvet complex subunit LAE1 {ECO:0000305};
GN Name=LAE1 {ECO:0000303|PubMed:23826217}; ORFNames=TRIATDRAFT_302782;
OS Hypocrea atroviridis (strain ATCC 20476 / IMI 206040) (Trichoderma
OS atroviride).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX NCBI_TaxID=452589;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 20476 / IMI 206040;
RX PubMed=21501500; DOI=10.1186/gb-2011-12-4-r40;
RA Kubicek C.P., Herrera-Estrella A., Seidl-Seiboth V., Martinez D.A.,
RA Druzhinina I.S., Thon M., Zeilinger S., Casas-Flores S., Horwitz B.A.,
RA Mukherjee P.K., Mukherjee M., Kredics L., Alcaraz L.D., Aerts A., Antal Z.,
RA Atanasova L., Cervantes-Badillo M.G., Challacombe J., Chertkov O.,
RA McCluskey K., Coulpier F., Deshpande N., von Doehren H., Ebbole D.J.,
RA Esquivel-Naranjo E.U., Fekete E., Flipphi M., Glaser F.,
RA Gomez-Rodriguez E.Y., Gruber S., Han C., Henrissat B., Hermosa R.,
RA Hernandez-Onate M., Karaffa L., Kosti I., Le Crom S., Lindquist E.,
RA Lucas S., Luebeck M., Luebeck P.S., Margeot A., Metz B., Misra M.,
RA Nevalainen H., Omann M., Packer N., Perrone G., Uresti-Rivera E.E.,
RA Salamov A., Schmoll M., Seiboth B., Shapiro H., Sukno S.,
RA Tamayo-Ramos J.A., Tisch D., Wiest A., Wilkinson H.H., Zhang M.,
RA Coutinho P.M., Kenerley C.M., Monte E., Baker S.E., Grigoriev I.V.;
RT "Comparative genome sequence analysis underscores mycoparasitism as the
RT ancestral life style of Trichoderma.";
RL Genome Biol. 12:R40.1-R40.15(2011).
RN [2]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=23826217; DOI=10.1371/journal.pone.0067144;
RA Karimi Aghcheh R., Druzhinina I.S., Kubicek C.P.;
RT "The putative protein methyltransferase LAE1 of Trichoderma atroviride is a
RT key regulator of asexual development and mycoparasitism.";
RL PLoS ONE 8:E67144-E67144(2013).
CC -!- FUNCTION: Methyltransferase that performs automethylation (By
CC similarity). No other methyl-accepting substrate has been identified
CC yet (By similarity). Component of the velvet transcription factor
CC complex that acts as a global regulator for secondary metabolite gene
CC expression (PubMed:23826217). Controls the expression of the gamma-
CC pentyl-pyrone gene clusters (PubMed:23826217). Required for the
CC expression of cellulase (PubMed:23826217). Regulates asexual
CC sporulation (conidiation) by environmental stimuli such as light and/or
CC mechanical injury (PubMed:23826217). Required for oxidative stress
CC tolerance (PubMed:23826217). Also plays a role in defense and
CC parasitism on other fungi (PubMed:23826217).
CC {ECO:0000250|UniProtKB:C8VQG9, ECO:0000269|PubMed:23826217}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-methionyl-[protein] + S-adenosyl-L-methionine = S-adenosyl-
CC L-homocysteine + S-methyl-L-methionyl-[protein];
CC Xref=Rhea:RHEA:60560, Rhea:RHEA-COMP:12313, Rhea:RHEA-COMP:15592,
CC ChEBI:CHEBI:16044, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:142742; Evidence={ECO:0000250|UniProtKB:C8VQG9};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60561;
CC Evidence={ECO:0000250|UniProtKB:C8VQG9};
CC -!- SUBUNIT: Component of the heterotrimeric velvet complex composed of
CC LAE1, VEL1 and VEL2; VEL1 acting as a bridging protein between LAE1 and
CC VEL2 (By similarity). {ECO:0000250|UniProtKB:C8VQG9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C8VQG9}.
CC -!- DISRUPTION PHENOTYPE: Leads to reduced growth rate on plates with D-
CC glucose or D-galactose, decreases the expression of mycoparasitism-
CC associated genes, and impairs conidiation (PubMed:23826217).
CC {ECO:0000269|PubMed:23826217}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. LaeA
CC methyltransferase family. {ECO:0000305}.
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DR EMBL; ABDG02000028; EHK40444.1; -; Genomic_DNA.
DR RefSeq; XP_013938600.1; XM_014083125.1.
DR AlphaFoldDB; G9P9X3; -.
DR SMR; G9P9X3; -.
DR STRING; 452589.G9P9X3; -.
DR EnsemblFungi; EHK40444; EHK40444; TRIATDRAFT_302782.
DR GeneID; 25782404; -.
DR eggNOG; ENOG502QQMC; Eukaryota.
DR HOGENOM; CLU_010595_2_0_1; -.
DR OMA; KNCDFYA; -.
DR OrthoDB; 1047281at2759; -.
DR Proteomes; UP000005426; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR SUPFAM; SSF53335; SSF53335; 1.
PE 3: Inferred from homology;
KW Methyltransferase; Nucleus; Reference proteome; S-adenosyl-L-methionine;
KW Sporulation; Transcription; Transcription regulation; Transferase;
KW Virulence.
FT CHAIN 1..349
FT /note="Secondary metabolism regulator LAE1"
FT /id="PRO_0000435755"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 349 AA; 40063 MW; 93780C930DDB1441 CRC64;
MSSRNAPSGC VAPSPATAAP PSPTNLRLTV GQSGSESANE PGGEPEERIL QDGFWEYGRF
YGNWKKGKYN FPIDKEETSR LDILHKYFIV ETEDRVTSVP LDKEGSPKIM DLGTGTGIWA
FHVVEGYIPN AQIMAVDLNQ IQPALIPRGV TTKQFDLEEP SWEPLLRDCD LIHLRLLYGS
IRDDLWADTY RKIFEHLAPG GYVEHLEIDW TPQWDGEDHP THSAIREWSQ QFHRAMHRYR
RSVKVSSEDT KRMMEAAGFT EFKETKIRCY LNPWSTDRHQ REAARWFNLA LGLGLEAMSL
MPMIDMLHMK QEDVVDLCKR VKAETCVLRY HAYFTLHTWT AKKPASPPQ
