ID   LAFA_LACJO              Reviewed;          75 AA.
AC   P24022;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2004, sequence version 2.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Bacteriocin lactacin-F subunit LafA;
DE   Flags: Precursor;
GN   Name=lafA; Synonyms=laf; OrderedLocusNames=LJ_0769;
OS   Lactobacillus johnsonii (strain CNCM I-12250 / La1 / NCC 533).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=257314;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088;
RX   PubMed=1900281; DOI=10.1128/jb.173.5.1779-1788.1991;
RA   Muriana P.M., Klaenhammer T.R.;
RT   "Cloning, phenotypic expression, and DNA sequence of the gene for lactacin
RT   F, an antimicrobial peptide produced by Lactobacillus spp.";
RL   J. Bacteriol. 173:1779-1788(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088;
RX   PubMed=8285694; DOI=10.1128/aem.59.11.3906-3915.1993;
RA   Fremaux C., Ahn C., Klaenhammer T.R.;
RT   "Molecular analysis of the lactacin F operon.";
RL   Appl. Environ. Microbiol. 59:3906-3915(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CNCM I-1225 / La1 / NCC 533;
RX   PubMed=14983040; DOI=10.1073/pnas.0307327101;
RA   Pridmore R.D., Berger B., Desiere F., Vilanova D., Barretto C.,
RA   Pittet A.-C., Zwahlen M.-C., Rouvet M., Altermann E., Barrangou R.,
RA   Mollet B., Mercenier A., Klaenhammer T., Arigoni F., Schell M.A.;
RT   "The genome sequence of the probiotic intestinal bacterium Lactobacillus
RT   johnsonii NCC 533.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:2512-2517(2004).
RN   [4]
RP   PROTEIN SEQUENCE OF 19-43.
RC   STRAIN=ATCC 11506 / JCM 1101 / NBRC 13952 / NCIMB 8795 / R-26 / VPI 11088;
RX   PubMed=1903624; DOI=10.1128/aem.57.1.114-121.1991;
RA   Muriana P.M., Klaenhammer T.R.;
RT   "Purification and partial characterization of lactacin F, a bacteriocin
RT   produced by Lactobacillus acidophilus 11088.";
RL   Appl. Environ. Microbiol. 57:114-121(1991).
CC   -!- FUNCTION: Heat stable bacteriocin active against Enterococcus faecalis
CC       and other Lactobacilli.
CC   -!- SUBUNIT: This bacteriocin depends upon the complementation of two
CC       peptides for activity: LafA and LafX. Associated with a 180 kDa
CC       bacteriocin complex.
CC   -!- SIMILARITY: Belongs to the bacteriocin class IIB family. {ECO:0000305}.
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DR   EMBL; M57961; AAA16636.1; -; Unassigned_DNA.
DR   EMBL; AE017198; AAS08588.1; -; Genomic_DNA.
DR   RefSeq; WP_011161711.1; NC_005362.1.
DR   AlphaFoldDB; P24022; -.
DR   STRING; 257314.LJ_0769; -.
DR   TCDB; 1.C.26.1.1; the lactacin x (lactacin x) family.
DR   EnsemblBacteria; AAS08588; AAS08588; LJ_0769.
DR   KEGG; ljo:LJ_0769; -.
DR   PATRIC; fig|257314.6.peg.625; -.
DR   eggNOG; ENOG50304YT; Bacteria.
DR   HOGENOM; CLU_188490_0_0_9; -.
DR   OMA; NNWAANV; -.
DR   Proteomes; UP000000581; Chromosome.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR019493; Bacteriocin_IIb_lactacin-rel.
DR   Pfam; PF10439; Bacteriocin_IIc; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Reference proteome.
FT   PROPEP          1..18
FT                   /evidence="ECO:0000269|PubMed:1903624"
FT                   /id="PRO_0000002753"
FT   CHAIN           19..75
FT                   /note="Bacteriocin lactacin-F subunit LafA"
FT                   /id="PRO_0000002754"
FT   CONFLICT        62
FT                   /note="A -> G (in Ref. 1; no nucleotide entry and 2;
FT                   AAA16636)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   75 AA;  7604 MW;  5A9234FC33434489 CRC64;
     MKQFNYLSHK DLAVVVGGRN NWQTNVGGAV GSAMIGATVG GTICGPACAV AGAHYLPILW
     TAVTAATGGF GKIRK