ID   LAFS_VIBPA              Reviewed;         242 AA.
AC   Q03474;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 126.
DE   RecName: Full=RNA polymerase sigma factor for flagellar operon;
DE   AltName: Full=Sigma-27;
DE   AltName: Full=Sigma-F factor;
GN   Name=lafS; OrderedLocusNames=VPA1555;
OS   Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=223926;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BB22;
RX   PubMed=8501040; DOI=10.1128/jb.175.11.3361-3371.1993;
RA   McCarter L.L., Wright M.E.;
RT   "Identification of genes encoding components of the swarmer cell flagellar
RT   motor and propeller and a sigma factor controlling differentiation of
RT   Vibrio parahaemolyticus.";
RL   J. Bacteriol. 175:3361-3371(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RIMD 2210633;
RX   PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA   Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA   Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA   Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT   "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT   distinct from that of V. cholerae.";
RL   Lancet 361:743-749(2003).
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. This alternative sigma factor is specific for the flagellin
CC       gene (fliC) expression.
CC   -!- INDUCTION: Under conditions in which the polar flagellum is not
CC       functional.
CC   -!- SIMILARITY: Belongs to the sigma-70 factor family. {ECO:0000305}.
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DR   EMBL; U20541; AAA62353.1; -; Genomic_DNA.
DR   EMBL; U52957; AAB07356.1; -; Genomic_DNA.
DR   EMBL; BA000032; BAC62898.1; -; Genomic_DNA.
DR   PIR; D40590; D40590.
DR   RefSeq; NP_801065.1; NC_004605.1.
DR   RefSeq; WP_005462772.1; NC_004605.1.
DR   AlphaFoldDB; Q03474; -.
DR   SMR; Q03474; -.
DR   STRING; 223926.28809957; -.
DR   EnsemblBacteria; BAC62898; BAC62898; BAC62898.
DR   GeneID; 1192251; -.
DR   KEGG; vpa:VPA1555; -.
DR   PATRIC; fig|223926.6.peg.4477; -.
DR   eggNOG; COG1191; Bacteria.
DR   HOGENOM; CLU_014793_8_1_6; -.
DR   OMA; FIRDKTW; -.
DR   Proteomes; UP000002493; Chromosome 2.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:InterPro.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription, initiation; IEA:InterPro.
DR   InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR   InterPro; IPR000943; RNA_pol_sigma70.
DR   InterPro; IPR007627; RNA_pol_sigma70_r2.
DR   InterPro; IPR007630; RNA_pol_sigma70_r4.
DR   InterPro; IPR012845; RNA_pol_sigma_FliA_WhiG.
DR   InterPro; IPR013325; RNA_pol_sigma_r2.
DR   InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR   Pfam; PF04542; Sigma70_r2; 1.
DR   Pfam; PF04545; Sigma70_r4; 1.
DR   SUPFAM; SSF88659; SSF88659; 2.
DR   SUPFAM; SSF88946; SSF88946; 1.
DR   TIGRFAMs; TIGR02479; FliA_WhiG; 1.
DR   TIGRFAMs; TIGR02937; sigma70-ECF; 1.
DR   PROSITE; PS00716; SIGMA70_2; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Reference proteome; Sigma factor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..242
FT                   /note="RNA polymerase sigma factor for flagellar operon"
FT                   /id="PRO_0000093988"
FT   DNA_BIND        211..230
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MOTIF           55..68
FT                   /note="Polymerase core binding"
SQ   SEQUENCE   242 AA;  27835 MW;  39A3C7152DE2FC95 CRC64;
     MLDMNPQETY TAPEEVNTPS RPIDENALLQ RHQVMVKRVV NQLRVHATSH CSIEDMQQIG
     LIALVEAGRR YGDIDDTHFP AFAVCRVRGA ILDELRRLDW RSRKTRQQAH ELNDVTRDLT
     RSLGRMPTDS EIIKALGTDE QDYYNRQNAA LAGEMQSLDQ LMENSTDSHF GGQYDGMEHE
     HIRRSLDSAL GRLSKRDQLL LTLFYQHELN LHEIALVLDL TPPRICQLHK QALKQLNQLM
     SS